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Sita Awasthi, Ph.D.

Research Assistant Professor of Medicine
Department: Medicine

Contact information
522F Johnson Pavilion
3610 Hamilton walk
Philadelphia, PA 19104
Office: 215-573-8422
Fax: 215-349-5111
Lab: 215-573-8444
Education:
BS (Biology and Chemistry )
Devi Ahilya University, Indore, India , 1982.
MS (Biochemistry)
Devi Ahilya University, Indore, India , 1984.
PhD (Biochemistry)
Devi Ahilya University, Indore, India , 1989.
Post-Graduate Training
Postdoctoral fellow, Department of Pathology, University of Texas Medical Branch, Galveston, TX, 1989-1991.
Visiting fellow, NIH/NIAID Rocky Mountain Laboratories (RML), Hamilton, Montana, 1991-1993.
Research Associate, Baylor College of Medicine, Houston TX, 1993-1995.
Research Associate II, Cancer Center, Health Science Center, University of New Mexico, Albuquerque, NM, 1995-1997.
Postdoctoral fellow, Cancer Biology Department, School of Medicine, University of Pennsylvania, Philadelphia, PA, 1997-2002.
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Description of Research Expertise

Major area of my research interests are development of vaccine against genital herpes infection, development of new, and modulate existing animal models to learn and evaluate treatment and prevention options for genital herpes, and HIV co-infections, Point of Care diagnostic for HSV-2 infections for resource limited settings.

TECHNICAL EXPERTISE
Microbiology and Immunology
Expertise in vaccine development
General virology techniques including, production, purification and titration, strategy to create mutant viruses, making pseudo viruses, testing
Expertise in development of animal models for evaluation of infectious agents and vaccines-Developed humanized mouse model for HSV-2 genital infections
General immunological techniques for example antibody response using ELISA, neutralization assay, binding assays, rosetting assay for IgG binding, FACS analysis
Tissue and cell culture techniques, maintenance of cell lines, Transfection studies, Phase contrast, Immuno-fluorescent and confocal-microscopy

Protein Chemistry/ Proteomics
Protein purification using affinity chromatography and HPLC
Recombinant protein expression in E. coli, insect cells and yeast, Structure-function relationship
Protein electrophoresis, 2-Dimentional gel electrophoretic analysis, protein-protein interactions, protein phosphorylation, phosphoaminoacid analysis
Production and characterization of monoclonal and polyclonal antibodies
Hands on experience with bioinformatics programs for computational analysis of proteins to predict liner functional motif or structure Molecular and Cellular Biology
Cloning strategy, Northern and Southern blotting,Invirto transcription and translation, RNA manipulation, Protein-RNA interactions, Site directed mutagenesis, RT-PCR quantitation, Gel shift assay, RNAse protection, In situ hybridization, Micro array, Two-hybrid assay
Knowledge of signaling pathways induced under stress conditions and their assays
Hands on experience with Bioinformatics program for computational analysis of biomolecules

General Lab practices
Hands on experience with medical, pharmaceutical, clinical and basic science research information retrieval from various data bases
Trained for handling radioactivity
Knowledge of GLP and good record keeping
Experienced in writing animal protocols for approval from IUCAC

Selected Publications

Awasthi S, Hook LM, Veazey R, Gardner M, Pahar B, Friedman HM: Immunogenicity of a trivalent subunit vaccine for genital herpes in Rhesus macaques 40th International Herpes Workshop, Boise, ID 40, July 2015 Notes: Poster Presentation.

Awasthi S, Hook LM, Veazey R, Gardner M, Pahar B, Friedman HM: Immunogenicity of a trivalent vaccine for genital herpes in Rhesus macaques. Department of Medicine Research Day, Perelman School of Medicine May 2015 Notes: Poster Presentation.

Morçöl1 T, Philip R, Awashti S, Friedman HM : Novel Calcium Phosphate Nanoparticle (CaPNP) Vaccine Adjuvant and Delivery System. New Technologies, New Vaccines 2015 Meeting, Newark, DE 10, March 2015 Notes: Poster Presentation.

Awasthi S, Veazey R, Gardner M, Pahar B, Friedman HM: Evaluation of immunogenicity of a HSV-2 trivalent vaccine in rhesus macaque Penn CFAR Research Retreat, Perelman School of Medicine December 2014 Notes: Poster Presentation.

Awasthi S, Shaw CE, Friedman HM: Improving immunogenicity and efficacy of vaccines for genital herpes containing herpes simplex virus glycoprotein D. Expert Review in Vaccines 13(12): 1-14, Aug 2014.

Awasthi S, Mahairas G, Shaw CE, Huang ML, Posavad C, Corey L, Friedman HM: Evaluation of a dual modality HSV-2 vaccine approach with Ad28/35::UL19/UL47 and gD2/gC2 subunits in mice and guinea pigs. 39th International Herpesvirus Workshop 2014, Kobe, Japan 39: 60, July 2014 Notes: Poster Presentation.

Friedman HM, Belshe RB, Awasthi S: Better neutralization of HSV-1 than HSV-2 in subjects immunized with the GSK gD2 subunit vaccine 39th International Herpesvirus Workshop, Kobe, Japan 39: 53, July 2014 Notes: Poster Presentation.

Awasthi S, Friedman HM: Status of prophylactic and therapeutic genital herpes vaccines. Current Opinion in Virology 6: 6-12, June 2014.

Awasthi S, Huang J, Shaw CE, Friedman HM: Blocking herpes simplex virus 2 glycoprotein E immune evasion as an approach to enhance efficacy of a trivalent subunit antigen vaccine for genital herpes J Virol 88(15): 8421-8432, May 2014.

Awasthi S, Belshe R, Friedman HM : Better neutralization of HSV-1 than HSV-2 by antibody from subjects immunized with the GlaxoSmithKline gD2 vaccine. J Infect Dis 210(4), March 2014.

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Last updated: 05/27/2015
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