Changhong Li, MD, Ph.D

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Research Assistant Professor of Pediatrics
Department: Pediatrics

Contact information
802B, ARC
3516 Civic Center Blvd.
Philadelphia, PA 19104
Office: (215)5905380
Fax: (215)5901605
Education:
B.A. (Medicine)
ChengDe Medical Collage, CHINA, 1989.
MD, PhD (Internal Medicine, Endocrinology)
Beijing Medical University, China, 1996.
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Description of Research Expertise

Research Interest:

My research mainly focused on fuel metabolism in islets and regulation of insulin secretion in congenital hyperinsulinism (HI), a group of disorders that cause hypoglycemia in infants and children. My work has involved characterizing most of the known disease models of HI, including glutamate dehydrogenase transgenic mice, sulfonylurea receptor 1 knockout mice, and short-chain 3-hydroxy fatty acyl-CoA dehydrogenase knockout mice. Recently, I have extended these studies of animal models to examine the role of amino acids in regulation of insulin and glucagon secretion by islets from normal and type 2 diabetic humans.

Techniques:

Islets function evaluation: 1) Islets isolation and function studies by batch incubation in 96 well “high-throughput” format and by islets perifusions. 2) Measurement of intracellular calcium in perifused whole islets or dispersed islet cell by duel wave length florescence microscopy.
Fuel Metabolism: 1) Stable isotope tracing: 13C and 15N tracing in isolated islets to study metabolic fluxes from glucose or amino acid; 2)ATP and ATP/ADP ratio measurement to evaluate fuel metabolism.
Gene expression: study gene expression by qPCR in whole islets or in purified beta-cell by cell sorting.

Selected Publications

Shekhar Srivastava, Zhai Li, Irfana Soomro, Ying Sun, Jianhui Wang, Li Bao, William A. Coetzee, Charles A. Stanley, Chonghong Li and Edward Y. Skolnik: Regulation of KATP Channel Trafficking in Pancreatic β Cells by Protein Histidine Phosphorylation. Diabetes Page: online first, Feb 2018.

Ming Lu, Changhong Li. : Nutrient sensing in pancreatic islets: lessons from congenital hyperinsulinism and monogenic diabetes. Annals of the New York Academy of Sciences 1411(1): 65-82, Jan 2018.

Cetewayo S. Rashid, Yu-Chin Lien, Amita Bansal, Lane J. Jaeckle-Santos, Changhong Li, Kyoung-Jae Won, and Rebecca A. Simmons : Transcriptomic analysis reveals novel mechanisms mediating islet dysfunction in the intrauterine growth restricted (IUGR) rat. Endocrinology 159(2): 1035-1049, Fed 2018.

Mary Barrosse‐Antle, Chang Su, Pan Chen, Kara E Boodhansingh, Thomas J Smith, Charles A Stanley, Diva D De León, Changhong Li: A severe case of hyperinsulinism due to hemizygous activating mutation of glutamate dehydrogenase. Pediatric Diabetes 18(8): 911-916, December 2017.

Shan He,Yongnian Liu, Lijun Meng, Hongxing Sun, Ying Wang, Yun Ji, Janaki Purushe, Pan Chen, Changhong Li, Jozef Madzo, Jean-Pierre Issa, Jonathan Soboloff, Ran Reshef, Bethany Moore, Luca Gattinoni, Yi Zhang. : Ezh2 phosphorylation state determines its capacity to maintain CD8+ T memory precursors for antitumor immunity. Nat Commun. 8(1): 2125:1-14, December 2017.

Ge Li, Linxin Xu, Yanglu Zhao, Lujiao Li, Junling Fu, Qian Zhang, Naishi Li, Xinhua Xiao, Changhong Li, Jie Mi, Shan Gao, Ming Li. : Leptin-adiponectin imbalance as a marker of metabolic syndrome among Chinese children and adolescents: The BCAMS study. PLoS ONE 12(10): e0186222, October 2017.

Nicolai M. Doliba, Qin Liu, Changhong Li, Chengyang Liu, Ali Naji, Franz M. Matschinsky: Inhibition of Cholinergic Potentiation of Insulin Secretion from Pancreatic Islets by Chronic Elevation of Glucose and Fatty Acids: Protection by Casein Kinase 2 Inhibitor. Molecular Metabolism 6(10): 1240-1253, October 2017.

Hong Q. Smith, Changhong Li, Charles A. Stanley, Thomas James Smith: Glutamate Dehydrogenase, a Complex Enzyme at a Crucial Metabolic Branch Point. Neurochem Res. October 2017.

Amita Bansal, Cetewayo Rashid, Frances Xin, Changhong Li, Erzsebet Polyak, Anna Duemler, Tom van der Meer, Martha Stefaniak, Sana Wajid, Nicolai Doliba, Marisa S. Bartolomei, and Rebecca A. Simmons. : Sex-specific Multigenerational Effects of Maternal Bisphenol A Exposure on Offspring Pancreatic Islets. Environmental Health Perspectives 125(9): 97022 1-18, September 2017.

Changhong Li, Amanda M. Ackermann, Kara E. Boodhansingh, Tricia R. Bhatti, Chengyang Liu, Jonathan Schug, Nicolai Doliba, Bing Han, Karen E. Cosgrove, Indraneel Banerjee, Franz M. Matschinsky, Itzhak Nissim, Klaus H. Kaestner, Ali Naji, N. Scott Adzick, Mark J. Dunne, Charles A. Stanley and Diva D. De León: Functional and Metabolomic Consequences of ATP-dependent Potassium Channel Inactivation in Human Islets. Diabetes 66(7): 1901-1913, July 2017.

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Last updated: 04/12/2018
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