Cryo-EM Screening and Training Facility

The Cryo-EM Screening and Training Facility is the part of the Electron Microscopy Resource Laboratory that provides training and access to state-of the art cryo electron microscopy (cryo-EM) and cryo electron tomography (cryo-ET) for structural investigation of macromolecules and cells. The facility houses two FEI microscopes (T12 and TF20), sample preparation instruments such as Vitrobot as well as two Leica cryo plungers, and all the accessories needed to perform sample screening using negative stain imaging and cryo-EM. The facility is available to Penn research groups and external academic research groups in the greater Philadelphia area.

The goal of The Cryo-EM Screening and Training Facility is to train users to self-sufficiency and proficiency in microscope operation and sample preparation with the ultimate goal of creating independent users who require minimal assistance from staff.

We offer the following services at the Cryo-EM Screening and Training Facility:

  • Training on the two FEI microscopes (T12 and TF20) for negative stain imaging and cryo-EM.
  • Training on sample preparation instruments such as Vitrobot and two Leica cryo plungers.
  • Pay-per-service for negative stain imaging. Note that this service is only available for Penn and CHOP users.

Note: The Cryo-EM Screening and Training Facility does not provide cryo-EM data processing and reconstruction services.

To request services please contact us at: SCREEN_SERV@lists.upenn.edu


Cryo-EM Screening and Training Facility Director
Sudheer Molugu, Ph.D.