Rajan Jain, MD

faculty photo
Assistant Professor of Medicine
Department: Medicine
Graduate Group Affiliations

Contact information
09-102 Smilow Translational Research Building
3400 Civic Center Blvd
Philadelphia, PA 19104
Office: 215-746-5732
Lab: 215-573-3011
Education:
BA (Molecular and Cellular Biology)
University of California, Berkeley, 2001.
MD
New York University School of Medicine, 2005.
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Description of Research Expertise

Research Interests: We are interested in how genome organization shapes organogenesis.

Keywords: Nuclear architecture, nuclear lamina, chromatin, epigenetics, genome organization, heart development, stem cell biology

Research Details:
An overarching goal is to understand cell identity is established and maintained. We combine traditional molecular biology and genetic tools with innovative imaging and next generation sequencing approaches to define how an embryonic stem cell progressively restrict their potential to give rise to various differentiated cell types in the body. We also routinely leverage classic models of stem cell biology to dissect the underpinnings of cell fate decisions. We have a focus on cardiac biology, but routinely work outside it.

It has become clear that the genome is folded and organized in a stereotypical manner in three- dimensional space. A major interest of the laboratory is deciphering how the organization of the genome in 3D space regulates genes and cell fate decisions. We are defining how the nuclear lamina, a network of proteins on the inner side of the nuclear membrane, interacts with chromatin to drive gene regulation and shape cell fate choices. Projects in the laboratory are geared towards defining the rules that govern nuclear lamina-chromatin interactions. We are also differentiating human embryonic stem cells into cardiac myocytes to study how mutations in LMNA and related proteins impact nuclear architecture and translate into cardiac dysfunction in patients.

A second, complementary, focus in the laboratory is defining how cell identity is established after mitosis. After each round of mitosis, gene programs must be precisely re-established in order to preserve cell identity. However, the mechanisms underlying this process during cardiac development are not well understood. We are trying to decipher the factors and mechanisms responsible for establishing cell identity after a cardiac cell divides.

Lab Personnel:
Rajan Jain
Nicholas Rizer
Parisha Shah
Qiaohong Wang

Selected Publications

Ramjee Vimal, Li Deqiang, Manderfield Lauren J, Liu Feiyan, Engleka Kurt A, Aghajanian Haig, Rodell Christopher B, Lu Wen, Ho Vivienne, Wang Tao, Li Li, Singh Anamika, Cibi Dasan M, Burdick Jason A, Singh Manvendra K, Jain Rajan, Epstein Jonathan A: Epicardial YAP/TAZ orchestrate an immunosuppressive response following myocardial infarction. The Journal of clinical investigation 127(3): 899-911, Mar 2017.

Bardot Evan, Calderon Damelys, Santoriello Francis, Han Songyan, Cheung Kakit, Jadhav Bharati, Burtscher Ingo, Artap Stanley, Jain Rajan, Epstein Jonathan, Lickert Heiko, Gouon-Evans Valerie, Sharp Andrew J, Dubois Nicole C: Foxa2 identifies a cardiac progenitor population with ventricular differentiation potential. Nature communications 8: 14428, Feb 2017.

Loh Kyle M, Chen Angela, Koh Pang Wei, Deng Tianda Z, Sinha Rahul, Tsai Jonathan M, Barkal Amira A, Shen Kimberle Y, Jain Rajan, Morganti Rachel M, Shyh-Chang Ng, Fernhoff Nathaniel B, George Benson M, Wernig Gerlinde, Salomon Rachel E A, Chen Zhenghao, Vogel Hannes, Epstein Jonathan A, Kundaje Anshul, Talbot William S, Beachy Philip A, Ang Lay Teng, Weissman Irving L: Mapping the Pairwise Choices Leading from Pluripotency to Human Bone, Heart, and Other Mesoderm Cell Types. Cell 166(2): 451-67, Jul 2016.

Jain R*, Li D*, Gupta M, Manderfield LJ, Ifkovits JL, Wang Q, Liu F, Liu Y, Poleshko A, Padmanabhan A, Raum JC, Li L, Morrisey EE, Lu MM, Won KJ, and Epstein JA: Integration of Bmp and Wnt signaling by Hopx specifies commitment of cardiomyoblasts. Science 348(6242), June 2015.

Jain R*, Barkauskas CE*, Takeda N, Bowie EJ, Aghajanian H, Wang Q, Padmanabhan A, Manderfield LJ, Gupta M, Li D, Li L, Trivedi CM, Hogan BLM, Epstein JA: Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung. Nature Communications 6: 6727, April 2015.

Li N, Yousefi M, Nakauka-Ddamba A, Jain R, Tobias J, Epstein JA, Jensen ST, Lengner CJ: Single-cell analysis of proxy reporter allele-marked epithelial cells establishes intestinal stem cell hierarchy. Stem Cell Reports 3(5): 876-91, November 2014 Notes: Epub 2014 Oct 23.

Takeda N*, Jain R*, Leboeuf MR, Padmanabhan A, Wang Q, Li L, Lu MM, Millar SE, Epstein JA: Hopx expression defines a subset of multipotent hair follicle stem cells and a progenitor population primed to give rise to K6+ niche cells. Development 140(8): 1655-64, April 2013 Notes: *co-first author - Epub 2013 Mar 13.

Takeda N*, Jain R*, LeBoeuf MR, Wang Q, Lu MM, Epstein JA: Interconversion between intestinal stem cell populations in distinct niches. Science 334(6061): 1420-4, December 2011 Notes: *co-first author - Epub 2011 Nov 10.

Jain R, Engleka KA, Rentschler SL, Manderfield LJ, Li L, Yuan L, Epstein JA: Cardiac neural crest orchestrates remodeling and functional maturation of mouse semilunar valves. Journal of Clinical Investigation 121(1): 422-30, January 2011 Notes: Epub 2010 Dec 13.

High FA*, Jain R*, Stoller JZ, Antonucci NB, Lu MM, Loomes KM, Kaestner KH, Pear WS, Epstein JA: Murine Jagged1/Notch signaling in the second heart field orchestrates Fgf8 expression and tissue-tissue interactions during outflow tract development. Journal of Clinical Investigation 119(7): 1986-96, July 2009 Notes: *co-first author - Epub 2009 Jun 8.

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Last updated: 09/14/2017
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