Robertson Lab

Molecular Biology Protocols

The Robertson Lab All Protocols List

	Molecular Biology Protocols
1	Doublestaining Ki-67 CD31 mit EnVision
2	Factors that Influence
3	FICOLL
4	Gelatin Zymography Protocol
5	Gst Pulldown for Proteomic Study
6	Harvesting Cells for Luciferase Assay
7	High-Risk Behaviors Among Adult Men & Women In Botswana: Implications for HIV-AIDS Prevention Efforts
8	Knowledge HIV UB Students
9	Lysis Buffer Preparation of LMP2 Membrane Extracts
10	Montaner CITI
11	Packaging Extract Protocol II
12	Passage of C-terminal XbaI Linker Mutant Virus from E1 C64 N^o 22 LCL
13	Phase Lock Gel Manual
14	Preparation of Vectors Treated with Alkaline Phosphatase
15	Pulse Chase Assay
16	Refractive Index Reference for CsCl
17	Ribonuclease Protection Assay
18	Shift Western Protocol
19	Staph Preparation
20	Stratagene Gigapack Gold Packaging Extract
21	Sucrose Gradient Centrifugation
22	Western Blot of Protein Expressed by Yeast

	P32 Labeling of GST Fusion Proteins
1	Generation of P32-GST Fusing Proteins
2	P32-GST Scale-up Guidelines

	Molecular Cloning Protocols
1	Agar, Agarose and Concentrated Media
2	Antibiotics
3	Autoradiography
4	Buffers for Restriction Endonuclease Digestion
5	Cell Counts with a Hemacytometer
6	Chromatography Through Sephadex G-50
7	Common Electrophoretic Buffers
8	Commonly Used Gel-loading Buffers
9	Concentration of Nucleic Acids
10	Determination of Viability by Trypan Blue Exclusion
11	Drying Down 32P-labeled Nucleotides from Mixtures of Ethanol and Water
12	Enzymes
13	Glassware and Plasticware
14	Liquid Media
15	Mutation Detection by Cleavase Fragment Length Analysis
16	Measurement of Radioactivity in Nucleic Acids
17	Northern Blotting
18	Preparation of Buffers and Solutions
19	Preparation of Dialysis Tubing
20	Preparation of Multimers of Plasmids as Molecular Weight Markers
21	Preparation of Organic Reagents
22	Preparation of Stock Solutions
23	Proteolytic Enzymes
24	Protocol for Sequencing by the Maxam-Gilbert Technique
25	Purification of Nucleic Acids
26	Quantification of DNA and RNA
27	Solutions for Working with Bacteriophage Lambda
28	Solutions Biochemical Techniques

	Preparation of Competent Cells
1	Competent bacteria protocol
2	Preparation of Competent Cells