Perelman School of Medicine at the University of Pennsylvania

Grice Lab



Singh TP, Carvalho AM, Sacramento LA, Grice EA, Scott P.  Microbiota instruct IL-17A-producing innate lymphoid cells to promote skin inflammation in cutaneous leishmaniasis.  PLoS Pathog. 2021 Oct 26;17(10):e1009693.doi: 10.1371/journal.ppat.1009693.

Zeeuwen PLJM, Grice EA. Skin microbiome and antimicrobial peptides. Editorial - Exp Dermatol. 2021 Oct;30(10):1362-1365.doi: 10.1111/exd.14454.

Bhuju J, Olesen KM, Muenyi CS, Patel TS, Read RW, Thompson L, Skalli O, Zheng Q, Grice EA, Sutter CH, Sutter TR. Cutaneous Effects of In Utero and Lactational Exposure of C57BL/6J Mice to 2,3,7,8-Tetrachlorodibenzo- p-dioxin.  Toxics. 2021 Aug 20;9(8):192.doi: 10.3390/toxics9080192.

Uberoi A, Bartow-McKenney C, Zheng Q, Flowers L, Campbell A, Knight SAB, Chan N, Wei M, Lovins V, Bugayev J, Horwinski J, Bradley C, Meyer J, Crumrine D, Sutter CH, Elias P, Mauldin E, Sutter TR, Grice EA.  Commensal microbiota regulates skin barrier function and repair via signaling through the aryl hydrocarbon receptor.  Cell Host Microb.  2021 Aug 11;29(8):1235-1248.e8. doi: 10.1016/j.chom.2021.05.011.

Dalton KR, Ruble K, Redding LE, Morris DO, Mueller NT, Thorpe RJ Jr, Agnew J, Carroll KC, Planet PJ, Rubenstein RC, Chen AR, Grice EA, Davis MF.  Microbial Sharing between Pediatric Patients and Therapy Dogs during Hospital Animal-Assisted Intervention Programs.  Microorganisms. 2021 May 13;9(5):1054. doi: 10.3390/microorganisms9051054.

Byeon J, Blizinsky KD, Persaud A, Findley K, Lee JJ, Buscetta AJ, You S, Bittinger K, Minniti CP, Bonham VL, Grice EA.  Insights into the skin microbiome of sickle cell disease leg ulcers.  Wound Repair Regen. 2021 May 8. doi: 10.1111/wrr.12924.

Wang G, Sweren E, Liu H, Wier E, Alphonse MP, Chen R, Islam N, Li A, Xue Y, Chen J, Park S, Chen Y, Lee S, Wang Y, Wang S, Archer NK, Andrews W, Kane MA, Dare E, Reddy SK, Hu Z, Grice EA, Miller LS, Garza LA.  Bacteria induce skin regeneration via IL-1β signaling.  Cell Host Microbe. 2021 May 12;29(5):777-791.e6. doi: 10.1016/j.chom.2021.03.003.

Zheng Q, Capell BC, Parekh V, O'Day C, Atillasoy C, Bashir HM, Yeh C, Shim EH, Prouty SM, Dentchev T, Lee V, Wushanley L, Kweon Y, Suzuki-Horiuchi Y, Pear W, Grice EA, Seykora JT.  Whole-Exome and Transcriptome Analysis of UV-Exposed Epidermis and Carcinoma In Situ Reveals Early Drivers of Carcinogenesis.  J Invest Dermatol. 2021 Feb;141(2):295-307.e13. doi: 10.1016/j.jid.2020.05.116.


Flowers L, Grice EA.  The Skin Microbiota: Balancing Risk and Reward.  Cell Host Microbe. 2020 Aug 12;28(2):190-200. doi: 10.1016/j.chom.2020.06.017.

Bradley CW, Lee FF, Rankin SC, Kalan LR, Horwinski J, Morris DO, Grice EA, Cain CL.  The otic microbiota and mycobiota in a referral population of dogs in eastern USA with otitis externa.  Vet Dermatol.  2020 Jun;31(3):225-e49. doi:10.1111/vde.12826.


