Jason Adam Mills, PhD

faculty photo
Research Assistant Professor of Ophthalmology
Department: Ophthalmology

Contact information
422 Curie Blvd, Stellar Chance Laboratories, Suite 309B
Philadephia, Pa 19104
Office: 215-746-3482
Education:
BS (Pre-veterinary Medicine)
University of Delaware, 1999.
MS (Animal Science)
University of Nebraska, 2002.
PhD (Stem Cell and Molecular Biology)
University of Delaware, 2008.
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Description of Research Expertise

Jason A. Mills, PhD is the Director of iPSC Facility at the Center for Advanced Retinal and Ocular Therapeutics (CAROT).

iPSC modeling & mechanisms of retinal degeneration

Our team is working to increase understanding of molecular and cellular etiologies associated with common and rare blinding mutations. To achieve this goal, we utilize unaffected normal sighted controls and patient-specific pluripotent stem cells (iPSCs) harboring pathogenic mutations. By characterizing early developmental stages of retinal tissue generation, we hope to identify mechanisms that may lead to a better understanding of disease progression and new targets for therapeutic interventions.

iPS cells offer a potentially unlimited source of numerous cell types for drug screening and targeted cell-based therapies.

Our expertise and services include:
· Generation of induced pluripotent stem cells using transgene-free approaches such as Sendai virus or episomal vectors.
o Somatic tissues: fibroblast, PBMCs, lymphoblastoid cells (LCLs)
· Protocols available for generation of retinal subtypes
o Retinal progenitor cells (RPCs)
o Retinal pigmented epithelium (RPEs)
o Retinal ganglion cells (RGCs)
o Photoreceptors
· Gene targeted iPSC lines (Crispr/Cas9, Safe-harbor, Lentivirus)
· Expertise on pilot iPSC endeavors
· Experience with IND-enabling studies using iPSCs and derivatives.

Additional know-how with projects include studying development and regulation of blood lineages (RBCs, megakaryocytes, myeloid cells), cardiac (cardiomyocytes), and neural cells (forebrain pyramidal neurons).
Lab Personnel
Carrie Merkel: iPSC Core Technical Director merkelc@mail.med.upenn.edu
Pamela Herrera: Research Specialist pherr@mail.med.upenn.edu
Lindsey Weed: PhD Candidate weed@mail.med.upenn.edu
Thu Duong: PhD Candidate thuduong@mail.med.upenn.edu
Pablo Golac: Undergraduate Researcher

Selected Publications

Mills, JA, Herrera, PS, Kaur, M, Leo, L, McEldrew, D, Tintos-Hernandez, JA, Rajagopalan, R., Alyssa Gagne, A., Zhang, Z., Ortiz-Gonzalez, X., Krantz, ID. : NIPBL+/- haploinsufficiency reveals a constellation of transcriptome disruptions in the pluripotent and cardiac states. Scientific Reports 8(1): 1056, 2018.

Duong, T., Vasireddy, V, Ramachandran, P., Herrera, P, Leo, L., Merkel, C., Bennett, J., Mills, JA. : Use of Induced Pluripotent Stem Cell models to probe the pathogenesis of Choroideremia and to develop a potential treatment. Stem Cell Research 27: 140-150, 2018.

Song, JY, Aravand, P., Nikonov, S., Leo, L., Lyubarsky, A., Bennicelli, J.L, Pan, J., Wei, Z., Shpylchak, I., Herrera, P., Bennett, D.J., Commins, N., Maguire, A.M., Pham, J., den Hollander, A.I., Cremers, F. P.M., Koenekoop R., Roepman, R., Patsy Nishina, Zhou, S., Pan, W., Ying, G-S., Aleman, T.S., de Melo, J., McNamara, I., Sun, J., Mills, JA., Bennett, J.: Proof-of-concept for gene augmentation therapy in a mouse model of a blinding ciliopathy; Relevance to Human LCA5. Molecular Therapy 26(6): 1581-1593, 2018.

Maguire, J.A., Gagne, A., Mills, J.A., Gadue, P., & French, D.L. : Generation of human control iPS cell line CHOPWT9 from healthly adult peripheral blood mononuclear cells. Stem Cell Research 16(1): 14-16, 2016.

Maguire, J.A., Lu, L., Mills, J.A., Sullivan, L.M., Gagne, A., Gadue, P., & French, D.L. : Generation of hermansky pudlak syndrome type 1 (HPS2) induced pluripotent stem cells (iPSCs). Stem Cell Research 16(2): 233-235, 2016.

Mills Jason A, Hudock Kristin M, Sullivan Spencer K, Herrera Pamela, Sullivan Lisa M, Gadue Paul, French Deborah L: Generation of poikiloderma with neutropenia (PN) induced pluripotent stem cells (iPSCs). Stem cell research 15(3): 595-7, Nov 2015.

Ying Lei, Mills Jason A, French Deborah L, Gadue Paul: OCT4 Coordinates with WNT Signaling to Pre-pattern Chromatin at the SOX17 Locus during Human ES Cell Differentiation into Definitive Endoderm. Stem cell reports 5(4): 490-8, Oct 2015.

Wilson Andrew A, Ying Lei, Liesa Marc, Segeritz Charis-Patricia, Mills Jason A, Shen Steven S, Jean Jyhchang, Lonza Geordie C, Liberti Derek C, Lang Alex H, Nazaire Jean, Gower Adam C, Müeller Franz-Josef, Mehta Pankaj, Ordóñez Adriana, Lomas David A, Vallier Ludovic, Murphy George J, Mostoslavsky Gustavo, Spira Avrum, Shirihai Orian S, Ramirez Maria I, Gadue Paul, Kotton Darrell N: Emergence of a stage-dependent human liver disease signature with directed differentiation of alpha-1 antitrypsin-deficient iPS cells. Stem cell reports 4(5): 873-85, May 2015.

Antony-Debré Iléana, Manchev Vladimir T, Balayn Nathalie, Bluteau Dominique, Tomowiak Cécile, Legrand Céline, Langlois Thierry, Bawa Olivia, Tosca Lucie, Tachdjian Gérard, Leheup Bruno, Debili Najet, Plo Isabelle, Mills Jason A, French Deborah L, Weiss Mitchell J, Solary Eric, Favier Remi, Vainchenker William, Raslova Hana: Level of RUNX1 activity is critical for leukemic predisposition but not for thrombocytopenia. Blood 125(6): 930-40, Feb 2015.

Ge Jingping, Apicella Marisa, Mills Jason A, Garçon Loïc, French Deborah L, Weiss Mitchell J, Bessler Monica, Mason Philip J: Dysregulation of the Transforming Growth Factor β Pathway in Induced Pluripotent Stem Cells Generated from Patients with Diamond Blackfan Anemia. PloS one 10(8): e0134878, 2015.

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Last updated: 12/12/2018
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