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Olympus with TIRF and Optical Trapping Capabilities
Olympus with TIRF and Optical Trapping Capabilities (Baumgart) – using a Bioptechs temperature control system, this scope allows live-cell imaging in TIRF mode. Additionally equipped with an optical trapping system to measure the mechanical properties of membrane vesicles.
Scope location: Chemistry building B30
Contact: Chih-Jung Hsu, chihjung@sas.upenn.edu; Michael Heinrich, heinric@sas.upenn.edu
- Olympus IX71 inverted microscope stand
- Wide field fluorescence
- White light source: halogen lamp
- Fluorescence light source: mercury lamp
- Objectives: 60X water, and 60X oil (TIRF)
- Laser lines: 405, 488, 561, 1024nm
- Filter sets: Dapi, FITC, and Texas Red
- Acquisition software: HCImage
- Detector: HAMAMATSU EMCCD