Parallax: high accuracy three-dimensional
single molecule tracking using split images.
Nano Lett. 9:2676-2682. 2009.
Three-dimensional (3D) tracking can provide
valuable biological insights that are missing in conventional
microscopy. Here we developed a single molecule 3D tracking
microscopy technique, named Parallax, with high localization
precision and temporal resolution. We demonstrated its capabilities
by studying the 3D trafficking of glucose-transporter-4 containing
vesicles in living adipocytes as well as the walking path
of single myosin VI molecules along actin filaments.