Nucleic Acid Facility

All the primers listed below are HPLC purified and checked by CE to insure the high quality. Primers are packaged in vials of 1 OD unit each. The cost is $10/ primer. If a common primer you require is not listed,  it will be made available to you free of charge.

Standard
Primers

Sequence

Length

Tm
( C)

MW

e
*
(OD/mmol)

T7 Promoter 5'-TAA TAC GAC TCA CTA TAG GG-3' 20-mer

51

6125

205

T3 Promoter 5'-CAA TTA ACC CTC ACT AAA GG-3' 20-mer

51

6054

203

M13 Forward (-20) 5'-GTA AAA CGA CGG CCA GTG-3' 18-mer

54

5558

188

M13 Forward (-41) 5'-GGT TTT CCC AGT CAC GAC-3' 18-mer

54

5451

163

M13 Reverse (-27) 5'-GGA AAC AGC TAT GAC CAT G-3' 19-mer

53

5846

200

M13 Reverse (-48) 5'-AGC GGA TAA CAA TTT CAC AC-3' 20-mer

51

6094

207

SP6 Promoter 5'-TAC GAT TTA GGT GAC ACT ATA G-3' 22-mer

53

6773

225

pBluescript SK 5'-CGC TCT AGA ACT AGT GGA TC-3' 20-mer

55

6117

194

pBluescript KS 5'-CTC GAG GTC GAC GGT ATC G-3' 19-mer

59

5854

180

Lambda gt11 (forward) 5'-GGT GGC GAC GAC TCC TGG AGC CCG-3' 24-mer

71

7396

223

Lambda gt11 (reverse) 5'-TTG ACA CCA GAC CAA CTG GTA ATG-3' 24-mer

59

7346

243

Lambda gt10 (forward) 5'-AGC AAG TTC AGC CTG GTT AAG-3' 21-mer

56

6470

212

Lambda gt10 (reverse) 5'-CTT ATG AGT ATT TCT TCC AGG GTA-3' 24-mer

55

7349

227

pBR322 Bam HI, CW 5'-CAC TAT CGA CTA CGC GAT CA-3' 20-mer

55

6046

192

pBR322 Bam HI, CCW 5'-ATG CGT CCG GCG TAG A-3' 16-mer

52

4922

155

pBR322 Eco RI, CW 5'-GTA TCA CGA GGC CCT T-3' 16-mer

50

4857

148

pBR322 Eco RI, CCW 5'-GAT AAG CTG TCA AAC-3' 15-mer

42

4584

158

pBR322 HIND III, CW 5'-GAC AGC TTA TCA TCG-3' 15-mer

44

4552

145

pBR322 HIND III, CCW 5'-GCA ATT TAA CTG TGA T-3' 16-mer

42

4895

162

pBR322 Pst I, CW 5'-GCT AGA GTA AGT AGT T-3' 16-mer

44

4960

168

pBR322 Pst I, CCW 5'-AAC GAC GAG CGT GAC-3' 15-mer

48

4611

156

pBR322 Sal I, CW 5'-ATG CAG GAG TCG CAT-3' 15-mer

46

4617

152

pBR322 Sal I, CCW 5'-AGT CAT GCC CCG CGC-3' 15-mer

52

4514

132

Random Hexamer 5'-NNNNNN-3' 6-mer

58

1792

12

PolydT20 5'-TTT TTT TTT TTT TTT TTT TT-3' 20-mer

35

6022

158

* Extinction Coefficient