Diana Elena Stanescu, MD

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Assistant Professor of Pediatrics
Department: Pediatrics
Graduate Group Affiliations

Contact information
34th & Civic Center Blvd.
Philadelphia, PA 19104
Office: 2676482541
Fax: 2155907033
Spiru Haret National College, Bucharest, Romania, 1999.
Carol Davila University of Medicine and Pharmacy, Bucharest, Romania, 2005.
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Description of Research Expertise

I am a physician scientist with an interest in the developmental biology of pancreatic endocrine cells. My current research is aimed at bridging the existing knowledge gap between the maturation of fetal beta cells and stem-cell derived beta cells. The results of this research could lead to improvements in vitro differentiation protocols and help in providing a cell replacement therapy for patients with type 1 diabetes.

Main lab projects:
1) Human pancreatic endocrine cell maturation - we use functional assays and single cell RNA seq to characterize the maturation of human beta cells in development.
2) Role of Slc38a5 in alpha cell specification and maturation in mouse development - we use mouse models to characterize the role of the glycine and glutamine transporter - Slc38a5 - in alpha cell development.

Rotation projects:
1) Single cell RNA-seq data analysis of human fetal endocrine cells
2) Characterization of alpha and beta cell maturational markers

Lab personel:
Juxiang Yang, PhD
Jessica Yang, BS
Venkata Jonnakuti, BS

Selected Publications

Good AL Cannon CE, Haemmerle MW, Yang J, Stanescu DE, Doliba NM, Birnbaum MJ1 Stoffers DA.: JUND regulates pancreatic β cell survival during metabolic stress. Mol Metab. 2019 Apr 11. pii: S2212-8778(18)31228-6. 2019.

Cardenas-Diaz FL, Osorio-Quintero C, Diaz-Miranda MA, Kishore S, Leavens K, Jobaliya C, Stanescu D, Ortiz-Gonzalez X, Yoon C, Chen CS, Haliyur R, Brissova M, Powers AC, French DL, Gadue P: Modeling Monogenic Diabetes using Human ESCs Reveals Developmental and Metabolic Deficiencies Caused by Mutations in HNF1A. Cell Stem Cell. 2019 Aug 1;25(2):273-289.e5. 2019.

Kim J, Stanescu DE, Won KJ: CellBIC: bimodality-based top-down clustering of single-cell RNA sequencing data reveals hierarchical structure of the cell type. Nucleic Acids Res. 2018 Nov 30;46(21):e124 2018.

Stanescu DE, Yu R, Won KJ, Stoffers DA: Single cell transcriptomic profiling of mouse pancreatic progenitors. Physiological Genomics 49(2): 589-604, October 2016.

Tiyaboonchai A, Cardenas-Diaz FL, Ying L, Maguire JA, Sim X, Gagne AL, Stanescu DE, Hughes N, De Leon D, French DL, Gadue P: GATA6 Plays an Important Role in the Induction of Human Definitive Endoderm, Development of the Pancreas, and Functionality of Pancreatic β Cells. Stem Cell Reports 8(3): 589-604, October 2016.

Henley KD*, Stanescu DE*, Kropp PA*, Wright CVE, Won KJ, Stoffers DA, Gannon M: Threshold-dependent cooperative function of Pdx1 and Oc1 within pancreatic progenitors establishes competency for later β-cell maturation. Cell Reports June 2016 Notes: 5(12):2637-50 *equal first authors.

Stanescu Diana E, Hughes Nkecha, Patel Puja, De León Diva D: A novel mutation in GATA6 causes pancreatic agenesis. Pediatric diabetes 16(1): 67-70, February 2015.

Weber DR, Stanescu DE, Semple R, Holland C, Magge SN: Continuous subcutaneous IGF-1 therapy via insulin pump in a patient with Donohue syndrome. J Pediatr Endocrinol Metab. 27(11-12): 1237-41, August 2014.

Stanescu Diana E, Hughes Nkecha, Kaplan Bernard, Stanley Charles A, De León Diva D: Novel presentations of congenital hyperinsulinism due to mutations in the MODY genes: HNF1A and HNF4A. The Journal of clinical endocrinology and metabolism 97(10): E2026-30, Oct 2012.

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Last updated: 06/01/2020
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