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Leica DMIRBE (inverted) FRET/fluorescence/DIC microscope (Freedman)

Leica DMIRBE (inverted) FRET/fluorescence/DIC microscope (Freedman) - allows single cell ratiometric measurements of cytoplasmic (Fura-2) and ER calcium (FRET). Can analyze protein-protein interactions by FRET.  Attached UV flash lamp allows photo-activation of IP3 to study signal transduction mechanisms in single cells.

Scope location: 389 Old Vet Bldg., 3800 Spruce St.
Contact: Bruce Freedman, 215-573-8218, bruce@vet.upenn.edu           

  • Leica inverted DMIRBE stand
  • Fluorescence and DIC capability
  • Objectives:
    • Plan APO, 100X 1.4-0.7 NA, oil
    • Plan APO, 63X 1.2 NA, water
    • N Plan, 10X, 0.25 NA, dry
    • HCX Plan Fluotar, 40X, 0.75 NA, dry
    • PLAN Fluotar, 40X, 1.0-0.5 NA, oil
    • Olympus UApo/340, 40X, 1.35 NA, oil (for photo-uncaging).
  • Excitation and Emission computer controlled filter wheels allow emission and excitation ratiometric measurements of cytoplasmic and ER calcium.
  • Widefield FRET (CFP/YFP) capability
  • Fluorescence light source: Mercury Arc lamp
  • Picospritzer/microinjections system for microapplication of reagents onto or into single cells
  • RAPP UV flash lamp – can photo-activate caged compounds in single cells
  • Hamamatsu Orca ER camera
  • Acquisition Software: Metamorph/Metafluor
  • Motorized z-focus
  • Equipped with patch clamp to record ion channel activity
  • Open chamber heated stage