Perelman School of Medicine at the University of Pennsylvania

Biomedical Postdoctoral Council

Header Image

Spatial and temporal regulation of Collagen 2 labeling in the developing intervertebral disk by Dr. Tower

Research Symposium

The annual BPC Postdoctoral Symposium will be held on November 5th, 2018, in the Smilow Center for Translational Research. A detailed schedule will be posted closer to the date. Registration is still open, but we are no longer accepting abstracts. Our keynote speaker is Dr. Richardy Dorsky. Please click on the appropriate link for more details.

 

About the Photo 

This image is part of a collaboration between Robert J Tower, Yulong Wei and Ling Qin in the department of Orthopaedic Surgery at UPenn and Yejia Zhang and Tian Zouzhen at the Translational Musculoskeletal Research Center (TRMC) at the Philadelphia VA Medical Center (VAMC). This image is of the mouse tail vertebral disc shortly after birth from a transgenic mouse containing the collagen type II promoter driven Cre recombinase (Col2-Cre) and a floxed tdTomato, labeling all cells either expressing or derived from a Col2-expressing cell. Wholemount sections were also stained with DAPI to label nuclei and with Phalloidin to label F-actin in cells. This uniform labeling of both the nucleus polposus (inner disk) and the annulus fibrosis (outer rings surrounding NP) is in contrast to mice in which the Col2 labeling is driven by an inducible Cre (CreERt) which shows labeling of only the inner AF. This image helped demonstrate a temporal and spatial regulation of collagen proteins during disc development.