Knight R, Ley RE, Raes J, Grice EA.  Expanding the scope and scale of microbiome research.  Genome Biol.  2019 Sep 5;20(1):191. doi: 10.1186/s13059-019-1804-2.

Deusenbery CB, Kalan L, Meisel JS, Gardner SE, Grice EA, Spiller KL.  Human macrophage response to microbial supernatants from diabetic foot ulcers.  Wound Repair Regen. 2019 Nov;27(6):598-608. doi: 10.1111/wrr.12752.

Kalan LR, Meisel JS, Loesche MA, Horwinski J, Soaita I, Chen X, Uberoi A, Gardner SE, Grice EA. Strain- and Species-Level Variation in the Microbiome of Diabetic Wounds Is Associated with Clinical Outcomes and Therapeutic Efficacy.  Cell Host Microbe. 2019 May 8;25(5):641-655.e5.doi: 10.1016/j.chom.2019.03.006.

Grogan MD, Bartow-McKenney C, Flowers L, Knight SAB, Uberoi A, Grice EA.  Research Techniques Made Simple: Profiling the Skin Microbiota.  J Invest Dermatol. 2019 Apr;139(4):747-752.e1. doi: 10.1016/j.jid.2019.01.024.


Kalan L, Grice EA. Fungi in the Wound Microbiome. Adv Wound Care (New Rochelle). 2018 Jul 1;7(7):247-255. doi: 10.1089/wound.2017.0756. Review.PMID: 29984114

Zheng Q, Bartow-McKenney C, Meisel JS, Grice EA. HmmUFOtu: An HMM and phylogenetic placement based ultra-fast taxonomic assignment and OTU picking tool for microbiome amplicon sequencing studies. Genome Biol. 2018 Jun 27;19(1):82. doi: 10.1186/s13059-018-1450-0. PMID: 29950165

Bartow-McKenney C, Hannigan GD, Horwinski J, Hesketh P, Horan AD, Mehta S, Grice EA. The microbiota of traumatic, open fracture wounds is associated with mechanism of injury. Wound Repair Regen. 2018 May 26. doi: 10.1111/wrr.12642. [Epub ahead of print] PMID: 29802752

SanMiguel AJ, Meisel JS, Horwinski J, Zheng Q, Bradley CW, Grice EA. Antiseptic Agents Elicit Short-Term, Personalized, and Body Site-Specific Shifts in Resident Skin Bacterial Communities. J Invest Dermatol. 2018 May 9. pii: S0022-202X(18)31959-6. doi: 10.1016/j.jid.2018.04.022. [Epub ahead of print] PMID: 29753031

Fiala CA, Abbott LI, Carter CD, Hillis SL, Wolf JS, Schuster M, Dulski R, Grice EA, Rakel BA, Gardner SE. Severe pain during wound care procedures: A cross-sectional study protocol. J Adv Nurs. 2018 May 7. doi: 10.1111/jan.13699. [Epub ahead of print] PMID: 9733454

Loesche MA, Farahi K, Capone K, Fakharzadeh S, Blauvelt A, Duffin KC, DePrimo SE, Muñoz-Elías EJ, Brodmerkel C, Dasgupta B, Chevrier M, Smith K, Horwinski J, Tyldsley A, Grice EA. Longitudinal Study of the Psoriasis-Associated Skin Microbiome during Therapy with Ustekinumab in a Randomized Phase 3b Clinical Trial. J Invest Dermatol. 2018 Mar 17. pii: S0022-202X(18)31731-7. doi: 10.1016/j.jid.2018.03.1501. [Epub ahead of print] PMID: 9559344

Meisel JS, Sfyroera G, Bartow-McKenney C, Gimblet C, Bugayev J, Horwinski J, Kim B, Brestoff JR, Tyldsley AS, Zheng Q, Hodkinson BP, Artis D, Grice EA. Commensal microbiota modulate gene expression in the skin. Microbiome. 2018 Jan 30;6(1):20. doi: 10.1186/s40168-018-0404-9. PMID: 29378633


Grice EA, Dawson TL Jr. Host-microbe interactions: Malassezia and human skin. Curr Opin Microbiol. 2017 Dec;40:81-87. doi: 10.1016/j.mib.2017.10.024. Epub 2017 Nov 12. Review. PubMed PMID: 29141240.

