| Projects > 2011 > Shire Beach

Creating Stable Cell Lines of Inducible ATF4 and GCN2 Knockdowns

Shire Beach
mentored by Dr. Costas Koumenis

Goal
To create stable cell lines of inducible ATF4 and GCN2 knockdowns. These knockdowns will be used in sutdies which aim to support the hypothesis that ATF4 and GCN2 promote tumor growth.







Techniques
:: Maxi/Minipreps.
:: Tissue Culture and Transfections.
:: Western Blot.
:: Fluorescence Microscopy.
:: FACS.

Conclusions
The inducible knockdown constructs were transfected in to several cell lines (HT1080, B16F10) and some stable clones were obtained. Further analysis however is required to complete the goals of this project.