| Projects > 2011 > James Ruggero

In Vivo Profiling of Hypoxic miRNA in 9L Gliosarcoma Tumors Using the Hypoxia Marker EF5 and Laser-Capture Microdissection

James Ruggero
mentored by Dr. Amit Maity and Dr. Costas Koumenis

Goal
Create a miRNA profile, that can be used in conjuction with a previously generated messenger RNA profile, to study the mechanisms that protect cancer cells in a hypoxic state, as well as allow them to proliferate, invade and metastasize.







Techniques
:: Laser Capture Microdissection.
:: NanoDrop Analysis for RNA Concentration.
:: RNA Pico Card Analysis for RNA Degredation
:: In Vivo Profiling using Taqman® Array miRNA cars for real time PCR.

Conclusions
We found that a previously known miRNA in the hypoxic response, miR-210, was upregulated, confirming the utility of the Taqman® Array. Other miRNAs have been found using this array but require further analysis to evaluate their significance.