Wangensteen Lab

  • Image of liver staining for FAH

    Liver staining for FAH

  • Philadelphia Skyline
  • Immunofluorescence staining

    Immunofluorescence staining showing repopulating hepatocytes (Green label), pericentral hepatocytes (Red), and overlap (Yellow)

Welcome to the Wangensteen Lab

 

Our lab is conducting exciting research on the genetics of liver repopulation and hepatocellular carcinoma (HCC). We have developed two highly innovative in vivo genetic screening paradigms to elucidate genetic pathways involved in carcinogenesis and liver repopulation in the setting of toxic injury. We aim to discover pathways that lead to drug sensitivity and resistance in HCC.  One of our assays involves CRISPR activation screening in vivo in the liver, the first-ever use of this type of screening in live mice.


image depicting in vivo screening approach

 

Announcements

We are hiring!!! We are looking for talented, ambitious scientists who want to make a real impact on human liver diseases.

See our ad for post-doc here

See our ad for research scientist here


September, 2020

Nice to see everyone for a socially-distanced lab lunch at Penn Park. Thanks Adam Zahm for the photography!

Lab lunch at Penn Park

Congrats to Dingzi for two abstracts accepted at the 2020 Liver Meeting

For talk: "A POOLED IN VIVO CRISPRI SCREEN IDENTIFIES SYNTHETIC LETHAL PARTNERS OF MYC IN HEPATOCELLULAR CARCINOMA DEVELOPMENT"

For poster: "AN IN VIVO DROPOUT CRISPRI SCREEN FOR REGULATORS OF LIVER REGENERATION" 

August, 2020

Check out our new review in Hepatology on the multiple roles for hepatitis B and C in driving liver cancer.

July, 2020

Welcome Markcus Lee, new research specialist in the lab!

Bye-bye to Noam, who is return to his home in Israel. Thank you for the many years of hard work and dedication to the lab! We will miss you!

Congrats to Amber on her new position at Genentech!

May, 2020

Congratulations Ale on passing the PhD prelim exam! Well done!

March, 2020

Congrats to Morgan on taking a job at Chimeron. We will miss you!

Congrats Dingzi on his new baby!

In press, editorial by Yin, Dingzi and Wangensteen, KJ, "Incapacitated Capicua in sorafenib-resistant HCC" at Cellular and Molecular Gastroenterology and Hepatology

Nov, 2019:

Now published, the largest ever in vivo screen, conducted by Adam Zahm and colleagues to identify miRNA interactions in liver repopulation. This paper has beautiful graphics to depict the technology and results. Check it out:

Zahm AM, Wang AW, Wang YJ, Schug, J, Wangensteen KJ*, Kaestner KH*. High-content Screen Identifies MicroRNAs Required for Liver Repopulation After Injury in Mice. Gastroneterology. 2019. In press. * co-corresponding.

 

Wangensteen Lab had a strong showing at the 2019 AASLD Liver meeting!

  • Congrats Morgan on receiving the Professor Margit Hamosh Memorial Travel Award for the most outstanding abstract on hepatocellular carcinoma research for The Liver Meeting for her abstract entitled, "LXR agonism potentiates Sorafenib activity in HCC by inducing metabolic stress". She gave an excellent oral presentation.
  • Congrats Julia Kieckhaefer for presenting her poster of distinction, "Identification of effectors of TNFA signaling that modulate liver repopulation by high throughput CRISPRA screening," which received a lot of interest.

Oct, 2019: 

Congratulations Amber on publishing this interesting work to examine the changes in chromatin in the repopulating liver:

Wang AW, Wang YJ, Zahm AM, Morgan AR, Wangensteen KJ, Kaestner KH. The Dynamic Chromatin Architecture of the Regenerating Liver. Cell Mol Gastroenterol Hepatol. 2019 Oct 17.

 

Wangensteen Lab presents at the 2019 Penn Post-doc symposium:

Sept, 2019:

Congratations Amber Wang, PhD, on a wonderful PhD thesis defense!

The Wangensteen lab welcomes Master's in Translational Medicine trainee Jenna Mancinelli!

Our paper, "Cell Type-Specific Gene Expression Profiling in the Mouse Liver" is now published at JoVE

 

June, 2019:

The Wangensteen Lab welcomes graduate student Alexandra M. Vázquez Salgado!

Congratulations Kirk Wangensteen for being named a Beckman Young Investigator!

Congratulations Kirk Wangensteen for being selected for the Gastroenterology Faculty Research Award!

May, 2019:

Thank you Jie for your hard work this year in the Wangensteen Lab! We will miss you!

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