Penn Medicine

Yale Goldman, M.D., Ph.D.

Sun Y, McKenna JD, Murray JM, Ostap EM, Goldman YE.

Parallax: high accuracy three-dimensional single molecule tracking using split images.

Nano Lett. 9:2676-2682. 2009.

Three-dimensional (3D) tracking can provide valuable biological insights that are missing in conventional microscopy. Here we developed a single molecule 3D tracking microscopy technique, named Parallax, with high localization precision and temporal resolution. We demonstrated its capabilities by studying the 3D trafficking of glucose-transporter-4 containing vesicles in living adipocytes as well as the walking path of single myosin VI molecules along actin filaments.

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Pennsylvania Muscle Institute
Perelman School of Medicine University of Pennsylvania
Director: E. Michael Ostap, Ph.D.

700A Clinical Research Building Philadelphia, PA 19104-6085 Phone: (215) 573-9758 Fax: (215) 898-2653