Rajan Jain, MD

faculty photo
Assistant Professor of Medicine
Department: Medicine
Graduate Group Affiliations

Contact information
09-102 Smilow Translational Research Building
3400 Civic Center Blvd
Philadelphia, PA 19104
Office: 215-746-5732
Lab: 215-573-3011
BA (Molecular and Cellular Biology)
University of California, Berkeley, 2001.
New York University School of Medicine, 2005.
Permanent link

Description of Research Expertise

Research Interests: We are interested in how genome organization shapes organogenesis.

Keywords: Nuclear architecture, gene regulation, chromatin, epigenetics, 3D genome organization, heart development, stem cell biology

Research Details:
An overarching goal is to understand cell identity is established and maintained. We combine traditional molecular biology and genetic tools with innovative imaging and next-generation sequencing approaches to define how an embryonic stem cell progressively restrict their potential to give rise to various differentiated cell types in the body. We also routinely leverage classic models of stem cell biology to dissect the underpinnings of cell fate decisions. We have a focus on cardiac biology but routinely work outside it.

It has become clear that the genome is folded and organized in a stereotypical manner in three- dimensional space. A major interest of the laboratory is deciphering how the organization of the genome in 3D space regulates genes and cell fate decisions. We are defining how the nuclear lamina, a network of proteins on the inner side of the nuclear membrane, interacts with chromatin to drive gene regulation and shape cell fate choices. Projects in the laboratory are geared towards defining the rules that govern nuclear lamina-chromatin interactions. We are also studying how mutations in LMNA and related proteins impact nuclear architecture and translate into cardiac dysfunction in patients.

Lab Personnel:
Rajan Jain
Ricardo Llinares Saldana
Nicholas Rizer
Parisha Shah
Qiaohong Wang

Selected Publications

Poleshko, A.*, Shah, P. P.*, Gupta, M.*, Babu, A., Morley, M. P., Manderfield, L. J., Ifkovits, J. L., Calderon, D., Aghajanian, H., Sierra-Pagan, J. E., Sun, Z., Wang, Q., Li, L., Dubois, N. C., Morrisey, E. E., Lazar, M. A., Smith, C. L., Epstein, J. A.**, Jain, R.**: Genome-Nuclear Lamina Interactions Regulate Cardiac Stem Cell Lineage Restriction. Cell 171(3): 573-587 e14, 2017.

Aghajanian, H., Cho, Y. K., Rizer, N. W., Wang, Q., Li, L., Degenhardt, K., Jain, R.: Pdgfralpha functions in endothelial-derived cells to regulate neural crest cells and the development of the great arteries. Dis Model Mech 10(9): 1101-1108, 2017.

Ramjee, V., Li, D., Manderfield, L. J., Liu, F., Engleka, K. A., Aghajanian, H., Rodell, C. B., Lu, W., Ho, V., Wang, T., Li, L., Singh, A., Cibi, D. M., Burdick, J. A., Singh, M. K.*, Jain, R.*, Epstein, J. A.*: Epicardial YAP/TAZ orchestrate an immunosuppressive response following myocardial infarction. J Clin Invest 127(3): 899-911, 2017 Notes: *co-corresponding author.

Kook, H., Seo, S. B., Jain, R.: EZ Switch From EZH2 to EZH1: Histone Methylation Opens a Window of Cardiac Regeneration. Circ Res 121(2): 91-94, 2017.

Loh, K. M., Chen, A., Koh, P. W., Deng, T. Z., Sinha, R., Tsai, J. M., Barkal, A. A., Shen, K. Y., Jain, R., Morganti, R. M., Shyh-Chang, N., Fernhoff, N. B., George, B. M., Wernig, G., Salomon, R. E. A., Chen, Z., Vogel, H., Epstein, J. A., Kundaje, A., Talbot, W. S., Beachy, P. A., Ang, L. T., Weissman, I. L.: Mapping the Pairwise Choices Leading from Pluripotency to Human Bone, Heart, and Other Mesoderm Cell Types. Cell 166(2): 451-467, 2016.

Jain, R.*, Li, D.*, Gupta, M., Manderfield, L. J., Ifkovits, J. L., Wang, Q., Liu, F., Liu, Y., Poleshko, A., Padmanabhan, A., Raum, J. C., Li, L., Morrisey, E. E., Lu, M. M., Won, K. J., Epstein, J. A.: Integration of Bmp and Wnt signaling by Hopx specifies commitment of cardiomyoblasts. Science 348(6242): aaa6071, 2015.

Jain, R.*, Barkauskas, C. E.*, Takeda, N., Bowie, E. J., Aghajanian, H., Wang, Q., Padmanabhan, A., Manderfield, L. J., Gupta, M., Li, D., Li, L., Trivedi, C. M., Hogan, B. L., Epstein, J. A.: Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung. Nat Commun 6: 6727, 2015.

Takeda, N.*, Jain, R.*, Leboeuf, M. R., Padmanabhan, A., Wang, Q., Li, L., Lu, M. M., Millar, S. E., Epstein, J. A.: Hopx expression defines a subset of multipotent hair follicle stem cells and a progenitor population primed to give rise to K6+ niche cells. Development 140(8): 1655-64, 2013 Notes: co-first author.

Takeda, N.*, Jain, R.*, LeBoeuf, M. R., Wang, Q., Lu, M. M., Epstein, J. A.: Interconversion between intestinal stem cell populations in distinct niches. Science 334(6061): 1420-4, 2011 Notes: *co-first author.

Jain, R., Engleka, K. A., Rentschler, S. L., Manderfield, L. J., Li, L., Yuan, L., Epstein, J. A.: Cardiac neural crest orchestrates remodeling and functional maturation of mouse semilunar valves. J Clin Invest 121(1): 422-30, 2011.

High, F. A.*, Jain, R.*, Stoller, J. Z., Antonucci, N. B., Lu, M. M., Loomes, K. M., Kaestner, K. H., Pear, W. S., Epstein, J. A.: Murine Jagged1/Notch signaling in the second heart field orchestrates Fgf8 expression and tissue-tissue interactions during outflow tract development. J Clin Invest 119(7): 1986-96, 2009 Notes: *co-first author.

Ladurner, A. G., Inouye, C., Jain, R., Tjian, R.: Bromodomains mediate an acetyl-histone encoded antisilencing function at heterochromatin boundaries. Mol Cell 11(2): 365-76, 2003.

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Last updated: 10/22/2017
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