Protocols, Movies, and Other Files

Lab Meeting Calendar: Here

Protocols

• Actin Preparation from Acetone Powder
• Calmodulin Preparation
• Biotinylated Myo1 Motility Assay
• Cell Fixation and Immunostaining (CSB Method)
• Cell Fixation and Immunostaining (PBS Method)
• Monoclonal Antibody Purification
• SDS PAGE Buffers
• Sucrose Loaded Vesicle Preparation

Biochemical Kinetics Protocols

• Transient Kinetic Techniques (De La Cruz & Ostap)
• Simple Ligand-Binding Equations (How do I calculate the Kd?)
• Steady-State ATPase Rate Measurement (NADH Linked Assay)

Movies

• Selected Movies from Hokanson & Ostap (Mol. Biol. Cell. 17:4856-4865)
  • TIRF/FRAP Movie#1 of GFP-myo1c-tail
  • TIRF/FRAP Movie#2 of GFP-myo1c-tail
  • Time Lapse of NRK Cell expressing full length GFP-myo1c
• Animation relating myosin kinetics to duty ratio (Curr. Opin. Cell Biol. 16:61-67)
  • Duty Ratio Animation