Histology and Gene Expression Co-Op

 

The Histology and Gene Expression Co-Op provides expert professional services for primary laboratories within the CVI with contribution payment (for staff salary). The Histology and Gene Expression Co-Op offers all histology-related services including tissue processing, embedding, sectioning, staining, immunocytochemistry, and in situ hybridization.

Training:

• Tissue training
• IHC Training
• Antibody Training

Mark L. Kahn, M.D., Faculty Advisor

Smilow TRC Room 11-123
Phone: 215-898-9007
Email: markkahn@pennmedicine.upenn.edu

Rajan Jain, M.D., Faculty Advisor

Smilow TRC Room 9-102
Phone: 215-573-3011
Email: jainr@pennmedicine.upenn.edu

Additional Contact Information:

Jisheng Yang

Phone: 215-573-2328
Location: Smilow TRC 11-183
Email: jisheng@pennmedicine.upenn.edu

Li Li

Phone: 215-573-2327
Location: Smilow TRC 9-188
Email: li10@pennmedicine.upenn.edu

Su Zhou

Phone: 215-573-2781
Location: Smilow TRC 11-178
Emil: zhou5@pennmedicine.upenn.edu

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Instructions and Forms:

Instructions

• Submit Samples for Paraffin Section
• Submit Samples for Frozen Section
• Submit Embedded Blocks for Sectioning
• Submit slides for HE or Eosin staining
• Submit slides for other staining
• Submit Paraffin slides for Immunohistochemistry (IHC) staining
• Submit Frozen slides for Immunohistochemistry (IHC) staining
• Submit IHC form
• Submit sections for RNA Scope In Situ Hybridization

Forms

• Tissue Form
• IHC Form [FileMaker Pro 12]
• In Situ Form RNA Scope [FileMaker Pro 12]

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Service Instructions and Forms:

Instructions

• Request a service
• Submit adult samples for paraffin section
• Submit mouse embryos for paraffin section
• Submit slides for HE or Eosin staining
• Submit slides for other staining
• Submit paraffin slides for Immunohistochemistry (IHC) staining

Forms

• Service Request Form
• Tissue Form
• Service Staining Form

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References and Protocols:

References

• Number of sets and number of slide
• Sample tissue from form 1
• Sample tissue from form 2
• Test new Ab for IHC
• Antibody list
• IHC template list .fm12
• IHC template list .htm
• IHC double staining list

Histology Protocols

• How to Make 4% PFA
• How to fix and dehydrate tissue
• Sample preparation for E17.5-P0
• How to Perform H&E Staining
• Decalcification Using EDTA
• McMannus' Periodic Acid Schiff's (PAS) Stain
• Verhoeff-Van Gieson (Elastin) Stain
• Masson's Trichrome Stain
• Modified Movat's Pentachrome Stain

General IHC Protocols

• Prepare Tissue for IHC on Paraffin Sections
• Prepare Tissue for IHC in Frozen Sections
• Prepare Tissue for Whole Mount IHC
• Prepare Cultured Cells for IHC
• Protein A Purification of Polyclonal Ab
• Antigen Retrieval
• Alexa Fluo 488 or 568 on paraffin sections
• ImmPress-TSA on paraffin sections
• Double ImmPress-TSA on paraffin sections
• ImmP-HRP on paraffin sections
• Alexa Fluo 488 or 568 on frozen sections

In Situ Hybridization Protocols

• How to Process Embryos for Whole Mount In Situs
• Whole Mount In Situs Protocol
• Tissue Process for RNSscope Insitu
• RNAscope multiplex Fluorescent user manual

Apoptsis Protocol

• Apoptosis Using Paraffin Embedded Sections

Individual IHC Protocols

• Rat Anti-PECAM on Paraffin Sections ( ImmPTSA)
• Goat Anti-GFP on paraffin Sections (Alexa 488)
• Rabbit Anti-RFP on paraffin Sections (Alexa 568)
• Rabbit Anti-P-ERK on paraffin Sections (ImmPHRP)
• Mouse Anti-Brdu on paraffin Sections (Alexa 568)

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