Rumela Chakrabarti, Ph.D.

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Graduate Group Affiliations

Contact information

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Education:
BS (Zoology)
National Institute of Immunology, New Delhi, India, 2000.
MS (Genetics)
University of Pune, India, 2001.
PhD (Cellular & Molecular Biology)
Kent State University, 2007.
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Description of Research Expertise

1. We seek to determine how cell intrinsic function of transcription factors dictating cell fate affect normal mammary stem/progenitor cells in mammary gland development as well as cancer stem cells in breast cancer.

2. Unlike other organs such as skin and intestine, mammary gland stem cell niche still remain elusive. Our laboratory is interested in identifying the stem cell niche cells in normal mammary gland as well as in breast cancer. Particularly, we are interested in understanding function and mechanism of how cells such as macrophages, fibroblasts and endothelial cells affect stem cells in normal tissue development and in disease such as breast cancer. We will use mouse genetics, lineage tracing, 2-photon microscopy, in vitro co-culture assays, bioinformatics and other molecular biology assays to determine the functions of microenvironment on stem cells in normal development of breast and in breast cancer.

3. It is known that the behavior of the initial transformed cells after oncogenic insult can dictate the fate of the tumor cells during disease progression. However, cell-of-origin of breast cancer is still poorly understood. We aim to characterize the cell of origin/tumor initiating cells in different subtypes of breast cancer using powerful tools like lineage tracing, whole mount mammary gland 3-D imaging and mouse genetics.

4. Determine how epigenetic modification such as histone modification and/or DNA methylation control fate of normal stem cells and cancer stem cells.

Selected Publications

Guha, M., Srinivasan, S., Raman, P., Jiang, Y., Kaufman, B. A., Taylor, D., Dong, D., Chakrabarti, R., Picard, M., Carstens, R. P., Kijima, Y., Feldman, M., Avadhani, N. G.: Aggressive triple negative breast cancers have unique molecular signature on the basis of mitochondrial genetic and functional defects. Biochim Biophys Acta 1864(4 Pt A): 1060-1071, 2018.

Celia-Terrassa, T., Liu, D. D., Choudhury, A., Hang, X., Wei, Y., Zamalloa, J., Alfaro-Aco, R., Chakrabarti, R., Jiang, Y. Z., Koh, B. I., Smith, H. A., DeCoste, C., Li, J. J., Shao, Z. M., Kang, Y.: Normal and cancerous mammary stem cells evade interferon-induced constraint through the miR-199a-LCOR axis. Nat Cell Biol 19(6): 711-723, 2017.

Chakrabarti R, Kang Y: Transplantable mouse tumor models of breast cancer metastasis. Methods in molecular biology (Clifton, N.J.) 1267: 367-80, 2015.

Mathias RA, Greco TM, Oberstein A, Budayeva HG, Chakrabarti R, Rowland EA, Kang Y, Shenk T, Cristea IM: Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity. Cell 159(7): 1615-25, Dec 2014.

Chakrabarti R, Wei Y, Hwang J, Hang X, Andres Blanco M, Choudhury A, Tiede B, Romano RA, DeCoste C, Mercatali L, Ibrahim T, Amadori D, Kannan N, Eaves CJ, Sinha S, Kang Y: ΔNp63 promotes stem cell activity in mammary gland development and basal-like breast cancer by enhancing Fzd7 expression and Wnt signalling. Nature cell biology 16(10): 1004-15, 1-13, Oct 2014.

Wan L, Lu X, Yuan S, Wei Y, Guo F, Shen M, Yuan M, Chakrabarti R, Hua Y, Smith HA, Blanco MA, Chekmareva M, Wu H, Bronson RT, Haffty BG, Xing Y, Kang Y: MTDH-SND1 interaction is crucial for expansion and activity of tumor-initiating cells in diverse oncogene- and carcinogen-induced mammary tumors. Cancer cell 26(1): 92-105, Jul 2014.

Cicchini M, Chakrabarti R, Kongara S, Price S, Nahar R, Lozy F, Zhong H, Vazquez A, Kang Y, Karantza V: Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity. Autophagy 10(11): 2036-52, 2014.

Chakrabarti R, Hwang J, Andres Blanco M, Wei Y, Lukačišin M, Romano RA, Smalley K, Liu S, Yang Q, Ibrahim T, Mercatali L, Amadori D, Haffty BG, Sinha S, Kang Y: Elf5 inhibits the epithelial-mesenchymal transition in mammary gland development and breast cancer metastasis by transcriptionally repressing Snail2. Nature cell biology 14(11): 1212-22, Nov 2012.

Chakrabarti R, Wei Y, Romano RA, DeCoste C, Kang Y, Sinha S: Elf5 regulates mammary gland stem/progenitor cell fate by influencing notch signaling. Stem cells (Dayton, Ohio) 30(7): 1496-508, Jul 2012.

Yusuf D, Butland SL, Swanson MI, Bolotin E, Ticoll A, Cheung WA, Zhang XY, Dickman CT, Fulton DL, Lim JS, Schnabl JM, Ramos OH, Vasseur-Cognet M, de Leeuw CN, Simpson EM, Ryffel GU, Lam EW, Kist R, Wilson MS, Marco-Ferreres R, Brosens JJ, Beccari LL, Bovolenta P, Benayoun BA, Monteiro LJ, Schwenen HD, Grontved L, Wederell E, Mandrup S, Veitia RA, Chakravarthy H, Hoodless PA, Mancarelli MM, Torbett BE, Banham AH, Reddy SP, Cullum RL, Liedtke M, Tschan MP, Vaz M, Rizzino A, Zannini M, Frietze S, Farnham PJ, Eijkelenboom A, Brown PJ, Laperrière D, Leprince D, de Cristofaro T, Prince KL, Putker M, del Peso L, Camenisch G, Wenger RH, Mikula M, Rozendaal M, Mader S, Ostrowski J, Rhodes SJ, Van Rechem C, Boulay G, Olechnowicz SW, Breslin MB, Lan MS, Nanan KK, Wegner M, Hou J, Mullen RD, Colvin SC, Noy PJ, Webb CF, Witek ME, Ferrell S, Daniel JM, Park J, Waldman SA, Peet DJ, Taggart M, Jayaraman PS, Karrich JJ, Blom B, Vesuna F, O'Geen H, Sun Y, Gronostajski RM, Woodcroft MW, Hough MR, Chen E, Europe-Finner GN, Karolczak-Bayatti M, Bailey J, Hankinson O, Raman V, LeBrun DP, Biswal S, Harvey CJ, DeBruyne JP, Hogenesch JB, Hevner RF, Héligon C, Luo XM, Blank MC, Millen KJ, Sharlin DS, Forrest D, Dahlman-Wright K, Zhao C, Mishima Y, Sinha S, Chakrabarti R, Portales-Casamar E, Sladek FM, Bradley PH, Wasserman WW: The transcription factor encyclopedia. Genome biology 13(3): R24, 2012.

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Last updated: 02/13/2018
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