PennPORT

• Current Fellows
• Shanna Bowman, PhD.

Shanna Bowman, PhD.

Department of Pathology,
University of Pennsylvania

Education

• Research mentor: Michael Marks, PhD; Children's Hospital of Philadelphia

Research Topic: Diseases of organelle biogenesis and protein transport

Mammalian cells are organized into specialized organelles, membrane compartments that perform specific functions in the cell. Improper formation of these organelles can result in defects in protein transport and diseases. I am investigating the role of membrane trafficking proteins mutated in the genetic disease, Hermansky-Pudlak Syndrome, in melanosome biogenesis in pigment cells. Melanosomes are a special type of tissue specific organelle, present only in cells that produce melanin. Understanding the mechanisms of melanosome biogenesis serves as an important model to increase our understanding of other tissue specific organelles, disrupted in Hermansky-Pudlak Syndrome, that are crucial for function in processes such as blood clotting and immune system responses to infections.

Teaching Interests:
I am interested in teaching molecular and cellular biology courses to both biology students and non-majors. I would like to develop an introductory biology course for non-majors that uses a set of special topics on diseases, biotechnology, and current issues of interest in biology and medicine to teach molecular and cellular biology.

Courses Taught:
Systems Neuroscience, Spring 2016, Carnegie Mellon University

 
Publications

Peer-Reviewed Publications

• Bowman, S.L., Shiwarski, D.J., Puthenveedu, M.A. Distinct G protein-coupled receptor recycling pathways allow spatial control of downstream G protein signaling. J Cell Biol. 214: 797-806. PMID 27646272.
   
• Tian, X., Irannejad, R., Bowman, S.L., Du, Y., Puthenveedu, M.A., von Zastrow, M., Benovic, J.L. The α-Arrestin ARRDC3 regulates the endosomal residence time and intracellular signaling of the β2-adrenergic receptor. J. Biol. Chem. 291: 14510-25. PMID 27226565.
   
• McGarvey, J.C., Xiao, K.H., Bowman, S.L., Mamonova , T., Zhang , Q., Bisello, A., Sneddon, W.B., Ardura, J.A., Jean-Alphonse, F., Vilardaga, J.P., Puthenveedu, M.A., Friedman, P.A. Actin-sorting nexin 27-retromer complex mediates rapid parathyroid hormone receptor recycling. J. Biol. Chem. 2016. 291: 10986-1002. PMID 27008860.
   
• Bowman, S.L.*, Soohoo, A.L.*, Shiwarski, D.J., Schulz, S., Pradhan, A.A., Puthenveedu, M.A. Cell autonomous regulation of mu-opioid receptor recycling by substance P. Cell Reports. 2015. 10: 1925-1936. PMID 25801029. (*authors contributed equally to this work).
   
• Soohoo, A.L., Bowersox, S.L., Puthenveedu, M.A. Visualizing clathrin-mediated endocytosis of G protein-coupled receptors at single-event resolution via TIRF microscopy. J Vis Exp. 2014. 92. doi: 10.3791/51805. PMID: 25350161.
   
• Jean-Alphonse, F., Bowersox, S., Chen, S., Beard, G., Puthenveedu, M.A., Hanyaloglu, A.C. Spatially restricted G protein-coupled receptor activity via divergent endocytic compartments. J. Biol. Chem. 2014. 289: 3960-77. PMID: 24375413.

Reviews and Book Chapters

• Bowman, S.L., Puthenveedu, M.A. Postendocytic sorting of adrenergic and opioid receptors: new mechanisms and functions, Prog Mol Biol Transl Sci. 2015. 132: 189-206. doi: 10.1016/bs.pmbts.2015.03.005. PMID: 26055059.
   
• Bowman, S.L., Soohoo, A.L., Puthenveedu, M.A. Visualizing and quantitating sequence-dependent GPCR recycling. In W Guo (Ed.). Methods Cell Biol., Sorting and Recycling Endosomes. 2015. 130: 333-345. doi: 10.1016/bs.mcb.2015.05.007. PMID: 263600.

Contact info:

Shanna L. Bowman, Ph.D.
Children’s Hospital of Philadelphia, Perelman School of Medicine University of Pennsylvania
Abramson Research Center 803
421 Curie Blvd
Philadelphia, PA 19104

Email: bowmansl@email.chop.edu