Molecular Biology Core
Director
Gary Wu, M.D.
Technical Director
Sue Keilbaugh, Ph.D.
Lab: 630 CRB
Phone: 215-573-1879
Fax: 215-573-2024
- Center Member Application (Word doc)
The Molecular Biology Core maintains a library of digestive and liver reagents, provides advice and assistance in molecular techniques, and subsidizes the cost of specific Penn-based technical services to assist Center members in their research projects.
Acrobat Reader needed to view or print reagent summary and detail, and order forms.
Services
Digestive Disease Reagent Bank
CDNAs, expression vectors, adenoviral vectors relevant to digestive and liver research provided free of charge to members of the Center for Molecular Studies in Digestive and Liver Disease.
Reagents Available:
Reagent:
- Order Form (pdf)
- Donations to core for shared use (pdf)
Mouse Database
The Center for Molecular Studies in Digestive and Liver Diseases is compiling a database of "mouse reagents". The purpose of this database is to facilitate collaboration in mouse research between center investigators. The database is an informational resource only. It is located on the Penn intranet; therefore, this information is available only to members of the Penn community. The Center will not serve as a repository of the mouse lines, and collaboration must be arranged directly between individual investigators.
- Transgenic List
- Knockout List
- Submission Form
RNA Source Bank
RNA samples from various digestive organs provided to Center members. Each RNA sample has undergone initial characterization by northern blot analysis. Contact Sue Keilbaugh for more information.
Equipment available in room 630 CRB for use by all Center Members.
Image and Sequence Analysis
- Molecular Dynamics 840 Phosphorimager
- MacVector 7.0 Software
- Lasergene (DNAStar) software
- Lasergene S.O.
- Prism (Graphpad)
- Microarray Suite
- BioRad 1000 Gel Dock
- Canon CanoScan LIDE90
- Lasergraphics Slidemaker
- Dynex Luminometer
- Orion Luminometer
- Agilent Bioanalyzer (in Penn's Microarray Facility)
- BioRad ChemiDoc/GelDock
- Becton Dickinson FACSCalibur Flowcytometer
- Applied Biosystems 2720 Thermal Cycler
Bioinformatics Support
University Bioinformatics Core
http://www.bioinformatics.upenn.edu
John Tobias, PhD Tel: (215) 573-4791
email: jtobias@pcbi.upenn.edu
Penn Bioinformatics Core personnel provide consulting to enable investigators to frame research questions in genomics terms and identify bioinformatics solutions to those questions. Core personnel are then available to facilitate these solutions which may involve developing databases and software tools as well as provide training and support in the use of these tools.
Biostatistics Support
Center for Clinical Epidemiology & Biostatistics
http://www.cceb.upenn.edu
Colleen Brensinger, MS Tel: (215) 573-4193
email: cbrensin@cceb.upenn.edu
Center members will now be able to obtain Biostatistical support for the development of study design and data analysis through the CCEB by appointment with Colleen Brensinger, a Biostatistician in the Penn Center for Clinical Epidemiology and Biostatistics
Affimetrix microarrays: credit for hybridization and scanning of up to five Affimetrix microarray chips, and 10% discount on all other services at the Penn Microarray Core. Contact Don Baldwin, PhD regarding usage, and the GI Center Administrator to obain credit or discount.
Quantitative Real-Time PCR: for verifying and quantifying expression patterns of specific genes identified by mircoarray gene profiling. An ABI 7000 Quantitative PCR Machine is available in 630 CRB for use by Center Investigators at a cost of $25.00 per run. Please contact the Sue Keilbaugh for more information on the use of this instrument.