Oxidative stress, carcinogenesis, cardiovascular disease, and neurodegenerative disease
Oxidative stress results in damage to lipids through the formation of lipid hydroperoxides. Our laboratory is involved in determining the factors that control lipid hydroperoxide-mediated damage to DNA, and proteins. We are also characterizing the lesions in these macromolecules using novel mass spectrometry methodology, determining how the lesions affect proliferation and apoptosis using model in vitro systems, and assessing how such processes can be prevented using novel pharmacological agents. We also have a program in biomarker discovery and analysis, which is focused on metabolomic and proteomic biomarkers of smoking, pancreatic cancer, breast cancer, and neurodegenerative diseases. This involves the use of quantitative stable isotope dilution liquid chromatography-mass spectrometry methodology. We recently implement the use of stable isotope dilution by essential nutrients in cell culture (SILEC) as a method for analyzing important Krebs cycle coenzyme A-thioesters. This has made it possible to examine the effects of chemicals and disease on mitochondrial dysfunction.
Darpolor MM, Basu SS, Worth A, Nelson DS, Clarke-Katzenberg RH, Glickson JD, Kaplan DE, Blair IA. : The aspartate metabolism pathway is differentiable in human hepatocellular carcinoma: transcriptomics and 13 C-isotope based metabolomics. NMR Biomed. 27(4): 381-9, 2014.
Huang M, Zhang L, Mesaros CA, Zhang S, Blaha M, Blair IA, Penning TM. : Metabolism of a Representative Oxygenated Polycyclic Aromatic Hydrocarbon (PAH) Phenanthrene-9,10-quinone in Human Hepatoma (HepG2) Cells. Chem Res Toxicol. 2014 Notes: 2014 Mar 19. [Epub ahead of print]
Tamae D, Byrns M, Marck B, Mostaghel EA, Nelson PS, Lange P, Lin D, Taplin ME, Balk S, Ellis W, True L, Vessella R, Montgomery B, Blair IA, Penning TM. : Development, validation and application of a stable isotope dilution liquid chromatography electrospray ionization/selected reaction monitoring/mass spectrometry (SID-LC/ESI/SRM/MS) method for quantification of keto-androgens in human serum. J Steroid Biochem Mol Biol. 138C: 281-289, July 2013.
Lee I, Dodia C, Chatterjee S, Zagorski J, Mesaros C, Blair IA, Feinstein SI, Jain M, Fisher AB.: A novel non-toxic inhibitor of the activation of NADPH oxidase (NOX2) reduces reactive oxygen species production in mouse lung. J Pharmacol Exp Ther. 345(2): 284-96, 2013.
Basu SS, Deutsch EC, Schmaier AA, Lynch DR, Blair IA.: Human platelets as a platform to monitor metabolic biomarkers using stable isotopes and LC-MS. Bioanalysis 5(24): 3009-21, 2013.
319. Baldwin DA, Sarnowski CP, Reddy SA, Blair IA, Clapper M, Lazarus P, Li M, Muscat JE, Penning TM, Vachani A, Whitehead AS: Development of a genotyping microarray for studying the role of gene-environment interactions in risk for lung cancer. J Biomol Tech 24(4): 198-217, 2013.
Strasser AA, Ashare RL, Kaufman M, Tang KZ, Mesaros C, Blair IA: The effect of menthol on cigarette smoking behaviors, biomarkers and subjective responses. Cancer Epidemiol Biomarkers Prev. 22(3): 382-389, 2013.
Tamae D, Byrns M, Marck B, Mostaghel EA, Nelson PS, Lange P, Lin D, Taplin ME, Balk S, Ellis W, True L, Vessella R, Montgomery B, Blair IA, Penning TM. : Development, validation and application of a stable isotope dilution liquid chromatography electrospray ionization/selected reaction monitoring/mass spectrometry (SID-LC/ESI/SRM/MS) method for quantification of keto-androgens in human serum. J Steroid Biochem Mol Biol. (138C), 281-289, 2013.
Snyder N, Khezam M, Mesaros AC, Worth A, and Blair IA. : Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography- High Resolution Mass Spectrometry (UPLC-HRMS). J Vis Exp.(75), e50433, 2013 Notes: doi: 10.3791/50433.
Huang M, Blair IA, Penning TM. : Identification of stable benzo[a]pyrene-7,8-dione-DNA adducts in human lung cells. Chem Res Toxicol 26(5): 685-692, 2013.
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Last updated: 04/09/2014
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