Other Perelman School of Medicine Affiliations
Pennsylvania Muscle Institute
Institute for Environmental Medicine

Degrees
B.S., Cornell University, 1966
Ph.D., Cornell University, 1971

Professional Affiliations
Biophysical Society

Research Description
Biological molecule motors transduce chemical energy into mechanical work, the fundamental mechanism underlying muscle contraction and other forms of Cell Motility. Recent advances in sample preparation, instrumentation, including infrared laser optical traps (laser tweezers) and data analysis now enable the measurement of mechanical properties on single motor molecules. We have constructed an enhanced version of the laser tweezers and have developed new protocols to quantitatively measure the mechanics of single molecules of myosin interacting with actin filaments.

Lung surfactant is a complex mixture of lipids and proteins which functions to lower the surface tension of the air-water interface of the alveolar hypophase. Lung epithelial type II cells regulate the alveolar surfactant pool size by both secreting the contents of lamellar bodies, the surfactant storage vesicle, into the alveolar space and also retrieving surfactant from the alveolar space. Our current research is aimed at identifying the lamellar body membrane components involved in organelle biogenesis and secretion.

Representative Publications
Litvinov R.I., Shuman H., Bennett J.S. and J.W. Weisel. Binding strength and activation state of single fibrinogen-integrin pairs on living cells. Proc Natl Acad Sci U S A. 99:7426-31. 2002.

Mulugeta S., Gray J.M., Notarfrancesco K.L., Gonzales L.W., Koval M., Feinstein S.I., Ballard P.L., Fisher A.B. and H. Shuman. Identification of lbm180 a Lamellar body limiting membrane protein of Alveolar type II cells as the ABC transporter protein, ABCA3. J Biol Chem. 277:22147-22155, 2002.

Click here for a full list of publications
(searches the National Library of Medicine's PubMed database.)