Introduction to Laser Capture Microdissection
Laser capture microdissection (LCM) has become an important
tool in biological research, permitting the isolation of single
cells, or group of cells, from a heterogeneous population of
cells; LCM is also sufficiently precise to isolate chromosomes.
LCM technique allows accurate profiling of cells, often obscured
in samples prepared by other methods, where heterogeneous cell
populations are unavoidably present.
Our core facility uses the PALM (Positioning Ablation Laser Micro
beam) Laser Catapult from Carl Zeiss, which combines LCM and
Laser Pressure Catapulting (LPC). In this non-contact isolation
method, single cells or groups of cells are catapulted into the
sample tube by photonic pressure force. Since LCM does not damage
the morphology of the collected cell or the tissue around it,
the slide may be stored or used for other purposes. Several different
cells populations could be collected from the same slide. LCM
can be performed on frozen and fixed tissue samples, including
blood smears, cytological preparations, cell cultures and aliquots
of solid tissue. LCM/LPC is compatible with various cytological
and immuno-staining techniques.
Once the cells of interest have been isolated, a variety of
analytical techniques can be applied, such as MALDI mass spectrometry,
two-dimensional polyacrylamide gel electrophoresis, Western blotting,
quantitative and RT PCR, DNA microarrays, or mutation analysis.
Combinations of these techniques make the analyses even more
To learn more about LCM: www.palm-microlaser.com
The core is located on the 7th Floor of the Leonard and Madlyn
Abramson Pediatric Research Center in room 706.
Neal A. Rubinstein, MD, PhD – Core Director
Olga Lozynska, MSc – To schedule an appointment or consultation,
please e-mail: firstname.lastname@example.org
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