Major focus of my research are
1. Development of genital herpes vaccine
2. Development of new animal models, or modulation of existing animal models to learn and evaluate treatment and prevention options for genital herpes, and HIV co-infections.
3. Point of Care diagnostic for HSV-2 infections for resource limited settings.
Expertise in vaccine development.
General virology techniques including, production, purification and titration of virus.
Strategy to create mutant viruses and in-vivo evaluation.
Expertise in development of animal models for evaluation of infectious agents and vaccines.
Developed humanized mouse model for HSV-2 genital infections.
Evaluation of immune responses.
Evaluation and blocking of evasion of host immune response.
Awasthi S, Friedman HM: Molecular association of herpes simplex virus type 1 glycoprotein E with membrane protein Us9. Archives of Virology 161(11): 3203-13, Nov 2016.
Shih-Chuan Liaoa, Jing Penga, Michael G. Mauka, Sita Awasthi, Jinzhao Songa, Harvey Friedman, Haim Bau, Changchun Liua: Smart Cup: Minimally-Instrumented, Smartphone-Based Point-of-Care Molecular Diagnostics Sensors and Actuators B: Chemical 2015 Notes: Submitted.
Awasthi S, Mahairas GG, Shaw CE, Huang ML, Koelle DM, Posavad C, Corey L, and Friedman HM. : A dual modality HSV-2 vaccine for preventing genital herpes using glycoproteins C and D subunit antigens to induce potent antibody responses and adenovirus vectors containing capsid and tegument proteins as T cell immunogens. J Virol 2015 Notes: In Press.
Awasthi S, Huang J, Shaw CE, Friedman HM: Blocking herpes simplex virus 2 glycoprotein E immune evasion as an approach to enhance efficacy of a trivalent subunit antigen vaccine for genital herpes
J Virol 88(15): 8421-8432, May 2014.
Awasthi S, Belshe R, Friedman HM : Better neutralization of HSV-1 than HSV-2 by antibody from subjects immunized with the GlaxoSmithKline gD2 vaccine. J Infect Dis 210(4): 571-575, March 2014.
Awasthi S, Balliet JW, Flynn JA, Lubinski JM, Shaw CE, Distefano DJ, Cai M, Brown M, Smith JF, Kowalski R, Swoyer R, Galli J, Copeland V, Rios S, Davidson RC, Salnikova M, Kingsley S, Bryan J, Casimiro DR, Friedman HM : Protection Provided by an HSV-2 Glycoprotein C and D Subunit Antigen Vaccine against Genital HSV-2 Infection in HSV-1 Seropositive Guinea Pigs. J Virol 88(4): 2000-2010, Feb 2014.
Kamoun M, Filali M, Murray MV, Awasthi S, Wadzinski BE : Protein phosphatase 2A family members (PP2A and PP6) associate with U1 snRNP and the spliceosome during pre-mRNA splicing. Biochem Biophys Res Commun.
(eds.). 440 (2): 306-311, Oct 2013.
Shedlock DJ, Talbott KT, Wu SJ, Wilson CM, Muthumani K, Boyer JD, Sardesai NY, Awasthi S, Weiner DB: Vaccination with synthetic constructs expressing cytomegalovirus immunogens is highly T cell immunogenic in mice. Human Vaccines & Immunotherapeutics 8(11): 1-14, Nov 2012.
Awasthi S, Zumbrun EE, Si H, Wang F, Shaw CE, Cai M, Lubinski JM, Barrett SM, Balliet JW, Flynn JA, Casimiro DR, Bryan JT, Friedman HM: Live attenuated herpes simplex virus 2 glycoprotein E deletion mutant as a vaccine candidate defective in neuronal spread J Virol 86(8): 4586-4598, Apr 2012.
Awasthi S, Lubinski JM, Shaw CE, Barrett SM, Cai M, Wang F, Betts M, Kingsley S, DiStefano DJ, Balliet JW, Flynn JA, Casimiro DR, Bryan JT, Friedman, HM: Immunization with a Vaccine Combining Herpes Simplex Virus 2 (HSV-2) Glycoprotein C (gC) and gD Subunits Improves the Protection of Dorsal Root Ganglia in Mice and Reduces the Frequency of Recurrent Vaginal Shedding of HSV-2 DNA in Guinea Pigs Compared to Immunization with gD Alone. J Virol 85(20): 10472-10486, Oct 2011.
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Last updated: 10/19/2016
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