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Nikon TE200 Inverted Widefield Fluorescence Microscope

Nikon TE200 Inverted Widefield Fluorescence Microscope (Freedman) – used primarily to obtain ratiometric (Fura-2) measurements of intracellular calcium in single cells.

Scope location: Suite 389E Old Vet Bldg, 3800 Spruce St.
Contact: Bruce Freedman, 215-573-8218, bruce@vet.upenn.edu           

  • Objectives:
    • Plan Fluor, 40X, 1.3 NA, oil
    • Plan Fluor, 100X, 0.5-1.3 NA, oil
    • Plan Fluor, 40X, 0.65 NA, dry
  • TILL computer controlled high speed switching monochromator (260nm-700nm, freq up to 300x/sec)
  • Xenon lamp
  • Open chamber heated stage
  • Patch clamp recording system
  • Picospritzer for application of reagents onto single cells
  • Hamamatsu Intensified CCD (video) camera
  • Software: Metamorph/Metafluor