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Ultraview Spinning Disk Confocal

Ultraview Spinning Disk Confocal (Burkhardt) –used for rapid fluorescence imaging of living cells.  Equipped for ratiometric imaging and photobleaching, this microscope is useful for analysis of protein-protein interactions and protein mobility using biophysical approaches such as Fluorescence Resonance Energy Transfer (FRET) and Fluorescence recovery after photobleaching (FRAP).

Ultraview Spinning Disk ConfocalScope location: ARC 815 
Contact: Fiona Clarke, 267-426-5523, clarkef@email.chop.edu

  • Zeiss Axiovert 200 Stand
  • Perkin-Elmer UltraVIEW CSU22 Scan head
  • White Light source: Halogen Lamp
  • Fluorescence Light source: Mercury Lamp
  • Objectives: 10X air, 20X LD air, 40X air, 40X oil, 63X oil, 100X oil
  • Laser Lines:
    • Argon 488nm and 514nm
    • Argon/Krypton 568nm
    • Diode laser 405nm, 440 nm and 640nm
  • Photokinesis unit for photobleaching (FRAP, FRET, photoactivation)
  • Acquisition Software: Improvision Volocity
  • Surrounded by Solent Scientific environmental chamber with heating capabilities for use in live-cell imaging
  • Hamamatsu ORCA ER camera
  • Piezo on objective