RESEARCH & TECHNICAL EXPERTISE
Microbiology and Immunology
•Expertise in vaccine development.
•General virology techniques including, production, purification and titration, strategy to create mutant viruses, making pseudo viruses, testing, etc.
•Experties in development of animal models for evaluation of infectious agents and vaccines. Developed humanized mouse model for HSV-2 genital infections.
•General immunological techniques for example antibody response using ELISA, neutralization assay, binding assays, rosetting assay for IgG binding, FACS analysis.
•Tissue and cell culture techniques, maintenance of cell lines, Transfection studies, Phase contrast, Immuno-fluorescent and confocal-microscopy.
Protein Chemistry/ Proteomics
•Protein purification using affinity chromatography and HPLC.
•Recombinant protein expression in E. coli, insect cells and yeast, Structure-function relationship.
•Protein electrophoresis, 2-Dimentional gel electrophoretic analysis, protein-protein interactions, protein phosphorylation, phosphoaminoacid analysis.
•Production and characterization of monoclonal and polyclonal antibodies.
•Hands on experience with GCG as well as new bioinformatics programs for computational analysis of proteins to predict liner functional motif or structure.
Molecular and Cellular Biology
•Cloning strategy, Northern and Southern blotting, Invirto transcription and translation, RNA manipulation, Protein-RNA interactions, Site directed mutagenesis, RT-PCR quantitation, Gel shift assay, RNAse protection, In situ hybridization, Micro array, Two-hybrid assay.
•Knowledge of signaling pathways induced under stress conditions and their assays.
•Hands on experience with GCG as well as new Bioinformatics program for computational analysis of DNA and RNA. I submitted three new splice variants m-RNA sequence on gene bank.
General Lab practices
•Hands on experience with medical, pharmaceutical, clinical and basic science research information retrieval from various data bases.
•Trained for handling radioactivity.
•Knowledge of GLP and good record keeping.
•Supervised technical staff and undergraduate and graduate students at University of Pennsylvania and other institutions.
•Experienced in writing animal protocols for approval from IUCAC.
Awasthi S., Mahairas G., Shaw C.E. Huang M.L., Posavad C.,Corey L., and Friedman H.M.: Evaluation of a dual modality HSV-2 vaccine approach with Ad28/35::UL19/UL47 and gD2/gC2 subunits in mice and guinea pigs. IHW 2014 39: 60, July 2014.
Friedman H.M., Belshe R.B., Awasthi S.: Better neutralization of HSV-1 than HSV-2 in subjects immunized with the GSK gD2 subunit vaccine IHW 39: 53, July 2014.
Awasthi S, Friedman HM: Status of prophylactic and therapeutic genital herpes vaccines. Current Opinion in Virology 6: 6-12, June 2014.
Awasthi S., Huang J., Shaw C.E., Friedman H.M.: Blocking Herpes Simplex Virus 2 Glycoprotein E Immune Evasion as an Approach To Enhance Efficacy of a Trivalent Subunit Antigen Vaccine for Genital Herpes
J. Virol. (2014) 88 (15): 8421-8432 88(15): 8421-8432, May 2014 Notes: Published ahead of print.
Awasthi S. Belshe R. Friedman H. F. : Better neutralization of HSV-1 than HSV-2 by antibody from subjects immunized with the GlaxoSmithKline gD2 vaccine. Journal of Infectious Disease 210(4), March 2014 Notes: In Press.
Awasthi S.,and Friedman, H.: A Paradigm Shift: Vaccine-Induced Antibodies as an Immune Correlate of Protection against Herpes Simplex Virus Type 1 Genital Herpes
Journal of Infectious Disease.
(eds.). 209: 813-815, March 2014 Notes: Epub ahead of print.
Awasthi S., Balliet J.W., Flynn J A , Lubinski J M , Shaw C E, Distefano D J, Cai M, Brown M, Smith J F, Kowalski R, Swoyer R, Galli J, Copeland V, Rios S, Davidson R C, Salnikova M, Kingsley S , Bryan J, Casimiro D R and Friedman H M. : Protection Provided by an HSV-2 Glycoprotein C and D Subunit Antigen Vaccine against Genital HSV-2 Infection in HSV-1 Seropositive Guinea Pigs. Journal of Virology 88(4): 2000-2010, February 2014.
Awasthi S., Shaw C.E., Friedman H.M.: Improving immunogenicity and efficacy of vaccines for genital herpes containing herpes simplex virus glycoprotein D. Expert Review in Vaccines 13(12), 2014 Notes: In press.
Awasthi S., Shaw C.E., Friedman H.M.: HSV-2 glycoproteins C, D and E as a trivalent subunit antigen vaccine for prevention and treatment of genital herpes. ISV 8, 2014.
Sita Awasthi: Immune Evasion by Persistent Viruses and Cancers: Blocking Evasion as a
Rational Design to Treat Viral Infections and Cancers. Journal of Antivirals and antiretrovirals 3(5), November 2013.
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Last updated: 08/19/2014
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