Kathryn E. Hamilton, PhD

faculty photo
Assistant Professor of Pediatrics
Department: Pediatrics
Graduate Group Affiliations

Contact information
902F Abramson Research Center
3615 Civic Center Blvd
Children's Hospital of Philadelphia
Philadelphia, PA 19104
Lab: 267-426-5266
Education:
BA (Biology)
Assumption College, 2003.
PhD (Cell and Molecular Physiology)
University of North Carolina Chapel Hill, 2010.
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Description of Research Expertise

Research Interests: The Hamilton laboratory specializes in understanding mechanisms regulating gut homeostasis in health and disease, with a specific interest in epithelial cell responses to damage and inflammation. We employ in vivo models and primary, three-dimensional enteroid and colonoid cultures from mice and humans to understand the molecular basis of inflammatory bowel disease (IBD) and colorectal cancer. Within these contexts, we are interested in how RNA binding proteins impart global, post-transcriptional regulation of key pathways in normal physiology and pathophysiology.

Keywords: gastrointestinal biology, RNA binding proteins, inflammatory bowel disease (IBD), colorectal cancer, organoids (enteroids/colonoids), in vivo models

Research Details: Our laboratory incorporates training in cell biology, physiology/pathophysiology, and molecular genetics to understand basic mechanisms in human disease, providing foundations for new biomarkers or therapeutic targets. Techniques employed in the laboratory include tissue culture (2D and 3D), molecular biology, microscopy, immunohistochemistry, flow cytometry and in vivo disease models. New techniques being incorporated into the laboratory in the coming months include transcriptomics, ribonomics, and proteomics to understand global changes in the context of deleting or overexpressing RNA binding proteins.

Rotation Projects:
1) Dissecting regulation of the autophagy pathway by the RNA binding protein IMP1 in 3D colonoids
2) Evaluating specific inflammatory factors regulating the RNA binding protein IMP1 and response to experimental colitis
3) Determining RNA binding protein "regulons" in adult Inflammatory Bowel Disease
4) Evaluating novel pathways regulated by IMP1 in pediatric, VEO (Very Early Onset)-IBD
5) Evaluating the role of RNA binding protein IMP1 overexpression in amplifying or suppressing key oncogenes in the progression of inflammation-associated colorectal cancer

Lab Personnel:
Lauren Simon, BS, Laboratory manager
Rithika Behera, BS, Penn Master's in Biotechnology student
Laura Bryant, PhD, Postdoctoral fellow
Patrick Williams, PhD, Postdoctoral fellow
Louis Parham, BS, CAMB/Cancer Biology PhD student
Maiah Dent, BS, Research specialist
Gabrielle Largoza, summer undergraduate research student
Michael Sun, summer undergraduate research student

Selected Publications

Priya Chatterji*, Kathryn E. Hamilton*, Shun Liang, Sarah F. Andres, H. R. Sagara Wijeratne, Rei Mizuno, Lauren A. Simon, Philip D. Hicks, Shawn W. Foley, Jason R. Pitarresi, Andres J. Klein-Szanto, Amanda T. Mah, Laurianne Van Landeghem, Brian D. Gregory, Christopher J. Lengner, Blair B. Madison, Premal Shah, and Anil K. Rustgi *co-first author: The LIN28B-IMP1 post-transcriptional regulon has opposing effects on oncogenic signaling in the intestine. Genes and Development In press, August 2018.

Véronique Giroux, Julien Stephan, Priya Chatterji, Ben Rhoades, Andres J. Klein-Szanto, Christopher J. Lengner, Kathryn E. Hamilton, Anil K. Rustgi: Mouse intestinal krt15+ crypt cells are radio-resistant and tumor-initiating. Stem Cell Reports 10(6), June 2018.

Rei Mizuno, Priya Chatterji, Sarah F. Andres, Kathryn E. Hamilton, Lauren A. Simon, Shawn Foley, Arjun Jeganathan, Brian Gregory, Blair B. Madison, and Anil K. Rustgi : Differential regulation of LET-7 by LIN28B isoform-specific functions. Molecular Cancer Research 16(3), March 2018.

