Cell Culture Core
Erle Robertson, PhD
Professor of Microbiology
Director, Tumor Virology Program
Abramson Cancer Center
Hiroshi Nakagawa, MD, PhD
Research Associate Professor of Medicine
Hem Chandra Jha, PhD
Johnson Pavilion 202A
(215) 746-0116 phone
(215) 898-9557 fax
Meenhard Herlyn, DVM, D.Sc.
The Cell Culture Core maintains a centralized repository of cells and reagents pertinent to digestive, liver and pancreatic disease research. It also provides training (especially for students and postdoc fellows) for labs in new cell culture (2D and 3D) techniques.
Cells lines are established from freshly obtained surgical specimens, all aspects of which are approved by the University of Pennsylvania Institutional Review Board. Adenoviral, retroviral and lentiviral constructs are created, propagated and maintained under institutional guidelines for biohazardous materials. Provided is a listing of items currently carried by the Cell Culture Core:
- Lists of available GI cancers and engineered human and mouse esophageal epithelial cells.
- Protocol for human esophageal epithelial cell culture.
- Protocol for mouse esophageal epithelial cell culture.
- Protocol for soybean trypsin inhibitor preparation used for mouse and human esophageal cell culture.
- Protocol for organotypic 3D culture.
- Basic Sterile Techniques.
Normal human cell lines
- colonic enterocytes
- esophageal keratinocytes
- fibrobasts and smooth muscle cells
- endothelial cells
- Organotypic (three dimensional) culture systems: colonocytes or esophageal keratinocytes with a substrate of fibroblasts, smooth muscle cells and endothelial cells.
Malignant Cell Lines
Well-characterized human cell lines originating from carcinomas of the following:
- colon and rectum
Also, techniques are available to isolate fibroblasts.
Techniques / Education
Training available in the establishment and maintenance of:
- three-dimensional organotypic cultures of normal colon, esophagus, colon, and blood vessels
- permanent cell lines from patients' lesions and from tumors grown in immunodeficient mice
- design and use of adenoviral, retroviral and lentiviral vectors