| Projects > 2011 > Caitlyn Riehl

Improving Accuracy in Imaging and Treating Tumors

Caitlyn Riehl
mentored by Dr. Gary Kao.

Goals and Hypotheses
:: Use tattooing in a safe and effective manner.
:: Utilize the imaging techniques we have to more accurately treat the mice.
:: Ionizing radiation will cause collapse, less perfusion and shrinkage in the vasculature of tumors.
:: U251-GFP-LUC (non-HIC) and U251-GFP-LUC-HIC cell lines, although originated from the same cell lines, have very different properties and will behave differently within the mouse model.






Techniques
:: Cell Culture.
:: Subcutaneous Tumor Injection.
:: Bioluminescent Imaging.
:: CT Scans.
:: Fluorescent Imaging using a PEGylated Dye.
:: Measuring Tumors using Electronic Calipers.
:: Small Animal Tattooing.
:: Immunohistochemistry.
:: Use of the SARRP.

Conclusions
:: Tattooing is a safe and durable way to identify nude mice and mark treatment sites.
:: The U251-GFP-LUC-HIC cell line has bioluminescent signal, growth rates and vascular contrast uptake patterns that seem surprisingly distinct from its parental cell line.
:: Imaging using CT contrast in mice is very possible and can help in giving more effective treatment, but needs to be explored more.
:: CD31 expression/vascular marker patterns may differ between tumors grown from different cell lines and possibly after irradiation.
:: These techniques collectively may improve the imaging and treatment of tumors grown in mice.