Murine EPS Drug Doses
IP doses are calibrated to give 0.2 ml for a 30 g mouse
Atropine:
0.5 mg/g = 15 mg IP (Mix 0.2 ml of stock solution in a total of 1 ml)
Atropine stock in vial = 0.4 mg/ml
Carbamyl choline:
500 ng/g = 15 mg IP (Mix 7.5 mg into 10 ml for a x10 stock solution)
Cefazolin:
200 mg/g/day = 6 mg/day in two divided doses = 3 mg IP bid (Mix 24 mg into 1.6 ml for 8 individual doses)
Digoxin:
20 ng/g bid = 0.6 mg IP bid on Day 1 (Mix 0.2 ml of stock in a total of 10 ml). On Day 2 give 10 ng/g bid = 0.3 mg IP bid (0.1 ml IP)
Digoxin stock in vial = 0.1 mg/ml
Isoproterenol:
3 ng/g = 90 ng IP (Mix 0.25 ml of x100 stock in a total of 1 ml)
Isoproterenol x100 stock = 2 mg/ml
Propranolol:
1 mg/g = 30 mg IP (Mix 0.15 ml of stock in a total of 1 ml)
Propranolol stock in vial = 1 mg/ml
IV doses are calibrated to give 0.05 ml for a 30 g mouse
Isoproterenol:
1 ng/g = 30 ng IV (Mix 0.3 ml of x100 stock in a total of 1 ml)
Isoproterenol x100 stock = 2 mg/ml
Procainamide:
30 mg/g = 900 mg IV (Add 2.2 ml of procainamide stock to 10 ml for a total of 12.2 ml which gives a concentration of 18 mg/ml)
Procainamide stock in vial = 100 mg/ml
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