Jason Adam Mills, PhD

faculty photo
Research Assistant Professor of Ophthalmology
Department: Ophthalmology

Contact information
Stellar Chance Laboratories, Suite 310
422 Curie Blvd
Philadephia, Pa 19104
Office: 215-746-3482
Education:
BS (Pre-veterinary Medicine)
University of Delaware, 1999.
MS (Animal Science)
University of Nebraska, 2002.
PhD (Stem Cell and Molecular Biology)
University of Delaware, 2008.
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Description of Research Expertise

Jason A. Mills, PhD is the Director of iPSC Core at the Center for Advanced Retinal and Ocular Therapeutics (CAROT).

Dr. Mills establishes in vitro models for inherited developmental and blinding disorders using induced pluripotent stem cells (iPSCs), and studies how dysregulated downstream genes “targets/effectors” are related to clinical manifestations.

The CAROT team is working to better understand the molecular and cellular etiologies associated with common and rare blinding mutations. To achieve this goal, they are using patient-specific pluripotent stem cells harboring pathogenic mutations and performing in vitro differentiation assays directed toward retinal cell types (RPE, retinal progenitors, photoreceptors). By characterizing early developmental stages of retinal tissue generation, they hope to identify mechanisms that may lead to a better understanding of disease progression and new targets for therapeutic interventions.

The CAROT members are dedicated to bridging the gap between the benchtop to bedside for treatment of blinding disorders.

The center has extensive experience in: (1) human ESC/iPSC maintenance and differentiation, (2) generation of iPSCs, (3) animal models for transplantation and diseases, (4) molecular biology, (5) genetics,
(6) epigenetics, and (7) genome editing.

Additional research projects include studying Cornelia de Lange Syndrome (CdLS) to better understand how mutations in the cohesin complex and associated proteins affect transcriptional and epigenetic regulation in blood, cardiac and neurological cell types.

Lab Personnel
Carrie Merkel: iPSC Core Technical Director merkelc@mail.med.upenn.edu
Pamela Herrera: Research Specialist pherr@mail.med.upenn.edu
Lindsey Weed: PhD Candidate weed@mail.med.upenn.edu
Thu Duong: PhD Candidate thuduong@mail.med.upenn.edu
Pablo Golac: Undergraduate Researcher

Selected Publications

Mills Jason A, Hudock Kristin M, Sullivan Spencer K, Herrera Pamela, Sullivan Lisa M, Gadue Paul, French Deborah L: Generation of poikiloderma with neutropenia (PN) induced pluripotent stem cells (iPSCs). Stem cell research 15(3): 595-7, Nov 2015.

Ying Lei, Mills Jason A, French Deborah L, Gadue Paul: OCT4 Coordinates with WNT Signaling to Pre-pattern Chromatin at the SOX17 Locus during Human ES Cell Differentiation into Definitive Endoderm. Stem cell reports 5(4): 490-8, Oct 2015.

Wilson Andrew A, Ying Lei, Liesa Marc, Segeritz Charis-Patricia, Mills Jason A, Shen Steven S, Jean Jyhchang, Lonza Geordie C, Liberti Derek C, Lang Alex H, Nazaire Jean, Gower Adam C, Müeller Franz-Josef, Mehta Pankaj, Ordóñez Adriana, Lomas David A, Vallier Ludovic, Murphy George J, Mostoslavsky Gustavo, Spira Avrum, Shirihai Orian S, Ramirez Maria I, Gadue Paul, Kotton Darrell N: Emergence of a stage-dependent human liver disease signature with directed differentiation of alpha-1 antitrypsin-deficient iPS cells. Stem cell reports 4(5): 873-85, May 2015.

Antony-Debré Iléana, Manchev Vladimir T, Balayn Nathalie, Bluteau Dominique, Tomowiak Cécile, Legrand Céline, Langlois Thierry, Bawa Olivia, Tosca Lucie, Tachdjian Gérard, Leheup Bruno, Debili Najet, Plo Isabelle, Mills Jason A, French Deborah L, Weiss Mitchell J, Solary Eric, Favier Remi, Vainchenker William, Raslova Hana: Level of RUNX1 activity is critical for leukemic predisposition but not for thrombocytopenia. Blood 125(6): 930-40, Feb 2015.

Ge Jingping, Apicella Marisa, Mills Jason A, Garçon Loïc, French Deborah L, Weiss Mitchell J, Bessler Monica, Mason Philip J: Dysregulation of the Transforming Growth Factor β Pathway in Induced Pluripotent Stem Cells Generated from Patients with Diamond Blackfan Anemia. PloS one 10(8): e0134878, 2015.

Vasireddy Vidyullatha, Mills Jason A, Gaddameedi Rajashekhar, Basner-Tschakarjan Etiena, Kohnke Monika, Black Aaron D, Alexandrov Krill, Zhou Shangzhen, Maguire Albert M, Chung Daniel C, Mac Helen, Sullivan Lisa, Gadue Paul, Bennicelli Jeannette L, French Deborah L, Bennett Jean: Correction: AAV-Mediated Gene Therapy for Choroideremia: Preclinical Studies in Personalized Models. PloS one 10(6): e0129982, 2015.

Paluru Prasuna, Hudock Kristin M, Cheng Xin, Mills Jason A, Ying Lei, Galvão Aline M, Lu Lin, Tiyaboonchai Amita, Sim Xiuli, Sullivan Spencer K, French Deborah L, Gadue Paul: The negative impact of Wnt signaling on megakaryocyte and primitive erythroid progenitors derived from human embryonic stem cells. Stem cell research 12(2): 441-51, Mar 2014.

Tiyaboonchai Amita, Mac Helen, Shamsedeen Razveen, Mills Jason A, Kishore Siddarth, French Deborah L, Gadue Paul: Utilization of the AAVS1 safe harbor locus for hematopoietic specific transgene expression and gene knockdown in human ES cells. Stem cell research 12(3): 630-637, Feb 2014.

Sullivan Spencer K, Mills Jason A, Koukouritaki Sevasti B, Vo Karen K, Lyde Randolph B, Paluru Prasuna, Zhao Guoha, Zhai Li, Sullivan Lisa M, Wang Yuhuan, Kishore Siddharth, Gharaibeh Eyad Z, Lambert Michele P, Wilcox David A, French Deborah L, Poncz Mortimer, Gadue Paul: High-level transgene expression in induced pluripotent stem cell-derived megakaryocytes: correction of Glanzmann thrombasthenia. Blood 123(5): 753-7, Jan 2014.

Hubbard Jesse J, Sullivan Spencer K, Mills Jason A, Hayes Brian J, Torok-Storb Beverly J, Ramakrishnan Aravind: Efficient iPS cell generation from blood using episomes and HDAC inhibitors. Journal of visualized experiments : JoVE(92), e52009, 2014.

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Last updated: 08/25/2016
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