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Faculty

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Sita Awasthi, Ph.D.

Research Associate Professor of Medicine
Department: Medicine

Contact information
522F Johnson Pavilion
3610 Hamilton Walk
Philadelphia, PA 19104
Office: 215-573-8422
Fax: 215-349-5111
Lab: 215-573-8444
Education:
BS (Biology and Chemistry )
Devi Ahilya University, Indore, India , 1982.
MS (Biochemistry)
Devi Ahilya University, Indore, India , 1984.
PhD (Biochemistry)
Devi Ahilya University, Indore, India , 1989.
Post-Graduate Training
Postdoctoral fellow, Department of Pathology, University of Texas Medical Branch, Galveston, TX, 1989-1991.
Visiting fellow, NIH/NIAID Rocky Mountain Laboratories (RML), Hamilton, Montana, 1991-1993.
Research Associate, Baylor College of Medicine, Houston TX, 1993-1995.
Research Associate II, Cancer Center, Health Science Center, University of New Mexico, Albuquerque, NM, 1995-1997.
Postdoctoral fellow, Cancer Biology Department, School of Medicine, University of Pennsylvania, Philadelphia, PA, 1997-2002.
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Description of Research Expertise

Major area of my research interests are development of vaccine against genital herpes infection, development of new, and modulate existing animal models to learn and evaluate treatment and prevention options for genital herpes, and HIV co-infections, Point of Care diagnostic for HSV-2 infections for resource limited settings.

TECHNICAL EXPERTISE
Microbiology and Immunology
Expertise in vaccine development
General virology techniques including, production, purification and titration, strategy to create mutant viruses, making pseudo viruses, testing
Expertise in development of animal models for evaluation of infectious agents and vaccines-Developed humanized mouse model for HSV-2 genital infections
General immunological techniques for example antibody response using ELISA, neutralization assay, binding assays, rosetting assay for IgG binding, FACS analysis
Tissue and cell culture techniques, maintenance of cell lines, Transfection studies, Phase contrast, Immuno-fluorescent and confocal-microscopy

Protein Chemistry/ Proteomics
Protein purification using affinity chromatography and HPLC
Recombinant protein expression in E. coli, insect cells and yeast, Structure-function relationship
Protein electrophoresis, 2-Dimentional gel electrophoretic analysis, protein-protein interactions, protein phosphorylation, phosphoaminoacid analysis
Production and characterization of monoclonal and polyclonal antibodies
Hands on experience with bioinformatics programs for computational analysis of proteins to predict liner functional motif or structure Molecular and Cellular Biology
Cloning strategy, Northern and Southern blotting,Invirto transcription and translation, RNA manipulation, Protein-RNA interactions, Site directed mutagenesis, RT-PCR quantitation, Gel shift assay, RNAse protection, In situ hybridization, Micro array, Two-hybrid assay
Knowledge of signaling pathways induced under stress conditions and their assays
Hands on experience with Bioinformatics program for computational analysis of biomolecules

General Lab practices
Hands on experience with medical, pharmaceutical, clinical and basic science research information retrieval from various data bases
Trained for handling radioactivity
Knowledge of GLP and good record keeping
Experienced in writing animal protocols for approval from IUCAC

Selected Publications

Shih-Chuan Liaoa, Jing Penga, Michael G. Mauka, Sita Awasthi, Jinzhao Songa, Harvey Friedman, Haim Bau, Changchun Liua: Smart Cup: Minimally-Instrumented, Smartphone-Based Point-of-Care Molecular Diagnostics Sensors and Actuators B: Chemical 2015 Notes: Submitted.

Awasthi S., Friedman H M.: Molecular association of herpes simplex virus type 1 glycoprotein E with membrane protein Us9 PLOS One 2015 Notes: Submitted the revised version.

Awasthi S, Mahairas GG, Shaw CE, Huang ML, Koelle DM, Posavad C, Corey L, and Friedman HM. : A dual modality HSV-2 vaccine for preventing genital herpes using glycoproteins C and D subunit antigens to induce potent antibody responses and adenovirus vectors containing capsid and tegument proteins as T cell immunogens. J Virol 2015 Notes: In Press.

Awasthi S, Huang J, Shaw CE, Friedman HM: Blocking herpes simplex virus 2 glycoprotein E immune evasion as an approach to enhance efficacy of a trivalent subunit antigen vaccine for genital herpes J Virol 88(15): 8421-8432, May 2014.

Awasthi S, Belshe R, Friedman HM : Better neutralization of HSV-1 than HSV-2 by antibody from subjects immunized with the GlaxoSmithKline gD2 vaccine. J Infect Dis 210(4): 571-575, March 2014.

Awasthi S, Balliet JW, Flynn JA, Lubinski JM, Shaw CE, Distefano DJ, Cai M, Brown M, Smith JF, Kowalski R, Swoyer R, Galli J, Copeland V, Rios S, Davidson RC, Salnikova M, Kingsley S, Bryan J, Casimiro DR, Friedman HM : Protection Provided by an HSV-2 Glycoprotein C and D Subunit Antigen Vaccine against Genital HSV-2 Infection in HSV-1 Seropositive Guinea Pigs. J Virol 88(4): 2000-2010, Feb 2014.

Kamoun M, Filali M, Murray MV, Awasthi S, Wadzinski BE : Protein phosphatase 2A family members (PP2A and PP6) associate with U1 snRNP and the spliceosome during pre-mRNA splicing. Biochem Biophys Res Commun. (eds.). 440 (2): 306-311, Oct 2013.

Shedlock DJ, Talbott KT, Wu SJ, Wilson CM, Muthumani K, Boyer JD, Sardesai NY, Awasthi S, Weiner DB: Vaccination with synthetic constructs expressing cytomegalovirus immunogens is highly T cell immunogenic in mice. Human Vaccines & Immunotherapeutics 8(11): 1-14, Nov 2012.

Awasthi S, Zumbrun EE, Si H, Wang F, Shaw CE, Cai M, Lubinski JM, Barrett SM, Balliet JW, Flynn JA, Casimiro DR, Bryan JT, Friedman HM: Live attenuated herpes simplex virus 2 glycoprotein E deletion mutant as a vaccine candidate defective in neuronal spread J Virol 86(8): 4586-4598, Apr 2012.

Awasthi S, Lubinski JM, Shaw CE, Barrett SM, Cai M, Wang F, Betts M, Kingsley S, DiStefano DJ, Balliet JW, Flynn JA, Casimiro DR, Bryan JT, Friedman, HM: Immunization with a Vaccine Combining Herpes Simplex Virus 2 (HSV-2) Glycoprotein C (gC) and gD Subunits Improves the Protection of Dorsal Root Ganglia in Mice and Reduces the Frequency of Recurrent Vaginal Shedding of HSV-2 DNA in Guinea Pigs Compared to Immunization with gD Alone. J Virol 85(20): 10472-10486, Oct 2011.

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Last updated: 02/04/2016
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