Penn Medicine

Yale Goldman, M.D., Ph.D.

The light chain region of myosin tilts during step length changes and active force generation in skeletal muscle.

Irving M, Allen TStC, Sabido-David C, Craik JS, Brandmeier B, Kendrick-Jones J, Corrie JET, Trentham DR, Goldman YE

Nature 375:688-691, 1995.

Force generation and relative sliding between the myosin and actin filaments in muscle are thought to be caused by tilting of the head region of the myosin crossbridges between the filaments. Structural and spectroscopic experiments have demonstrated segmental flexibility of myosin in muscle, but have not shown a direct linkage between tilting of the myosin heads and either force generation or filament sliding. Here we use fluorescence polarization to detect changes in the orientation of the light-chain region of the head, the part most likely to tilt, and synchronized head movements by imposing rapid length steps. We found that the light-chain region of the myosin head tilts both during the imposed filament sliding and during the subsequent quick force recovery that is thought to signal the elementary force-generating event.

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Perelman School of Medicine University of Pennsylvania
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