Coughlin CC, Swink SM, Horwinski J, Sfyroera G, Bugayev J, Grice EA, Yan AC. The preadolescent acne microbiome: A prospective, randomized, pilot study investigating characterization and effects of acne therapy. Pediatr Dermatol. 2017 Nov;34(6):661-664. doi: 10.1111/pde.13261. Epub 2017 Oct 11. PubMed PMID: 29024079.

Gimblet C, Meisel JS, Loesche MA, Cole SD, Horwinski J, Novais FO, Misic AM,Bradley CW, Beiting DP, Rankin SC, Carvalho LP, Carvalho EM, Scott P, Grice EA.Cutaneous Leishmaniasis Induces a Transmissible Dysbiotic Skin Microbiota thatPromotes Skin Inflammation. Cell Host Microbe. 2017 Jul 12;22(1):13-24.e4. doi:10.1016/j.chom.2017.06.006. Epub 2017 Jun 29. PubMed PMID: 28669672.

SanMiguel AJ, Meisel JS, Horwinski J, Zheng Q, Grice EA. Topical antimicrobialtreatments can elicit shifts to resident skin bacterial communities and reducecolonization by Staphylococcus aureus competitors. Antimicrob Agents Chemother.2017 Jun 19. pii: AAC.00774-17. doi: 10.1128/AAC.00774-17.PubMed PMID: 28630195.

Plichta JK, Gao X, Lin H, Dong Q, Toh E, Nelson DE, Gamelli RL, Grice EA,Radek KA. Cutaneous Burn Injury Promotes Shifts in the Bacterial Microbiome inAutologous Donor Skin: Implications for Skin Grafting Outcomes. Shock. 2017 Mar31. doi: 10.1097/SHK.0000000000000874. PubMed PMID:28368977.

Hannigan GD, Zheng Q, Meisel JS, Minot SS, Bushman FD, Grice EA. Evolutionary and functional implications of hypervariable loci within the skin virome. PeerJ. 2017 Feb 7;5:e2959. doi: 10.7717/peerj.2959. (PMID: 28194314)

Jacquelyn S. Meisel and Elizabeth A. Grice, Chapter 4 - The Human Microbiome, In Genomic and Precision Medicine (Third Edition), edited by Geoffrey S. Ginsburg and Huntington F. Willard, Academic Press, Boston, 2017, Pages 63-77, ISBN 9780128006818,


Kalan L, Gardner SE, Grice EA. Reply to "Understanding the Role of Fungi inChronic Wounds". MBio. 2016 Dec 6;7(6). pii: e02033-16. doi:10.1128/mBio.02033-16. (PMID: 27923927)

Findley K, Williams DR, Grice EA, Bonham VL. Health Disparities and theMicrobiome. Trends Microbiol. 2016 Nov;24(11):847-850. doi:10.1016/j.tim.2016.08.001. (PMID: 27712950)

Loesche M, Gardner SE, Kalan L, Horwinski J, Zheng Q, Hodkinson BP, Tyldsley AS, Franciscus CL, Hillis SL, Mehta S, Margolis DJ, Grice EA. Temporal Stability in Chronic Wound Microbiota Is Associated With Poor Healing. J Invest Dermatol. 2017 Jan;137(1):237-244. doi: 10.1016/j.jid.2016.08.009. (PMID: 27566400)

Zheng Q, Grice EA. AlignerBoost: A Generalized Software Toolkit for Boosting Next-Gen Sequencing Mapping Accuracy Using a Bayesian-Based Mapping Quality Framework. PLoS Comput Biol. 2016 Oct 5;12(10):e1005096. doi:10.1371/journal.pcbi.1005096. (PMID: 27706155)

