Molecular Profiling Facility
Core Description: Now part of the the Penn Genomics Analysis Core (previously known as the Molecular Profiling Facility) provides an integrated set of services for DNA and RNA profiling. These services are delivered by experienced genomics professionals, including a focused bioinformatics support staff. PSOM faculty benefit from consultations and training available throughout their projects, including during experimental design and budget development, sample accrual, Facility quality control assays and lab work, data management and analyses, and manuscript preparation. The core supports quantitative RNA profiling by Affymetrix GeneChips, Illumina BeadChips, real-time PCR, Sequenom custom multiplex assays, Fluidigm, Luminex and deep sequencing. DNA profiling of custom panels of sequence polymorphisms are conducted by quantitative PCR, Sequenom assays, and Illumina GoldenGate genotyping, while whole-genome assays are available on Affymetrix SNP GeneChip and Illumina Infinium platforms. Whole-exome and targeted genomic regions can be resequenced on an Illumina Genome Analyzer deep sequencer. Several other services including microRNA profiling, epigenetic DNA assays, and translational molecular diagnostics for clinical research are offered using these platforms.