Perelman School of Medicine at the University of Pennsylvania

McKay Orthopaedic Research Laboratory

Shore Laboratory

Vitali Y. Lounev Ph.D.

Vitali Y. Lounev Ph.D.

Senior Research Investigator
University of Pennsylvania

Hometown: Minsk, Belarus

Education:MAPC (Multipotent Adult Progenitor Cells) Training Program, 2004, Stem Cell Institute, University of Minnesota, Minneapolis, MN
M.S., Belarusian State University, Minsk, Belarus, 1981
Ph.D., Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia, 1989

Areas of Special Interest: Origin of cells that participate in heterotopic ossification

Peer-Reviewed Publications:
Kaplan FS, Lounev VY, Wang H, Pignolo RJ, Shore EM. Fibrodysplasia ossificans progressiva: a blueprint for metamorphosis. Ann N Y Acad Sci. 2011 Nov;1237:5-10.

Kan L, Lounev VY, Pignolo RJ, Duan L, Liu Y, Stock SR, McGuire TL, Lu B, Gerard NP, Shore EM, Kaplan FS, Kessler JA. Substance P signaling mediates BMP-dependent heterotopic ossification. J Cell Biochem. 2011 Oct;112(10):2759-72.

Medici D, Shore EM, Lounev VY, Kaplan FS, Kalluri R, Olsen BR. Conversion of vascular endothelial cells into multipotent stem-like cells. Nat Med. 2010 Dec;16(12):1400-6. Erratum in: Nat Med. 2011 Apr;17(4):514.

Kaplan FS, Seemann P, Haupt J, Xu M, Lounev VY, Mullins M, Shore EM. Investigations of activated ACVR1/ALK2, a bone morphogenetic protein type I receptor, that causes fibrodysplasia ossificans progressiva. Methods Enzymol. 2010;484:357-73.

Lounev VY, Ramachandran R, Wosczyna MN, Yamamoto M, Maidment AD, Shore EM, Glaser DL, Goldhamer DJ, Kaplan FS. Identification of progenitor cells that contribute to heterotopic skeletogenesis. J Bone Joint Surg Am 2009 Mar 1;91(3):652-63.

Kaplan FS, Shen Q, Lounev V, Seemann P, Groppe J, Katagiri T, Pignolo RJ, Shore EM. Skeletal metamorphosis in fibrodysplasia ossificans progressiva (FOP). J Bone Miner Metab 2008;26(6):521-30.

Markov V., Kusumi K., Tadesse M.G., William D.A., Hall D.M., Lounev V., Carlton A., Leonard J., Cohen R.I., Rappaport E.F., Saitta B. Identification of cord blood-derived mesenchymal stem/stromal cell populations with distinct growth kinetics, differentiation potentials, and gene expression profiles. Stem Cells Dev 2007 Feb;16(1):53-73.

Fidel P.L.Jr., Barousse M., Lounev V., Espinosa T., Chesson R.R., Dunlap K. Local immune responsiveness following intravaginal challenge with Candida antigen in adult women at different stages of the menstrual cycle. Med Mycol 2003 Apr;41(2):97-109.

Fidel PL Jr, Wormley FL Jr, Chaiban J, Chesson RR, Lounev V. Analysis of the CD4 protein on human vaginal T lymphocytes. Am J Reprod Immunol 2001 Apr;45(4):200-4.

Martsev SP, Preygerzon VA, Mel'nikova YI, Kravchuk ZI, Ponomarev GV, Lunev VE, Savitsky AP. Modification of monoclonal and polyclonal IgG with palladium (II) coproporphyrin I: stimulatory and inhibitory functional effects induced by two different methods. J Immunol Methods 1995 Oct 26;186(2):293-304.

Mel'nikova IaI, Lunev VE, Preigerzon VA, Luneva NM, Koshkin SA, Rodionov MA, Martsev SP. Monoclonal antibodies to human spleen ferritin. II. Localization of epitopes and quantitative parameters of antigen binding. Biokhimiia 1993 May;58(5):759-71. Russian.

Lunev VE, Mel'nikova IaI, Koshkin SA, Luneva NM, Cherkesova TS, Vasilevskaia IA, Pre?gerzon VA, Martsev SP. Monoclonal antibodies to human spleen ferritin. I. Preparation and study of the interaction with isoferritin and apoferritin. Biokhimiia 1993 May;58(5):745-58. Russian.

Bespalov IA, Shiianov PA, Lukashevich LV, Lunev VE, Tribush SS, Gaponova GI, Deev SM. Preparation of single-chained antibodies to human ferritin in Escherichia coli. Mol Biol (Mosk) 1993 Mar-Apr;27(2):451-60. Russian.

Skrivelis VA, Bundulis IuP, Paniutich AV, Voitenok NN, Lunev VE, Nesmeianov VA, Tsimanis AIu, Gren EIa. Localization of an antigenic determinant of recombinant interleukin-2, recognized by monoclonal antibody 13B1. Bioorg Khim 1991 Jun;17(6):725-31. Russian.

