Philip R. Johnson

faculty photo
Professor of Pediatrics
Director, Joseph Stokes Research Institute, The Children's Hospital of Philadelphia
Chief Scientific Officer & Executive Vice President, The Children's Hospital of Philadelphia
Department: Pediatrics

Contact information
The Children's Hospital of Philadelphia
Room 1216B, Abramson Research Center
3615 Civic Center Blvd
Philiadelphia, PA 19104
Office: 267-426-0351
Fax: 267-426-0363
Graduate Group Affiliations
B.A. (Chemistry and Interdisciplinary Studies )
University of North Carolina - Chapel Hill, 1976.
M.D. (Medicine)
University of North Carolina - Chapel Hill, 1980.
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Description of Research Expertise

Research Interests
Vaccine development; viral vectors; gene transfer; HIV and SIV

Key words: SIV; HIV; AAV; Vaccine; Gene Transfer

Description of Research
Our laboratory has two main programs: (i) vaccine development (especially for HIV) and (ii) development of recombinant adeno-associated virus (rAAV) as a vector for human gene transfer. We have 25 years of experience in developing viral vectors for the delivery of vaccine antigens and the use of animal models to evaluate vaccine strategies. Our main interest continues to be prophylactic vaccines for HIV. Candidate vaccines developed in our laboratory have reached phase II human testing. More recently, we have begun the explore the use of rAAV vectors to deliver genes that encode antibodies which neutralize HIV (Nat Med. 2009; 15:901-6) A human phase I trial of this strategy is being planned. The laboratory also continues to seek novel uses for rAAV vectors in the treatment of human and animal diseases.

Rotation Projects
A variety of projects are available.

Lab personnel:
Jeff Connell - Quality & Safety Manager, Mary Connell - Research Lab Director, Erica Koff - Research Technician I, Bruce Schnepp - Scientist, Teihui Liu - Research Associate II.

Selected Publications

Oscherwitz J, Yu F, Jacobs JL,Liu T-H, Johnson PR, Cease IB: A synthetic peptide vaccine targeting a cryptic neutralizing epitope in domain 2 of Bacillus anthracis protective antigen. Infection and Immunity (published online ahead of print) June 2009.

Schnepp Bruce C, Jensen Ryan L, Clark K Reed, Johnson Philip R: Infectious molecular clones of adeno-associated virus isolated directly from human tissues. Journal of virology 83(3): 1456-64, Feb 2009.

Liu Te-Hui, Oscherwitz Jon, Schnepp Bruce, Jacobs Jana, Yu Fen, Cease Kemp B, Johnson Philip R: Genetic vaccines for anthrax based on recombinant adeno-associated virus vectors. Molecular Therapy 17(2): 373-9, Feb 2009.

Johnson PR, Schnepp BC, Zhang J, Connell MJ, Greene SM, Yuste E, Desrosiers RC, and Clark KR.: Vector-mediated gene transfer engenders long-lived neutralizing activity and protection against SIV infection in monkeys. Nature Medicine in press, 2009.

Clément N, Battaglioli G, Jensen RL, Schnepp BC, Johnson PR, St. George K, and Linden RM. : Prevalence of Human Bocavirus in Human Tonsils and Adenoids. Emer Infect Dis in press, 2009.

Mehendale S, van Lunzen J, Clumeck N, Rockstroh J, Vets E, Johnson PR, Anklesaria P, Barin B, Boaz M, Kochhar S, Lehrman J, Schmidt C, Peeters M, Schwarze-Zander C, Kabamba K, Glaunsinger T, Sahay S, Thakar M, Paranjape M, Gilmour J, Excler J-L, Fast P, and Heald AE. : A Phase 1 Study to Evaluate the Safety and Immunogenicity of a Recombinant HIV-1 Subtype C Adeno-Associated Virus Vaccine. AIDS Res Hum Retroviruses 24: 873-80, 2008.

Harrington Patrick R, Connell Mary J, Meeker Rick B, Johnson Philip R, Swanstrom Ronald: Dynamics of simian immunodeficiency virus populations in blood and cerebrospinal fluid over the full course of infection. The Journal of infectious diseases 196(7): 1058-67, Oct 2007.

Schnepp Bruce C, Jensen Ryan L, Chen Chun-Liang, Johnson Philip R, Clark K Reed: Characterization of adeno-associated virus genomes isolated from human tissues. Journal of virology 79(23): 14793-803, Dec 2005.

Chen Chun-Liang, Jensen Ryan L, Schnepp Bruce C, Connell Mary J, Shell Richard, Sferra Thomas J, Bartlett Jeffrey S, Clark K Reed, Johnson Philip R: Molecular characterization of adeno-associated viruses infecting children. Journal of virology 79(23): 14781-92, Dec 2005.

Johnston Robert E, Johnson Philip R, Connell Mary J, Montefiori David C, West Ande, Collier Martha L, Cecil Chad, Swanstrom Ronald, Frelinger Jeffrey A, Davis Nancy L: Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660. Vaccine 23(42): 4969-79, Oct 2005.

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Last updated: 03/18/2013
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