Anna Pastuszko, Ph.D.

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Research Associate Professor of Biochemistry and Biophysics
Department: Biochemistry and Biophysics

Contact information
901 Stellar-Chance Building
University of Pennsylvania
Philadelphia, PA 19104-6059
Office: 2158986382
Lab: 215-573-1129
M.S. (Microbiology)
Warsaw University, Poland , 1970.
Ph.D. (Neurochemistry)
Medical Research Center, Polish Academy of Science, 1977.
Post Doc (Dr. Habilitation in Medical Science, Neuroscience)
Polish Academy of Sciences., 1984.
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Description of Research Expertise

My laboratory has been interested in hypoxic/ischemic injury to the brain of the newborn for many years. The newborn piglet model is particularly well suited for studies on clinically related problems since the level of maturation and physical size are similar to those of the human newborn. We have focused on the early biochemical and molecular biological events following hypoxic ischemic insult, typically within the first 6-12 hrs. It is considered that these early events, including changes in gene expression and activation of signaling cascades, are good predictors of the level of injury and provide insight into the mechanism(s) of the functional losses that will follow. Currently we are focused on apoptotic cell death, an important mechanism for injury in the newborn brain, and inflammation, which can greatly exacerbate the level of injury through a variety of mechanisms. As we gain knowledge of the mechanisms underlying the functional injury, we are studying the efficacy of drugs that target the putative injury producing signaling pathways, quantifying their ability to decrease the signaling related to tissue injury and to enhance the signaling associated with protection of the brain. An important part of my research program is a close collaboration with clinical colleagues in order to optimally focus our research efforts and to facititate translation of our findings into the clinics. This goal is also reflected in my choice of research models, which include cardiopulmonary bypass with deep hypothermic circulatory arrest, repetitive apnea, and periods of hypoxia/ischemia, all using conditions closely approximating those seen in the clinics.

Selected Publications

Pastuszko P, Schears GJ, Kubin J, Wilson DF, Pastuszko A. : Granulocyte colony-stimulating factor significantly decreases density of hippocampal caspase 3-positive nuclei, thus ameliorating apoptosis-mediated damage, in a model of ischaemic neonatal brain injury. Interact Cardiovasc Thorac Surg 25(4): 600-605, 2017.

Pastuszko Peter, Schears Gregory J, Greeley William J, Kubin Joanna, Wilson David F, Pastuszko Anna: Granulocyte colony stimulating factor reduces brain injury in a cardiopulmonary bypass-circulatory arrest model of ischemia in a newborn piglet. Neurochemical research 39(11): 2085-92, Nov 2014.

Pastuszko, P., Schears, GJ. Kubin, J. , Greeley, W J., Nadkarni, V., Wilson, D.F. Pastuszko, A.: Granulocyte-colony stimulating factor suppresses early inflammatory response of striatum in a cardiopulmonary bypass-circulatory arrest model of ischemic brain injury in newborn piglets. World Journal of Cardiovascular Diseases 3: 197-205, April 2013.

Pastuszko, Peter. Schears, Gregory J. Pirzadeh, Afsaneh. Kubin, Joanna. Greeley, William J. Wilson, David F. Pastuszko, Anna.: Effect of granulocyte-colony stimulating factor on expression of selected proteins involved in regulation of apoptosis in the brain of newborn piglets after cardiopulmonary bypass and deep hypothermic circulatory arrest. Journal of Thoracic & Cardiovascular Surgery 143(6): 1436-42, Jun 2012.

Wilson, David F. Vinogradov, Sergei A. Schears, Gregory J. Esipova, Tatiana V. Pastuszko, Anna.: Monitoring cardiopulmonary function and progression toward shock: oxygen micro-sensor for peripheral tissue. Advances in Experimental Medicine & Biology 737: 221-7, 2012.

Mammen, A. Kubin, J. Greeley, W J. Schears, G J. Pastuszko, P. F Wilson, D. Pastuszko, A.: Effect of hypoxia on expression of selected proteins involved in regulation of apoptotic activity in striatum of newborn piglets. Neurochemical Research 36(5): 746-53, May 2011.

Pirzadeh, Afsaneh. Schears, Gregory. Pastuszko, Peter. Liu, Huiping. Kubin, Joanna. Reade, Erin. Mendoza-Paredes, Alberto. Greeley, William. Nadkarni, Vinay. Wilson, David F. Pastuszko, Anna.: Effect of deep hypothermic circulatory arrest followed by low-flow cardiopulmonary bypass on brain metabolism in newborn piglets: comparison of pH-stat and alpha-stat management. Pediatric Critical Care Medicine 12(2): e79-86, Mar 2011.

Pirzadeh, A. Mammen, A. Kubin, J. Reade, E. Liu, H. Mendoza, A. Greeley, W J. Wilson, D F. Pastuszko, A.: Early regional response of apoptotic activity in newborn piglet brain following hypoxia and ischemia. Neurochemical Research 36(1): 83-92, Jan 2011.

Wilson, David F. Finikova, Olga S. Lebedev, Artem Y. Apreleva, Sophia. Pastuszko, Anna. Lee, William M F. Vinogradov, Sergei A.: Measuring oxygen in living tissue: intravascular, interstitial, and "tissue" oxygen measurements. Advances in Experimental Medicine & Biology 701: 53-9, 2011.

Pastuszko, Peter. Pirzadeh, Afsaneh. Reade, Erin. Kubin, Joanna. Mendoza, Alberto. Schears, Gregory J. Greeley, William J. Pastuszko, Anna.: The effect of hypothermia on neuronal viability following cardiopulmonary bypass and circulatory arrest in newborn piglets. European Journal of Cardio-Thoracic Surgery 35(4): 577-81; discussion 581, Apr 2009.

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Last updated: 01/30/2018
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