Kathryn E. Hamilton, PhD

faculty photo
Assistant Professor of Pediatrics
Department: Pediatrics
Graduate Group Affiliations

Contact information
902F Abramson Research Center
3615 Civic Center Blvd
Children's Hospital of Philadelphia
Philadelphia, PA 19104
Lab: 267-426-5266
Education:
BA (Biology)
Assumption College, 2003.
PhD (Cell and Molecular Physiology)
University of North Carolina Chapel Hill, 2010.
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Description of Research Expertise

Research Interests: The Hamilton laboratory specializes in understanding mechanisms regulating gut homeostasis in health and disease, with a specific interest in epithelial cell responses to damage and inflammation. We employ in vivo models and primary, three-dimensional enteroid and colonoid cultures from mice and humans to understand the molecular basis of Inflammatory Bowel Disease (IBD) and colorectal cancer. Within these contexts, we are interested in how RNA binding proteins impart global, post-transcriptional regulation of key pathways in normal physiology and pathophysiology.

Keywords: gastrointestinal biology, RNA binding proteins, Inflammatory Bowel Disease (IBD), colorectal cancer, organoids (enteroids/colonoids), in vivo models

Research Details: Our laboratory incorporates training in cell biology, physiology/pathophysiology, and molecular genetics to understand basic mechanisms in human disease, providing foundations for new biomarkers or therapeutic targets. Techniques employed in the laboratory include tissue culture (2D and 3D), molecular biology, microscopy, immunohistochemistry, flow cytometry and in vivo disease models. New techniques being incorporated into the laboratory in the coming months include transcriptomics, ribonomics, and proteomics to understand global changes in the context of deleting or overexpressing RNA binding proteins.

Rotation Projects:
1) Dissecting regulation of the autophagy pathway by the RNA binding protein IMP1 in 3D colonoids
2) Evaluating specific inflammatory factors regulating the RNA binding protein IMP1 and response to experimental colitis
3) Determining RNA binding protein "regulons" in adult Inflammatory Bowel Disease
4) Evaluating novel pathways regulated by IMP1 in pediatric, VEO (Very Early Onset)-IBD
5) Evaluating the role of RNA binding protein IMP1 overexpression in amplifying or suppressing key oncogenes in the progression of inflammation-associated colorectal cancer

Lab Personnel:
Lauren Simon, BS, Laboratory manager
Rithika Behera, BS, Penn Master's in Biotechnology student
Laura Bryant, PhD, Postdoctoral fellow
Patrick Williams, PhD, Postdoctoral fellow
Louis Parham, BS, CAMB/Cancer Biology PhD rotation student

Selected Publications

Kelsen JR, Conrad MA, Simon LA, Williams KN, Mizuno R, Chatterji P, Dawany N, Giraudo C, Behrens EM, Sullivan KE, Devoto M, Hamilton KE : Using functional assays in patient-derived enteroids to understand genotype-phenotype interactions in Very Early Onset Inflammatory Bowel Disease (VEO-IBD) (Poster Presentation) Digestive Diseases Week May 2017 Notes: Chicago, IL.

Dana Almohazey, Yuan-Hung Lo, Claire Vossler, Alan Simmons, Jonathan Hsieh, Edie Bucar, Michael Schumacher, Kathryn Hamilton, Ken Lau, Noah Shroyer, Mark Frey: The ErbB3 receptor tyrosine kinase negatively regulates Paneth cells by PI3K-dependent suppression of Atoh1. Cell Death and Differentiation March 2017.

VĂ©ronique Giroux, Ashley A. Lento, Mirazul Islam, Jason R. Pitarresi, Akriti Kharbanda, Kathryn E. Hamilton, Kelly A. Whelan, Apple Long, Ben Rhoades, Qiaosi Tang, Hiroshi Nakagawa, Christopher J. Lengner, Adam J. Bass, E. Paul Wileyto, Andres J. Klein-Szanto, Timothy C. Wang, and Anil K. Rustgi: Long-lived keratin 15+ esophageal progenitor cells contribute to homeostasis and regeneration. Journal of Clinical Investigation May 2017.

Kelly A Whelan, Jamie F Merves, Veronique Giroux, Koji Tanaka, Andy Guo,Prasanna M Chandramouleeswaran,, Alain J Benitez, Kara Dods, Jianwen Que, Joanne C Masterson, Shahan D Fernando, Bridget C Godwin, Andres J Klein-Szanto, Kudakwashe Chikwava, Eduardo D Ruchelli, Kathryn E Hamilton, Amanda B Muir, Mei-Lun Wang, Glenn T Furuta, Gary W Falk, Jonathan M Spergel, Hiroshi Nakagawa: Autophagy mediates epithelial cytoprotection in eosinophilic oesophagitis. Gut February 2016 Notes: doi: 10.1136/gutjnl-2015-310341. [Epub ahead of print]

Kathryn E. Hamilton, Priya Chatterji, Emma T. Lundsmith, Sarah F. Andres, Veronique Giroux, Philip D. Hicks, Felicite K. Noubissi, Vladimir S. Spiegelman, Anil K. Rustgi: Loss of Stromal IMP1 Promotes a Tumorigenic Microenvironment in the Colon. Molecular Cancer Research 13(11): 1478-86, November 2015.

Hamilton Kathryn E, Crissey Mary Ann S, Lynch John P, Rustgi Anil K: Culturing adult stem cells from mouse small intestinal crypts. Cold Spring Harbor protocols 2015(4): 354-8, April 2015.

Ravi K. Amaravadi, Kathryn E. Hamilton, Xiaohong Ma, Shengfu Piao, Armando Del Portillo, Katherine L. Nathanson, Matteo S. Carlino, Georgina V. Long, Igor Puzanov, Xiaowei Xu, Jennifer J.D. Morrissette, Kenneth Y. Tsai, Keith T. Flaherty, Jeffrey A. Sosman, Grant R. Goodman, Grant A. McArthur, Anil K. Rustgi, David C. Metz, Lynn M. Schuchter, Paul B. Chapman, and Antonia R. Sepulveda: Multiple Gastrointestinal Polyps in Patients Treated with BRAF Inhibitors. Clinical Cancer Research 21(23): 5215-21, December 2015.

Kathryn E. Hamilton Felicite K. Noubissi Prateek S. Katti Christopher M. Hahn Sonya R. Davey Emma T. Lundsmith Andres J. Klein-Szanto Andrew D. Rhim Vladimir S. Spiegelman Anil K. Rustgi: IMP1 promotes tumor growth, dissemination and a tumor-initiating cell phenotype in colorectal cancer cell xenografts. Carcinogenesis 34(11): 2647-54, November 2013.

Kathryn E. Hamilton, James G. Simmons, Shengli Ding, Laurianne Van Landeghem, P. Kay Lund : Cytokine induction of tumor necrosis factor receptor 2 is mediated by STAT3 in colon cancer cells. Molecular Cancer Research 9(12): 1718-31, December 2011.

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Last updated: 06/05/2018
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