Anti-NOGO Promotes Regeneration and/or
Sprouting in CNS
Ipsilateral
Corticospinal Tract
•Transplant IN-1-secreting hybridoma subventricularly.
•Perform dorsal over-hemisection to cut the corticospinal tract.
• After 30-60 days, inject anterograde label in motor cortex.
•Detect axon terminals up to 11 mm caudal to the hemisection in IN-1 treated rats, but only 1 mm in control hybridoma recipients.
•Subsequent studies suggested that many of the nerve terminals had not regenerated but had sprouted across the midline from unlesioned ipsilateral corticospinal pathways.
Schnell L, Schwab ME. Axonal regeneration in the rat spinal cord produced by an antibody against myelin-associated neurite growth inhibitors. Nature 343:269-72, 1990.
Inject
BDA
