Home » Nikon TE200 Inverted Widefield Fluorescence Microscope
Nikon TE200 Inverted Widefield Fluorescence Microscope
Nikon TE200 Inverted Widefield Fluorescence Microscope (Freedman) – used primarily to obtain ratiometric (Fura-2) measurements of intracellular calcium in single cells.
Scope location: Suite 389E Old Vet Bldg, 3800 Spruce St.
Contact: Bruce Freedman, 215-573-8218, bruce@vet.upenn.edu
- Objectives:
- Plan Fluor, 40X, 1.3 NA, oil
- Plan Fluor, 100X, 0.5-1.3 NA, oil
- Plan Fluor, 40X, 0.65 NA, dry
- TILL computer controlled high speed switching monochromator (260nm-700nm, freq up to 300x/sec)
- Xenon lamp
- Open chamber heated stage
- Patch clamp recording system
- Picospritzer for application of reagents onto single cells
- Hamamatsu Intensified CCD (video) camera
- Software: Metamorph/Metafluor