Marcia Holsbach Beltrame

Marcia Holsbach Beltrame, Ph.D.

Postdoctoral Researcher

Education

Ph.D.

Genetics, Universidade Federal do Paraná, Brazil, 2008-2012                                  
Advisors: Prof. Maria Luiza Petzl-Erler and MD. PhD Arthur A. Simen (Yale University)
Title: “Evolutive aspects and functional impact of CD80 and CD86 gene polymorphisms”

Masters

Genetics, Universidade Federal do Paraná, Brazil, 2007-2008                          
Advisor: Prof. Maria Luiza Petzl-Erler
Title: “Polymorphisms of CD80 and CD86 genes in populations of various ancestries”

Bachelors

Biology, Universidade Federal do Paraná, Brazil, 2002-2006                         
Advisor: Prof. Maria Luiza Petzl-Erler
Title: “Gene diversity of CD80 and CD86 in the population of Curitiba, Parana, Brazil.”

Research Experience

2013 - 2014

Postdoctoral Fellow                                                                                    
Hospital de Clínicas da Universidade Federal do Paraná, Brazil                   
Supervisor: Prof. Dr. Iara de Messias Reason

2012 - 2013

Postdoctoral Fellow                                                                        
Federal University of Paraná, Brazil             
Research under the direction of Prof. Dr. Maria Luiza Petzl-Erler
Teaching under the direction of Prof. Juarez Gabardo and Prof. Dr. Maria Luiza Petzl-Erler

Scholarships

2013 - 2014

PRODOC (Programa de Apoio a Projetos Institucionais com a Participação de Recém-Doutores).
Title: “HBV and HCV coinfection prevalence in HIV+ patients from Curitiba: association with genetic and epidemiological factors” (Postdoctoral fellowship)

2012 - 2013

REUNI (Programa de Apoio a Planos de Reestruturação e Expansão das Universidades Federais).
Project: “Methylation and gene expression patterns in patients with endemic pemphigus foliaceus and functional analysis of promoter polymorphisms” (Postdoctoral fellowship)

2010 - 2011

CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)
Project: “Functional Analysis of CD80 and CD86 promoter polymorphisms” (Sandwich PhD scholarship – for studying abroad)

2008 - 2012

CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)
Project: “Evolutive aspects and functional impact of CD80 and CD86 gene polymorphisms” (PhD scholarship)

2007 - 2008

CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)
Project: “Polymorphisms of CD80 and CD86 genes in populations of various ancestries” (Masters Scholarship)

2005 - 2006

PIBIC/CNPQ (Conselho Nacional de Desenvolvimento Científico e Tecnológico)
Project: “Human genome variability: from normal variation to disease” (Undergraduate internship)

2004 - 2005

PIBIC/CNPQ (Conselho Nacional de Desenvolvimento Científico e Tecnológico)
Project: “Genes of the imune system: variability in populations” (Undergraduate internship)

Peer-reviewed Publications

  • Evans-Osses, I; Mojoli, A; Beltrame, MH; da Costa, DE; da Rocha, WD; Velavan, TP; de Messias-Reason, I; Ramirez, MI. Differential ability to resist to complement lysis and invade host cells mediated by MBL in R4 and 860 strains of Trypanosoma cruzi. FEBS Letters, v. 588, n. 6, p. 956-961, 2014.

    Beltrame, MH; Pincerati, MR; Dalla-Costa, R; Wassem, R; Köhler, KF; Chautard-Freire-Maia, EA; Tsuneto, LT; Petzl-Erler, ML. CD80 and CD86 polymorphisms in populations of various ancestries: 5 new CD80 promoter alleles. Human Immunology, v. 73, p. 111-117, 2012.

    Torres, MC; Beltrame, MH; Santos, ICR; PICHETH, G; PETZL-ERLER, ML; Pedrosa, FO; Steffens, MBR; de Souza, EM. Polymorphisms of the promoter and exon 3 of the receptor for advanced glycation end products (RAGE) in Euro- and Afro-Brazilians. International Journal of Immunogenetics (Print), v. 39, p. 155-160, 2011.

    Kelmendi B, Holsbach-Beltrame M, McIntosh AM, Hilt L, George ED, Kitchen RR, Carlyle BC, Pittenger C, Coric V, Nolen-Hoeksema S, Sanacora G, Simen AA. Association of polymorphisms in HCN4 with mood disorders and obsessive compulsive disorder. Neuroscience Letters. 2011 Jun 8;496(3):195-9.

    Dalla-Costa, R; Pincerati, MR; Beltrame, MH; Malheiros, D; Petzl-Erler, ML. Polymorphisms in the 2q33 and 3q21 chromosome regions including T-cell coreceptor and ligand genes may influence susceptibility to pemphigus foliaceus. Human Immunology, v. 71, p. 809-817, 2010.

