Robertson Lab

DNA Protocols

The Robertson Lab All Protocols List

	DNA Fragment Purification from Agarose Gel
1	cDNA Amplification Kit
2	CHART RNA
3	DNA Ladder Protocol
4	DNA Max-Preparation Protocol
5	DNA Mini-Preparation Protocol V1
6	DNA Maxi-Preparation. V1 9.06
7	Garbella Cell Gel Assay
8	Gel Shift Assay
9	Hirt supernatent DNA Preparation
10	Mini-Preparation for Plasmid Extraction
11	Mini Preparation Protocol V2
12	Nuclei Paraffin DNA
13	Oligo DNA
14	Optimization of DNA-RNA Extraction from FFPE paper
15	Phenol-Based Methods for Isolating DNA from Agarose Gels
16	Preparation Crude DNA for PCR- Preparation of Phage Lamda DNA
17	Protocols for the in Situ PCR-Ampliﬁcation and Detection of mRNA and DNA Sequences
18	Rapid Separation of Protein-Bound DNA
10	S35 DNA Sequencing
20	Salmon Sperm DNA
21	Simultaneous Purification of Genomic DNA and Total RNA (Supplementary)
22	Trizol Reagent

	DNAse I Footprinting
1	DNAse I protocol for footprinting ToxR membranes
2	Optimization and troubleshooting DNase I footprinting reactions
3	P32 Labeling of Large DNA Fragments

	Phage Lambda DNA Preparation
1	Large Scale Preparation of Bacteriophage Lambda
2	Preparation of Phage Lambda DNA

	Purification by CNBr Coupling Protocol
1	Analysis of DNA-Protein Interactions Using Proteins Synthesized In Vitro from Cloned Genes
2	CNBr-Activated Sepharose 4 Fast Flow
3	CNBr-Activated Sepharose 4B
4	Detection, Purification, and Characterization of cDNA Clones Encoding DNA-Binding Proteins
5	DNA-Protein Interactions
6	DNAse I Footprint Analysis of DNA-Protein Binding
7	Methylation Interference Assay for Analysis of DNA-Protein Interactions
8	Motility Shift DNA-binding Assay Using Gel Electrophoresis
9	Preparation of Nuclear and Cytoplasmic Extracts from Mammalian Cells
10	Purification by CNBr Coupling
11	Purification of DNA-Binding Proteins Using Biotin. Streptavidin Affinity Systems
12	Purification of Sequence-Specific DNA-Binding Proteins by Affinity Chromatography
13	Rapid Separation of Protein-bound DNA From Free DNA Using Nitrocellulose Filters
14	Types of Chromatography
15	UV-Crosslinking of Proteins to Nucleic Acids

	High Efficiency Plasmid Recovery
1	Efficient recovery of plasmid DNA with high nuclease activity
2	High-yield method for DNA isolation