Standard
Primers |
Sequence |
Length |
Tm
(° C) |
MW |
e
* (OD/mmol) |
| T7 Promoter |
5'-TAA TAC
GAC TCA CTA TAG GG-3' |
20-mer |
51 |
6125 |
205 |
| T3 Promoter |
5'-CAA TTA
ACC CTC ACT AAA GG-3' |
20-mer |
51 |
6054 |
203 |
| M13 Forward (-20) |
5'-GTA AAA CGA CGG CCA GTG-3' |
18-mer |
54 |
5558 |
188 |
| M13 Forward (-41) |
5'-GGT TTT CCC AGT CAC GAC-3' |
18-mer |
54 |
5451 |
163 |
| M13 Reverse (-27) |
5'-GGA AAC AGC TAT GAC CAT G-3' |
19-mer |
53 |
5846 |
200 |
| M13 Reverse (-48) |
5'-AGC GGA TAA CAA TTT CAC AC-3' |
20-mer |
51 |
6094 |
207 |
| SP6 Promoter |
5'-TAC GAT TTA GGT GAC ACT ATA
G-3' |
22-mer |
53 |
6773 |
225 |
| pBluescript SK |
5'-CGC TCT AGA ACT AGT GGA TC-3' |
20-mer |
55 |
6117 |
194 |
| pBluescript KS |
5'-CTC GAG GTC GAC GGT ATC G-3' |
19-mer |
59 |
5854 |
180 |
| Lambda gt11 (forward) |
5'-GGT GGC GAC GAC TCC TGG AGC
CCG-3' |
24-mer |
71 |
7396 |
223 |
| Lambda gt11 (reverse) |
5'-TTG ACA CCA GAC CAA CTG GTA
ATG-3' |
24-mer |
59 |
7346 |
243 |
| Lambda gt10 (forward) |
5'-AGC AAG TTC AGC CTG GTT AAG-3' |
21-mer |
56 |
6470 |
212 |
| Lambda gt10 (reverse) |
5'-CTT ATG AGT ATT TCT TCC AGG
GTA-3' |
24-mer |
55 |
7349 |
227 |
| pBR322 Bam HI, CW |
5'-CAC TAT CGA CTA CGC GAT CA-3' |
20-mer |
55 |
6046 |
192 |
| pBR322 Bam HI, CCW |
5'-ATG CGT CCG GCG TAG A-3' |
16-mer |
52 |
4922 |
155 |
| pBR322 Eco RI, CW |
5'-GTA TCA CGA GGC CCT T-3' |
16-mer |
50 |
4857 |
148 |
| pBR322 Eco RI, CCW |
5'-GAT AAG CTG TCA AAC-3' |
15-mer |
42 |
4584 |
158 |
| pBR322 HIND III, CW |
5'-GAC AGC TTA TCA TCG-3' |
15-mer |
44 |
4552 |
145 |
| pBR322 HIND III, CCW |
5'-GCA ATT TAA CTG TGA
T-3' |
16-mer |
42 |
4895 |
162 |
| pBR322 Pst I, CW |
5'-GCT AGA GTA AGT AGT T-3' |
16-mer |
44 |
4960 |
168 |
| pBR322 Pst I, CCW |
5'-AAC GAC GAG CGT GAC-3' |
15-mer |
48 |
4611 |
156 |
| pBR322 Sal I, CW |
5'-ATG CAG GAG TCG CAT-3' |
15-mer |
46 |
4617 |
152 |
| pBR322 Sal I, CCW |
5'-AGT CAT GCC CCG CGC-3' |
15-mer |
52 |
4514 |
132 |
| Random Hexamer |
5'-NNNNNN-3' |
6-mer |
58 |
1792 |
12 |
| PolydT20 |
5'-TTT TTT TTT TTT TTT TTT TT-3' |
20-mer |
35 |
6022 |
158 |