Yuta Kasagia, Prasanna M. Chandramouleeswarana, Kelly A. Whelan, Koji Tanaka, Veronique Giroux, Medha Sharma, Joshua Wang, Alain J. Benitez, Maureen DeMarshall, John W. Tobias, Kathryn E. Hamilton, Gary W. Falk, Jonathan M. Spergel, Andres J. Klein-Szanto, Anil K. Rustgi, Amanda B. Muirb, Hiroshi Nakagawa: The esophageal organoid system reveals functional interplay between Notch and cytokines in reactive epithelial changes. Cell and Molecular Gastroenterology 5(3), January 2018.

Kelly A Whelan, Jamie F Merves, Veronique Giroux, Koji Tanaka, Andy Guo,Prasanna M Chandramouleeswaran,, Alain J Benitez, Kara Dods, Jianwen Que, Joanne C Masterson, Shahan D Fernando, Bridget C Godwin, Andres J Klein-Szanto, Kudakwashe Chikwava, Eduardo D Ruchelli, Kathryn E Hamilton, Amanda B Muir, Mei-Lun Wang, Glenn T Furuta, Gary W Falk, Jonathan M Spergel, Hiroshi Nakagawa: Autophagy mediates epithelial cytoprotection in eosinophilic oesophagitis. Gut 66(7), July 2017.

Véronique Giroux, Ashley A. Lento, Mirazul Islam, Jason R. Pitarresi, Akriti Kharbanda, Kathryn E. Hamilton, Kelly A. Whelan, Apple Long, Ben Rhoades, Qiaosi Tang, Hiroshi Nakagawa, Christopher J. Lengner, Adam J. Bass, E. Paul Wileyto, Andres J. Klein-Szanto, Timothy C. Wang, and Anil K. Rustgi: Long-lived keratin 15+ esophageal progenitor cells contribute to homeostasis and regeneration. Journal of Clinical Investigation 127(6), June 2017.

Dana Almohazey, Yuan-Hung Lo, Claire Vossler, Alan Simmons, Jonathan Hsieh, Edie Bucar, Michael Schumacher, Kathryn Hamilton, Ken Lau, Noah Shroyer, Mark Frey: The ErbB3 receptor tyrosine kinase negatively regulates Paneth cells by PI3K-dependent suppression of Atoh1. Cell Death and Differentiation 24(5), May 2017.

Ravi K. Amaravadi, Kathryn E. Hamilton, Xiaohong Ma, Shengfu Piao, Armando Del Portillo, Katherine L. Nathanson, Matteo S. Carlino, Georgina V. Long, Igor Puzanov, Xiaowei Xu, Jennifer J.D. Morrissette, Kenneth Y. Tsai, Keith T. Flaherty, Jeffrey A. Sosman, Grant R. Goodman, Grant A. McArthur, Anil K. Rustgi, David C. Metz, Lynn M. Schuchter, Paul B. Chapman, and Antonia R. Sepulveda: Multiple gastrointestinal polyps in patients treated with BRAF inhibitors. Clinical Cancer Research 21(23), December 2015.

Kathryn E. Hamilton, Priya Chatterji, Emma T. Lundsmith, Sarah F. Andres, Veronique Giroux, Philip D. Hicks, Felicite K. Noubissi, Vladimir S. Spiegelman, Anil K. Rustgi: Loss of stromal IMP1 promotes a tumorigenic microenvironment in the colon. Molecular Cancer Research 13(11), November 2015.

Long Apple, Giroux Véronique, Whelan Kelly A, Hamilton Kathryn E, Tétreault Marie-Pier, Tanaka Koji, Lee Ju-Seog, Klein-Szanto Andres J, Nakagawa Hiroshi, Rustgi Anil K: WNT10A promotes an invasive and self-renewing phenotype in esophageal squamous cell carcinoma. Carcinogenesis 36(5), May 2015.

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Last updated: 09/21/2018
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