Molecular Pathology & Imaging Core (MPIC)
Director
Jonathan P. Katz, MD
Associate Director
Antonia Sepulveda, MD, PhD
Research Specialist
Daniela Budo
Research Lab Technician
Krysta Kelly
Core: 602 CRB and 675 CRB
Phone: 215-898-8060
Fax: 215-573-2024
The Molecular Pathology & Imaging Core provides a service component and technical expertise to digestive and liver research projects. It is funded by the Center for Molecular Studies in Digestive and Liver Diseases, the Metabolic Development Program Project, and the Mechanisms of Esophageal Carcinogenesis Program Project. Please send us questions or suggestions for new services.
Note: Molecular Pathology & Imaging Core Services are available only to Members of the Center.
Equipment sign-out
Click here to reserve Microscopes and other Loan-out items
On-line tissue order form
Click here to order Tissue Specimens and Services.
Protocols
Tissue Processing: Background Tips and Tricks (word doc)
- Pepsin-digest Immunohistochemistry
- Protease-digest Immunohistochemistry
- Trypsin-digest Immunohistochemistry
- Standard (pH3) Immunohistochemistry
- Standard (pH6) Immunohistochemistry
- Standard (pH8) Immunohistochemistry
- Sudan Black method IF on paraffin sections
Center Member Application (Word doc)
Charges
| Services | Charges |
| Tissue embedding | $4.00 / block |
| Sectioning up to 7 slides with 1 or 2 H&E | $14.00 / block |
| Additional sections | $2.00 / slide |
| Special Stains: PAS, Trichrome, Alcian Blue, Best's Carmine, Oil Red O |
$4.00 / slide |
| Animal dissection | $25.00/ dissection |
| Spinning disk confocal microscopy | $20.00/hour ($100.00 maximum) |
| Laser capture microscopy | $20.00 /hour |
| Tissue Microarray construction | $5.00/ tissue core |
MPIC Workstations
- Two Nikon E600 microscopes with Brightfield, Nomarski and Fluorescent optics. One of these microscopes is also equipped with a Z-axis drive for three-dimensional imaging and subsequent deconvolution. The microscopes are both equipped with CCD cameras and IPLab image analysis software.
- A third Nikon E600 equipped with Brightfield and Nomarski optics. This microscope is equipped with a dual viewing head, a CCD camera and IPLab image analysis software.
- A Nikon Eclipse Ti -U microscope attached to an upgraded Yokagawa CSU-10 spinning-disk confocal head with a custom-designed environmental chamber for live-cell imaging. This microscope is equipped with a CCD camera and MetaMorph Image Analysis Software. Please note that Tuesdays through Thursdays are reserved for routine imaging and image capture; Fridays through Mondays are reserved for live-cell imaging experiments, which must be discussed in advance with the Technical Director.
- Inverted Leica DM IRB microscope with Brightfield, Phase Contrast and Fluorescent optics. Equipped with CCD camera and IPLab image analysis software. This microscope is equipped with CCD camera and IPLab image analysis software.
- Nikon Eclipse TE2000-S inverted Laser Capture Microscope with Brightfield, Phase Contrast, Fluorescent optics and imaging.
- Nikon SMZ-U dissecting microscope with imaging.
- Five Macintosh computers with software for Image Analysis, 3-D Image Deconvolution and PhotoShop.
- Nikon E400 microscope that can be signed out of the Core for use in client labs.
- Nikon SMZ645 dissecting stereo microscope and fiber optic illuminator that can be signed out of the Core for use in client labs.
Multi-User Reagents
No charge to members for reagents listed. Please discuss additional reagents that would be of general use to members with Gary Swain for potential addition to the list.
Reagent List (Word doc)
Other free services
- Trouble-shooting for immunohistochemistry
- Training of lab personnel in immunohistochemistry, microscopy, imaging, dissections, and all basic histochemical methods
- Use of core lab space and equipment listed below
- Incubator/oven (35-75 degree range)
- Mouse developmental tissue bank (users are charged only for the cost of the slides)
- Balance, pH meter, stir plate, vortex, water baths, etc
- Use of reference library