Lunev VE, Lukin YuV, Kazennykh NV, Belyaev SV, Zubov VP, Nesmeyanov VA. Latex-agglutination analysis of human recombinant interleukin-2 with monoclonal antibodies. Biomed Sci 1990 Jan;1(1):68-72.

Onoprienko LV, Mikhaleva II, Lunev VE, Nesmeianov VA, Ivanov VT. Synthesis and immunochemical properties of peptides corresponding to sequences 59-72 and 25-36 of human interleukin-2. Bioorg Khim 1989 Jul;15(7):908-21. Russian.

Conference Papers and Abstracts:
Lounev V.Y., Shore E.M., Glaser D.L., Kaplan F.S. Connective tissue progenitor cell contribution to ectopic skeletogenesis. 30th ASBMR Annual Meeting, Montreal, Canada, September 12-16, 2008, Sa044.

Lounev V.Y., Shore E.M., Kan L., Goldhamer D.J., Kaplan F.S., Glaser D.L. Endothelial/hematopoietic (Tek+) cell lineages participates in heterotopic ossification. 6th ISSCR Annual Meeting, Philadelphia, PA, June 11-14, 2008.

Tai CY, Strande LF, Lounev V Hadeed JG, Eydelman R, Matthews MS, Marra SW, Hewitt CW. Vascularized bone marrow transplantation: A new surgical approach to bone marrow transplantation induces immunological tolerance. American Transplant Congress 2005, Seattle, WA, May 21-25, 2005.

Tai C.Y., Strande L.F., Hadeed J.G., Schettino M.C., Lounev V., Matthews M.S., Hewitt C.W. Peripheral CD4+CD8+ Double-positive Lymphocyte and Tolerance Induction in a Vascularized Bone Marrow Transplant Model. The Annual Meeting of New Jersey Society of Plastic Surgeons, Jamesburg, NJ, April 16, 2005.

Lounev V.Y., Markov V., Saitta B., Cohen R.I. Neural potential of human adult stem cells derived from bone marrow and umbilical cord blood. Stem Cell Research in New Jersey. An Inaugural Symposium. November 11, 2004. New Brunswick, New Jersey. A2.

Lounev V.Y., Markov V., Saitta B., Moscatello D.K., Cohen R.I. Comparative neural potential of human adult stem cells derived from bone marrow, lipoaspirate, and umbilical cord blood. 2nd Annual meeting of ISSCR, Boston, MA, June 10-13, 2004, 124.

Markov V., Filipovic D., Lounev V., Cohen R., Saitta B. Identification of two distinct mesenchymal stem cell populations in human cord blood. Keystone Symposia. Stem Cells. Keystone, CO. January 23-29, 2004. 402.

Moscatello D.K., Lounev V.Y., Cohen R.I., Lawrence N. Novel methods for the isolation and culture of adipose stromal cells from tumescent liposuction procedures. 43rd Annual Meeting of American Society for Cell Biology. San Francisco, CA, December 13-17, 2003. 520a.

Lounev V., Sykulev Y. Factors that influence activation, survival, and death of human CD8+ T lymphocytes in vitro. 7th National Symposium: Basic Aspects of Vaccines. Bethesda, MD, May 2-4, 2001, B1.

Wormley F.L.Jr., Lounev V.Y., Fidel P.L.Jr. Characterization of phenotypic distinct murine and human vaginal T cells. 2000 Keystone Symposia, Innate and acquired immunity at mucosal surfaces, Taos, New Mexico. Jan18-23, 2000, 77.

Lounev V.Y., Fidel P.L. Characterization of human vaginal T cells. 99th General Meeting of the American Society for Microbiology. Chicago, IL, May 30-June 3, 1999, E-54.

Book Chapters:
Andria L. Culbert, Salin A. Chakkalakal, Michael R. Convente, Vitali Y. Lounev, Frederick S. Kaplan and Eileen M. Shore. "Fibrodysplasia (Myositis) Ossificans Progressiva" In R.V. Thakker, M.P. Whyte, J. Eisman, T. Igarashi, eds. Genetics of Bone Biology and Skeletal Disease. Elsevier Science, 2012, pp 375-394.

Patents:
Avot A.Ya., Apsalon U.R., Bundulis Yu.P., Varivotskaya N.V., Voitenok N.N., Gren E.Ya., Lunev V.Y., Martinovich A.E., Onoprienko L.V., Romanchikova N.V., Scrivelis V.A., Tsimanis A.Yu. (1987) Recombinant plasmid DNA pAC 263-33 for human interleukin 2. Method of construction and strain E.coli - the producent of interleukin 2. USSR Patent No. 1440033.

Ovchinnikov Yu.A., Lunev V.Y., Nesmeyanov V.A., Kazennych N.V., Belyaev S.V. (1988) Hybridoma used for preparation of monoclonal antibodies to human interleukin 2. USSR Patent No. 1472498.

Ovchinnikov Yu.A., Lunev V.Y., Nesmeyanov V.A., Kazennych N.V., Belyaev S.V., Tsimanis A.Yu., Onoprienko L.V., Michalyova I.I. (1988) Hybridoma used for preparation of monoclonal antibodies to human interleukin 2. USSR Patent No. 1472499.