Perelman School of Medicine at the University of Pennsylvania

Robertson Lab

pFLASH Phagemids & Protein Protocols

                                                                               The Robertson Lab All Protocols List
pFLASH Phagemids Protocols
1 Multifunctional Cat and Luc Reporter Gene Vectors                                                   
2 pFLASH Phagemids Technical Manual
Protein Expression Protocols
1 Amplification Using CHO Cell Expression Vectors
2 Characterization of Recombinant Vaccinia Viruses and Their Products
3 Generation of Recombinant Baculoviruses and Analysis of the Expressed Proteins
4 Generation of Recombinant Vaccinia Viruses
5 Overview of Protein Expression in Mammalian Cells
6 Overview of the Vaccinia Virus Expression System
7 Purification and Transfection of Baculoviral DNA for Generating Recombinant Viruses
8 Purification of Untagged Ubiquitin from Bacterial Cells
9 Transient Expression of Proteins Using COS Cells
10 Protein Interaction Assay by Blotting and Band-Shifting Studies
11 Fractionation of Nuclear or Cytoplasm Proteins
12 Immobilized Protein A
13 Pierce IgG (Protein A) Purification Kit
14 Protein Purification by GST Binding
15 Roche NiNTA protocol
GST-Protein Purification Protocols
1 Glutathione Sepharose 4B Instructions
2 GST Purification
3 Preparation of Large Scale GST-Proteins