Michael Ostap, Ph.D.

faculty photo
Professor of Physiology
Department: Physiology

Contact information
Pennsylvania Muscle Institute &
Department of Physiology
700A Clinical Research Building
415 Curie Blvd
Philadelphia, PA 19104
Office: (215) 573-9758
Lab: (215) 898-3685
Education:
B.S. (Chemistry)
Illinois State University, 1988.
Ph.D. (Biochemistry, Molecular Biology, and Biophysics)
University of Minnesota, 1993.
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Description of Research Expertise

Research Interests
Molecular Motors, Cell Motility, Mechanosensing, Single-Molecule Biophysics, Biochemistry

Key words: Cell motility, myosin, actin, biochemistry, biophysics, single molecule, spectroscopy, microscopy, fluorescence, nanotechnology.

Description of Research
The goal of our research is to understand the molecular mechanisms of cell motility, which include: cell migration, regulation of cell shape and extracellular attachment, and intracellular transport. Cell motility is crucial for several normal and pathological processes, including: cell and tissue development, endocytosis, wound healing, immune response, and metastases of tumors.

Lab Personnel

Betsy Buechler, CAMB Student
Elizabeth Feeser, Post-doctoral
Michael Greenberg, Post-doctoral
Tianming Lin, Research Specialist
Serapion Pyrpassopoulos, Post-doctoral
Abbey Weith, Post-doctoral
Allison Zajac, CAMB Student

Selected Publications

H. Shuman, M.J. Greenberg, A. Zwolak, T. Lin, C.V. Sindelar, R. Dominguez, and E.M. Ostap: A Vertebrate Myosin-I Structure Reveals Unique Insights into Myosin Mechanochemical Tuning. PNAS 111(6): 2116-21, Feb 2014.

A. Zwolak, C. Yang, E.A. Feeser, E.M. Ostap, T. Svitkina, R. Dominguez: CARMIL leading edge localization depends on a non-canonical PH domain and dimerization. Nat Commun 4: 2523, 2013.

A.L. Zajac, Y.E. Goldman, E.L.F. Holzbaur, and E.M. Ostap: Local cytoskeletal and organelle interactions impact molecular motor-driven early endosomal trafficking. Current Biology 23(13): 1173-1180, 2013 Notes: Holzbaur and Ostap are co-corresponding authors.

M. Greenberg, T. Lin, Y.E. Goldman, H. Shuman, E.M. Ostap: Myosin-Ic generates power over a range of loads via a new tension sensing mechanism. PNAS 109(37): E2433-40, August 2012.

S. Pyrpassopoulos, E.A. Feeser, J.N. Mazerik, M.J. Tyska, E.M. Ostap: Membrane-Bound Myo1c Powers Asymmetric Motility of Actin Filaments. Current Biology 22(18): 1688-92, August 2012.

C. Chen, M.J. Greenberg, J.M. Laakso, E.M. Ostap, Y.E. Goldman, H. Shuman: Kinetic schemes for post-synchronized single molecule dynamics. Biophysical Journal 102(6): L23-L25, March 2012.

J. McKenna, Y.E. Goldman, E.M. Ostap: Sites of Glucose Transporter-4 Vesicle Fusion with the Plasma Membrane Correlate Spatially with Microtubules. PloS One 7(8): e43662, 2012.

J.H. Lewis, M.J. Greenberg, J.M. Laakso, H. Shuman, E.M. Ostap: Calcium regulation of myosin-I tension sensing. Biophysical Journal 102(12): 2799-807, 2012.

T. Lin, M.J. Greenberg, J.R. Moore, and E.M. Ostap: A Hearing-Loss Associated Myo1c Mutation (R156W) Decreases the Myosin Duty Ratio and Force Sensitivity. Biochemistry In Press, Feb 2011.

Pyrpassopoulos, S., Shuman, H., Ostap, E.M.: Single-molecule adhesion forces and attachment lifetimes of myosin-I phosphoinositide interactions. Biophysical Journal 99(12): 3916-22, Dec 2010.

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Last updated: 03/21/2014
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