Kalan L, Loesche M, Hodkinson BP, Heilmann K, Ruthel G, Gardner SE, Grice EA. Redefining the Chronic-Wound Microbiome: Fungal Communities Are Prevalent, Dynamic, and Associated with Delayed Healing. MBio. 2016 Sep 6;7(5). pii: e01058-16. doi: 10.1128/mBio.01058-16. (PMID: 27601572)

Ibiza S, García-Cassani B, Ribeiro H, Carvalho T, Almeida L, Marques R, Misic AM, Bartow-McKenney C, Larson DM, Pavan WJ, Eberl G, Grice EA, Veiga-Fernandes H. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence. Nature. 2016 Jul 21;535(7612):440-3. (PMID: 27409807)

Bradley CW, Morris DO, Rankin SC, Cain CL, Misic AM, Houser T, Mauldin EA, Grice EA. Longitudinal Evaluation of the Skin Microbiome and Association with Microenvironment and Treatment in Canine Atopic Dermatitis. J Invest Dermatol. 2016 Feb 6. pii: S0022-202X(16)00455-3. doi: 10.1016/j.jid.2016.01.023. (PMID: 26854488)

Meisel JS, Hannigan GD, Tyldsley AS, SanMiguel AJ, Hodkinson BP, Zheng Q, Grice EA. Skin Microbiome Surveys Are Strongly Influenced by Experimental Design.
J Invest Dermatol. 2016 May;136(5):947-56. doi: 10.1016/j.jid.2016.01.016. (PMID: 26829039)


Hannigan GD, Meisel JS, Tyldsley AS, Zheng Q, Hodkinson BP, SanMiguel AJ, Minot S, Bushman FD, Grice EA. The Human Skin Double-Stranded DNA Virome: Topographical and Temporal Diversity, Genetic Enrichment, and Dynamic Associations with the Host Microbiome. MBio. 2015 Oct 20;6(5). pii: e01578-15. doi: 10.1128/mBio.01578-15. (PMID: 26489866)

Grice EA. The intersection of microbiome and host at the skin interface: genomic- and metagenomic-based insights. Genome Res. 2015 Oct;25(10):1514-20. doi: 10.1101/gr.191320.115. (PMID: 26430162)

Gimblet C, Loesche MA, Carvalho L, Carvalho EM, Grice EA, Artis D, Scott P. IL-22 Protects against Tissue Damage during Cutaneous Leishmaniasis. PLoS One. 2015 Aug 18;10(8):e0134698. doi: 10.1371/journal.pone.0134698. eCollection 2015. (PMID: 26285207)

Gould L, Abadir P, Brem H, Carter M, Conner-Kerr T, Davidson J, DiPietro L, Falanga V, Fife C, Gardner S, Grice E, Harmon J, Hazzard WR, High KP, Houghton P, Jacobson N, Kirsner RS, Kovacs EJ, Margolis D, McFarland Horne F, Reed MJ, Sullivan DH, Thom S, Tomic-Canic M, Walston J, Whitney JA, Williams J, Zieman S, Schmader K. (2015) Chronic wound repair and healing in older adults: Current status and future researchJournal of the American Geriatric Society. Epub ahead of print. (PMID:25753048).

Misic AM, Davis MF, Tyldsley AS, Hodkinson BP, Tolomeo P, Hu B, Nachamkin I, Lautenbach E, Morris DO, Grice EA. (2015) The shared microbiota of humans and companion animals as evaluated from Staphylococcus carriage sites.Microbiome. 3:2. (PMID:25705378).

Hannigan GD, Pulos N, Grice EA, and Mehta S. (2015) Current concepts and ongoing research in the prevention and treatment of open fracture infections. Advances in Wound Care. 4(1):59-74. (PMID:25566415).

Hodkinson BP and Grice EA. (2015) Next-generation sequencing: A review of technologies and tools for wound microbiome research. Advances in Wound Care. 4(1):50-58. (PMID:25566414).