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Conference Presentations

Beltrame, MH; Simen, AA; Dalla-Costa R; Carlyle, BC; Gabardo, J; Shaw, AC; Petzl-Erler, ML. Polymorphisms in the promoter region of CD80 and CD86 alter gene expression. 58º Brazilian Genetics Conference, September 2012, Foz do Iguaçu, Paraná. (poster presentation)

Beltrame, MH; Dalla-Costa, R; Simen, AA; Petzl-Erle, ML. Functional Analysis of the CD80 Promoter. 25th European Immunogenetics and Histocompatibility Conference, 2011, Prague, Czech Republic. EFI 2011 - Tissue Antigens, 2011. v. 77. p. 382-382. (oral presentation)

Beltrame, MH; Dalla-Costa, R; Simen, AA; Petzl-Erle, ML. Polymorphisms in the promoter region of the CD80 gene alter gene expression in vitro. 57º Brazilian Genetics Conference, September 2011, Águas de Lindóia, São Paulo. (poster presentation)

Beltrame, MH; Pincerati, MR; Dalla-Costa, R; Tsnuet, LT; Chautard-Freire-Maia, EA; Petzl-Erle, ML. Evolutionary Insights into CD80 and CD86 gene polymorphisms. 55º Brazilian Genetics Conference, September 2009, Águas de Lindóia, São Paulo. (poster presentation)

Beltrame, MH; Pincerati, MR; Dalla-Costa, R; Tsnueto, LT; Chautard-Freire-Maia, EA; Petzl-ErleR, ML. CD80 and CD86 gene polymorphisms in populations of various ancestries. 23rd European Immunogenetics and Histocompatibility Conference, 2009, Ulm, Alemanha. Tissue Antigens, 2009. v. 73. p. 479-479. (poster presentation)

Beltrame, MH; Pincerati, MR; Dalla-Costa, R; Petzl-Erle, ML. Variability of SNP 1057 (G>A) in CD86 gene in the population of Curitiba. 54º Brazilian Genetics Conference, September 2008, Salvador, Bahia. (poster presentation)

Beltrame, MH; Pincerati, MR; Petzl-Erle, ML. Diversity of CD80 gene in the population of Curitiba. 53º Brazilian Genetics Conference, September 2007, Águas de Lindóia, São Paulo. (poster presentation)

Beltrame, MH; Oliveira, LA; Petzl-Erle, ML. Diversity of microsatelites in the Major Histocompatibility Complex (MHC) in the population of Curitiba. 52º Brazilian Genetics Conference, 2006, Foz do Iguaçu, Paraná. (poster presentation)

Beltrame, MH; OliveiraA, LA; Petzl-Erle, ML. Diversity of microsatelites in the Major Histocompatibility Complex (MHC) in the population of Curitiba. 51º Brazilian Genetics Conference, 2005, Águas de Lindóia, São Paulo. (poster presentation)

Courses Given

Beltrame, MH.  Functional analysis of promoter polymorphisms. V Genetics winter course. 4,5 hours. July 2012.

Beltrame, MH; Dalla-Costa, R. Sequencing. II Genetics winter course. 3 hours. July 2009.

Beltrame, MH; Augusto, DG. Molecular techniques applied to research. II Genetics winter course. 2009.

Beltrame, MH. Principles, applications and development of molecular techniques PCR SSP, PCR SSOP e PCR RFLP. I Genetics winter course. 3 hours. July 2008.

Research awards:

Third place in the 13º Undergraduate Students Meeting -UFPR, Coordenadoria de Iniciação Científica e Integração Acadêmica - UFPR. 2005, Project: “Genes of the immune system: variability in populations”

Reviewer

2013 – International Journal of Immunogenetics
2012 – Revista Brasileira de Biociências

Professional Societies

2007 - present - The Brazilian Society of Genetics

Courses (complementary education):

2011 – Extraordinary junk: cisregulatory elements. (3h). Brazilian Society of Genetics.
2010 – Immunosenescence & Diagnostic Immunology. (6h). Sociedade Brasileira de Imunologia.
2009 – Genetic methods for infering positive selection. (3h). Brazilian Society of Genetics.
2008 – Bioinformatics. (3h). Brazilian Society of Genetics.
2008 – Statistical analysis for real time PCR. (4h). Eppendorf Brasil.
2008 – Utilization of SNPs in Forensic Genetics. (3h). Brazilian Society of Genetics.
2007 – Epigenetics: new directions in human genetics. (3h). Brazilian Society of Genetics.
2006 – Genetic variation in natural populations. (10h). Centro Acadêmico dos Estudantes de Biologia.
2006 – Evolution and Molecular Phylogeny. (3h). Brazilian Society of Genetics.
2006 – Mechanisms of Mutagenesis and DNA repair. (3h). Brazilian Society of Genetics.
2005 – Aplications of SNPs in the study of complex diseases. (3h). Brazilian Society of Genetics.
2005 – Tools for the study of population structure. (3h). Brazilian Society of Genetics.
2005 – Forensic molecular biology. (10h). Diretório Acadêmico dos Estudantes de Bioprocessos e Biotecnologia.
2005 – Practice on blood draw. (60h). Hospital de Clínicas da Universidade Federal do Paraná.

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