Bayard T. Storey
421 Curie Blvd.
Philadelphia, PA 19104
Harvard University, 1952.
Massachusetts Institute of Technology, 1955.
Harvard University, 1958.
Description of Research ExpertiseOne area of research is the sequence and mechanisms of the reactions leading to fertilization of mouse eggs by mouse sperm, the initial one of which is the sperm acrosome reaction induced by the egg's zona pellucida in capacitated sperm. The time course, nature, and sequence of the different reactions, which define one or more intracellular signal transduction pathways, have been elucidated by in situ dissection with fluorescent probes and specific inhibitors/activators. More recently, collaboration with Dr George Gerton's group has utilised the transgenic mouse line that has acrosomal Green Fluorescent Protein.
The study of lipid peroxidation in mammalian sperm, more recently in human sperm, has spanned two decades and is now effectively complete.
A third study, now complete, has dealt with cryopreservation of mouse and human sperm.
Selected PublicationsCalamera, J.C., Buffone, M.G., Doncel, G.F., Brugo-Olmedo, S., de Vincentis, S., Calamera, M.M., Storey, B.T., Alvarez, J.G.: Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa. Fertility and Sterility 93: 789-794, February 2010.
Buffone, M.G., Rodriguez-Miranda, E., Storey, B.T., Gerton, G.L. : Acrosomal Exocytosis of mouse sperm progresses in a consistent direction in response to zona Pellucida. Journal of Cellular Physiology 220: 611-620, April 2009.
Storey, B.T.: Mammalian sperm metabolism: oxygen and sugar, friend and foe. International Journal of Developmental Biology 52(5/6): 427-437, 2008.
Galantino-Homer, H.L., Florman, H.M., Storey, B.T., Dobrinski, I., Kopf, G.S.: Bovine sperm capacitation: assessment of phosphodiesterase activity and intracellular alkalinization-associated protein tyrosine phosphorylation. Molecular Reproduction & Development 67: 487-500, 2004.
Selak MA, Storey BT, Peterside I, Simmons RA.: Impaired oxidative phosphorylation in skeletal muscle of intrauterine growth-retarded rats. Am J Physiol Endocrinol Metab. 285(1): E130-7, July 2003.
Thompson KA, Richa J, Liebhaber SA, Storey BT.: Dialysis addition of trehalose/glycerol cryoprotectant allows recovery of cryopreserved mouse spermatozoa with satisfactory fertilizing ability as assessed by yield of live young. J Androl. 22(2): 339-44, March-April 2001.
Travis AJ, Jorgez CJ, Merdiushev T, Jones BH, Dess DM, Diaz-Cueto L, Storey BT, Kopf GS, Moss SB: Functional relationships between capacitation-dependent cell signaling and compartmentalized metabolic pathways in murine spermatozoa. J Biol Chem. 276(10): 7630-6, March 9 2001.
Rockwell PL, Storey BT: Kinetics of onset of mouse sperm acrosome reaction induced by solubilized zona pellucida: fluorimetric determination of loss of pH gradient between acrosomal lumen and medium monitored by dapoxyl (2-aminoethyl) sulfonamide and of intracellular Ca(2+) changes monitored by fluo-3. Mol Reprod Dev. 55(3): 335-49, March 2000.
Rockwell PL, Storey BT: Determination of the intracellular dissociation constant, K(D), of the fluo-3. Ca(2+) complex in mouse sperm for use in estimating intracellular Ca(2+)concentrations. Mol Reprod Dev. 54(4): 418-28, December 1999.
Storey BT, Alvarez JG, Thompson KA: Human sperm glutathione reductase activity in situ reveals limitation in the glutathione antioxidant defense system due to supply of NADPH. Mol Reprod Dev. 49(4): 400-407. April 1998.