Wand Lab - University of Pennsylvania

The Wand "How To" Resource
Reverse micelles High Pressure Software NMR Pulse Sequences Vectors		Reverse micelles Reverse micelle encapsulation is a useful strategy in a range of solution NMR applications. It is however a complex technology touching on physical chemistry, protein chemistry and a host of NMR issues. Here we are trying provide practical tips to enable investigators to find encapsulation conditions for their protein of interest, how to prepare such solutions in low viscosity fluids and solutions to the various NMR issues that solutions of encapsulated proteins often present. We no longer manufacture in-house the apparatus used in these types of studies. More cost effect solutions are available commerically from Daedalus Innovations. For more detailed information click here. Disclosure: A.J.W. declares a competing financial interest as a Member of Daedalus Innovations, LLC.
		High Pressure High pressure NMR has existed in various forms since the very beginning of the NMR field. The original autoclave design, where the entire NMR probe was put under pressure, has proven unworkable in the context of state-of-the-art solution NMR spectroscopy. We developed a novel tube sealing method that allows for high pressure NMR tubes to be used in a state-of-the-art cryoprobe. We no longer manufacture the tubes but do provide helpful hints on the use of a commerical product from Daedalus Innovations. For more detailed information click here. Disclosure: A.J.W. declares a competing financial interest as a Member of Daedalus Innovations, LLC.
		Software We have recently upgraded our Relxn2A software package for analysis of NMR relaxation data using the model-free formalism. Originally written by Marty Dellwo & Josh Wand in 1989, the program was upgraded significantly by Andrew Lee in 1997-2000. Recently Vignesh Kasinath and Vonni Moorman have greatly extended its capabilities. The package is provided as a binary executable. Radial sampling is proving to provide significant advantages for NMR data collections with respect to aquisition time, optimized signal-to-noise and serial sampling of hydrogen exchange in large proteins. Proper processing of radial sampling is best undertaken with true multidimensional (i.e. simulataneous) Fourier transformation rather than sequential one-dimensional FFTs. Written largely by John Gledhill, we are releasing a processing package ("Al") to academics and non-profits for non-commerical use. The program also processes conventionally aquired NMR data. The program is provided as a binary executable. For more detailed information, click here: http://130.91.181.107:8280/SitePages/Home.aspx
		NMR Pulse Sequences We have recently converted our spectrometers to Bruker Avance III consoles and cryoprobes. As part of this transition, we have optimized many of the Bruker pulse sequences and corrected significant errors in a few. In addition, we have coded the deuterium and carbon relaxation experiments for a variety of labeling contexts. Though they have been extensively tested, these pulse sequences are provided without any warranty or guarantee. Safe implementation on a given spectrometer and cryoprobe is the responsibility of the user. We simply do not have the resources to provide individual support. For information on the pulse sequences that are available, click here.
		Vectors We have constructed a number of expression vectors and developed associated protocols as part of our federally funded research effort. We are happy to provide them to academics and non-profit institutions for non-commercial use. A material-transfer-agreement must be completed by the responsible principal investigator and an express mail billing account is required. For a list of currently available vectors click here.