SanMiguel A and Grice EA. (2015) Interactions between host factors and the skin microbiome.Cellular and Molecular Life Sciences. 72(8):1499-515. (PMID:25548803).

Rafail S, Kourtzelis I, Foukas PG Markiewski MM, DeAngelis RA, Guariento M, Ricklin D, Grice EA, Lambris JD. (2015) Complement deficiency promotes cutaneous wound healing in mice.  Journal of Immunology. 194(3):1285-91. (PMID:25548229).

Prokop-Prigge KA, Mansfield CJ, Parker MR, Thaler E, Grice EA, Wysocki CJ, Preti G. (2015)  Ethnic/racial and genetic influences on cerumen odorant profile.Journal of Chemical Ecology. 41(1):67-74. (PMID:25501636).

Gould L, Abadir P, Brem H, Carter M, Conner-Kerr T, Davidson J, DiPietro L, Falanga V, Fife C, Gardner S, Grice E, Harmon J, Hazzard WR, High KP, Houghton P, Jacobson N, Kirsner RS, Kovacs EJ, Margolis D, McFarland Horne F, Reed MJ, Sullivan DH, Thom S, Tomic-Canic M, Walston J, Whitney J, Williams J, Zieman S, Schmader K. (2014) Chronic wound repair and healing in older adults: Current status and future research. Wound Repair and Regeneration. Epub ahead of print. (PMID:25486905).

Findley K and Grice EA. (2014) Skin microbiome: a focus on pathogens and their association with skin disease. PLOS Pathogens. 10(10):e1004436. (PMID:25393405).

Grice EA. (2014) The skin microbiome: potential for novel diagnostic and therapeutic approaches to cutaneous disease. Seminars in Cutaneous Medicine and Surgery. 33(2):98-103. (PMID:25085669).

Misic AM, Gardner SE, and Grice EA. (2014) The wound microbiome: Modern approaches to examining the role of microorganisms in impaired chronic wound healing. Advances in Wound Care. 1;3(7):502-510. (PMID:25032070).

Hannigan GD, Hodkinson BP, McGinnis K, Tyldsley AS, Anari JB, Horan AD, Grice EA*, Mehta S*. (2014) Culture-independent pilot study of microbiota colonizing open fractures and association with severity, mechanism, location, and complication from presentation to early outpatient follow-up. Journal of Orthopedic Research. 32(4):597-605. (PMID:24395335).

Hannigan GD and Grice EA. (2013) Microbial ecology of the skin in the era of metagenomics and molecular microbiology. Cold Spring Harbor Perspectives in Medicine. 3:a015362 (PMID:24296350)

Chehoud C, Rafail S, Tyldsley AS, Seykora JT, Lambris JD, Grice EA. (2013) Complement modulates the cutaneous microbiome and inflammatory milieu. PNAS. 110:15061-6 (PMID: 23980152)

Gardner SE, Hillis SL, Heilmann K, Segre JA, Grice EA. (2013) The neuropathic diabetic foot ulcer microbiome is associated with clinical factors. Diabetes. 62:923-30 (PMID: 23139351).

Grice EA and Segre JA. (2012) The human microbiome: Our second genome. Annual Review in Human Genetics and Genomics.13: 151-170, Sept 2012 (PMID: 22703178).

Kong HH, Oh J, Deming C, Conlan S, Grice EA, Beatson M, Nomicos E, Polley E, Komarow HD, NISC Comparative Sequencing Program, Murray PR, Turner ML, Segre JA. (2012) Temporal shifts in the skin microbiome associated with atopic dermatitis disease flares and treatment. Genome Research. Feb 6 (PMID: 22310478).

