Perelman School of Medicine at the University of Pennsylvania

Robertson Lab

Flow Cytometry & Fluorescence in Situ Hybridization

                                                                       The Robertson Lab All Protocols List
Flow Cytometry
1 Apoptosis Detection with Permeable Cells                                                                           
2 Detection of Apoptosis in Unfixed Samples with Hoechst 33342
3 DFCI Flow Cytometry Facility General Info
4 DNA Staining with Chromomycin A3
5 Hoechst 33342 Staining of Viable Cells
6 Indo 1 Analysis for Calcium Determination
7 Propidium Iodide Staining for Cell Cycle Determination
8 Propidium Iodide Staining for Dead Cell Exclusion
Fluorescence in Situ Hybridization 
1 BAC Fluorescence ISH Single Color                                                                                    
2 FISH Slides
3 FISH with TSA
4 Fluorescence In Situ Hybridization
5 In Situ Detection of RNA and DNA
6 In Situ DNA Hybridization Products
7 Introduction of Micromax Human cDNA Microarray System I
8 One color FISH - Nick translation
9 Standard Protocol
10 Summary of TSA-Direct ISH
11 TSA and In Situ Hybridization
12 TSA Diagrams
13 TSA-Direct Kit Components
14 TSA-Direct Kits