Morphology Core
Director
Jonathan P. Katz, MD
Associate Director
TBD
Technical Director
Gary P. Swain, PhD
Research Specialists
Shukriyyah Mitchell
Daniela Budo
Technicians
Jaclyn Twaddle
Core: 602 CRB
Lab: 672 CRB
Phone: 215-898-8060
Fax: 215-573-2024
- Center Member Application (Word doc)
The Morphology Core provides a service component and technical expertise to digestive and liver research projects. It is funded by the Center for Molecular Studies in Digestive and Liver Diseases, the Metabolic Development Program Project, and the Mechanisms of Esophageal Carcinogenesis Program Project.. Stock lists and order forms are linked below. Please send us questions or suggestions for new services.
Order Forms Acrobat Reader needed to view or print.
- Cryo or paraffin tissue (pdf)
- Mouse Tissue (pdf)
Reference Library
| Services | Charges |
| Animal dissection and tissue embedding | $25.00/ dissection; animal procurement addtl. |
| Tissue embedding without dissection | 3.00 / block |
| Sectioning up to 7 slides with 1 or 2 H&E | 10.00 / block |
| Additional sections | 1.50 / slide |
Special Stains
| PAS, Trichrome, Alcian Blue, Best's Carmine, Oil Red O |
4.00 / slide |
Mouse Developmental Tissue Bank Embryos
| (E8.5 - E18.5) cryo and paraffin embeded, paraformaldehyde fixed; up to 7 slides with 1 or 2 H&E | 10.00 / embryo |
| Special breeding/dissection of embryos | 20.00 / dissection; animal procurement addtl. |
| Postnatal tissue (D0 - D25) paraffin embedded, formalin fixed; up to 7 slides with 1 or 2 H&E | 10.00 / block |
| Additional sections | 1.50 / slide |
New Services
| Laser capture microscopy | 25.00 /hour |
| Tissue Microarray construction | 5.00/ tissue core |
Morphology Core Workstations
- Two Nikon E600 microscopes with Brightfield, Nomarski and Fluorescent optics. One of these microscopes is also equipped with a Z-axis drive for three-dimensional imaging and subsequent deconvolution. Both equipped with CCD camera and IPLab image analysis software.
- A third Nikon E600 equipped with Brightfield and Nomarski optics. This microscope is equipped with a dual viewing head. Equipped with CCD camera and IPLab image analysis software.
- Inverted Leica DM IRB microscope with Brightfield, Phase Contrast and Fluorescent optics. Equipped with CCD camera and IPLab image analysis software. Equipped with CCD camera and IPLab image analysis software.
- Nikon Eclipse TE2000-S inverted Laser Capture Microscope with Brightfield, Phase Contrast, Fluorescent optics and imaging.
- Nikon SMZ-U dissecting microscope with imaging.
- Five Macintosh computers with software for Image Analysis, 3-D Image Deconvolution and PhotoShop.
- Nikon E400 microscope that can be signed out of the Core for use in client labs.
- Nikon SMZ645 dissecting stereo microscope and fiber optic illuminator that can be signed out of the Core for use in client labs.
Multi-User Reagents
No charge to members for reagents listed. Please discuss additional reagents that would be of general use to members with Gary Swain for potential addition to the list.
Reagent List (Word doc)
Other services, still free of charge
- Trouble-shooting for immunohistochemistry
- Training of lab personnel in immunohistochemistry, microscopy, imaging, dissections, and all basic histochemical methods
- Use of core lab space and equipment listed below
- Incubator/oven (35-75 degree range) Dissecting microscope
- Balance, pH meter, stir plate, vortex, water baths, etc