Grice EA and Segre JA. (2012) Interaction of microbiome and innate immune response in chronic wounds. Advances in Experimental Medicine and Biology. 946:55-68. (PMID: 21948362)

Grice EA and Segre JA. (2011) The skin microbiome. Nature Reviews Microbiology, 9:244-53. (PMID: 21407241)

Grice EA, Snitkin ES, Yockey LJ, Bermudez DM, NISC Comparative Sequencing Program, Liechty KW, Segre JA. (2010)Longitudinal shift in diabetic wound microbiota correlates with prolonged defense response. Proceedings of the National Academy of Science USA, 107:14799-804. (PMID: 20668241)

Emison ES, Garcia-Barcelo M, Grice EA, International Hirschsprung Disease Consortium, McCallion AS, Chakravarti A. (2010) Differential contributions of rare and common, coding and noncoding RET mutations to multifactorial Hirschsprung disease liability. American Journal of Human Genetics. 87:60-74. (PMID: 20598273)

Scharschmidt TC, List K, Grice EA, Szabo R, NISC Comparative Sequencing Program, Renaud G, Lee CCR, Wolfsberg TG, Bugge TH, and Segre JA. (2009) Matriptase-deficient mice exhibit ichthyotic skin with a selective shift in skin microbiota. Journal of Investigative Dermatology. 129:2435-42. (PMID:19387477)

Grice EA, Kong HH, Conlan S, Deming CB, Young AC, Davis J, NISC Comparative Sequencing Program, Bouffard GG, Blakesley RW, Murray PR, Green ED, Turner ML, Segre JA. (2009) Topographical and temporal diversity of the human skin microbiome. Science. 234:1190-2. (PMID:19478181)

Grice EA, Kong HH, Renaud G, Young AC, NISC Comparative Sequencing Program, Bouffard GG, Blakesley RW, Wolfsberg TG, Turner ML, Segre JA. (2008) A diversity profile of the human skin microbiota. Genome Research.18:1043-50. (PMID:18502944)

Grice EA and McCallion AS. "RET signaling and Systems Biology" In: Signal Transduction: A Systems Biology Approach. A. Pandey, editor. Humana Press. ISBN: 978-1-58829-487-6.

Fisher S*, Grice EA*, Vinton RM, Bessling SL, Urasaki A, Kawakami K, McCallion AS. (2006) Evaluating the biological relevance of putative enhancers in zebrafish using transposon-mediated transgenesis. Nature Protocols. 1:1297-1305. (PMID:17406414)
*co-first authors

Fisher S*, Grice EA*, Vinton RM, Bessling SL, McCallion AS. (2006) Conservation of RET regulatory function from human to zebrafish in the absence of sequence conservation. Science. 312:276-9. (PMID:16556802)
*co-first authors

Grice EA, Rochelle ES, Green ED, Chakravarti A, McCallion AS. (2005) Evaluation of the RET regulatory landscape reveals the biological relevance of a Hirschsprung disease-implicated enhancer. Human Molecular Genetics14:3837-3845. (PMID:16269442)

Kashuk CS, Stone EA, Grice EA, Portnoy ME, Green ED, Sidow A, Chakravarti A, McCallion AS. (2005) Phenotype-genotype correlation in Hirschsprung disease is illuminated by comparative analysis of the RET protein sequence.Proceedings of the National Academy of Science USA. 102:8949-8954. (PMID:15956201)

Emison ES, McCallion AS, Kashuk CS, Bush RT, Grice E, Lin S, Portnoy ME, Cutler DJ, Green ED, and Chakravarti A. (2005) A common sex-dependent mutation in a RET enhancer underlies Hirschsprung disease risk. Nature. 434:857-863. (PMID:15829955)

Wahlin KJ, Lim L, Grice EA, Campochiaro PA, Zack DJ, Adler R. (2004) A method for analysis of gene expression in isolated mouse photoreceptor and Muller cells. Molecular Vision. 10:366-375. (PMID: 15205663)

Charlet BN, Savkur RS, Singh G, Philips AV, Grice EA, Cooper TA. (2002) Loss of the muscle specific chloride channel in type 1 myotonic dystrophy due to misregulated alternative splicing. Molecular Cell-09. 10:45-53. (PMID:12150906)