3400 Civic Center Blvd, Bldg 421
Office: TRC 8-101; Lab: TCR 8-194~196
Philadelphia, PA 19104
Description of Research ExpertiseDescription of Research
The human genome contains ~25,000 protein-coding genes representing less than 2% of the total genome, whereas up to 70% of the human genome is transcribed to RNA, yielding many thousands of non-coding RNAs. The recent discovery of non-coding RNA genes has dramatically altered our understanding on cancer. Long non-coding RNAs (lncRNAs) are operationally defined as RNA genes larger than 200 nucleotide that do not appear to have protein coding potential. micoRNAs (miRNAs) are endogenous ~18-25 nucleotide non-coding small RNAs which regulate gene expression in a sequence-specific manner via the degradation of target mRNAs or the inhibition of protein translation. The Zhang laboratory is interested in molecular mechanisms of non-coding RNAs (microRNA and lncRNA) in tumorigenesis, and the potential application of non-coding RNA in treatment for breast and ovarian cancer. High-throughput profiling technologies (microarray and deep-sequencing) and whole-genome wide miRNA/siRNA screening are used in the laboratory.
OncoGenome and EpiGenome in ovarian and breast cancers
microRNAs/long non-coding RNAs in cancer
microRNA-based therapeutic and diagnostic strategies in cancer
microRNAs in cancer stem cells and embryonic stem cells
Zhang Lab Members
One technician (Lab manager)
Four postdoc researchers
Selected PublicationsLi N, Zhong X, Lin X, Guo J, Zou L, Tanyi JL, Shao Z, Liang S, Wang LP, Hwang WT, Katsaros D, Montone K, Zhao X, Zhang L.: lin-28 homologue A (LIN28A) promotes cell cycle progression via the regulation of cyclin-dependent kinase 2 (CDK2),cyclin D1 (CCND1), and cell division cycle 25 homolog A (CDC25A) expression in cancer. J Biol Chem. 2012.
Li N, Kaur S, Greshock J, Lassus H, Zhong X, Wang Y, Leminen A, Shao Z, Hu X, Liang S, Katsaros D, Huang Q, Bützow R, Weber BL, Coukos G, Zhang L. : A combined array-based comparative genomic hybridization (aCGH) and functional library screening approach identifies mir-30d as an oncomir in cancer. Cancer Res. Nov 4. [Epub ahead of print], 2011.
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G.: Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature 13;475(7355):226-30, 2011.
Yang X, Lin X, Zhong X, Kaur S, Li N, Liang S, Lassus H, Wang L, Katsaros D, Montone K, Zhao X, Zhang Y, Bützow R, Coukos G, Zhang L: Double negative feedback loop between reprogramming factor LIN28 and microRNA let-7 regulates aldehyde dehydrogenase 1-positive cancer stem cells. Cancer Res 15:70(22):9463-72, 2010.
Zhong X, Li N, Liang S, Huang Q, Coukos G, Zhang L: Identification of microRNAs regulating reprogramming factor LIN28 in embryonic stem cells and cancer cells. J Biol Chem 31:285(53):41961-71, 2010.
Gumireddy K, Li A, Gimotty PA, Klein-Szanto AJ, Showe LC, Katsaros D, Coukos G, Zhang L, Huang Q: KLF17 is a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Nat Cell Biol 11(11):1297-304, Nov 2009.
Yang N, Kaur S, Volinia S, Greshock J, Lassus H, Hasegawa K, Liang S, Leminen A, Deng S, Smith L, Johnstone CN, Chen XM, Liu CG, Huang Q, Katsaros D, Calin GA, Weber BL, Bützow R, Croce CM, Coukos G, Zhang L: MicroRNA microarray identifies Let-7i as a novel biomarker and therapeutic target in human epithelial ovarian cancer. Cancer Research 68(24):10307-14, Dec 2008.
Zhang L, Volinia S, Bonome T, Calin GA, Greshock J, Yang N, Liu CG, Giannakakis A, Alexiou P, Hasegawa K, Johnstone CN, Megraw MS, Adams S, Lassus H, Huang J, Kaur S, Liang S, Sethupathy P, Leminen A, Simossis VA, Sandaltzopoulos R, Naomoto Y, Katsaros D, Gimotty PA, DeMichele A, Huang Q, Bützow R, Rustgi AK, Weber BL, Birrer MJ, Hatzigeorgiou AG, Croce CM, Coukos G: Genomic and epigenetic alterations deregulate microRNA expression in human epithelial ovarian cancer. Proceed Nat Acad Sciences of USA 105(19):7004-09, May 2008.
Zhang L, Huang J, Yang N, Greshock J, Megraw MS, Giannakakis A, Liang S, Naylor TL, Barchetti A, Ward MR, Yao G, Medina A, O'Brien-Jenkins A, Katsaros D, Hatzigeorgiou A, Gimotty PA, Weber BL, Coukos G: microRNAs exhibit high frequency genomic alterations in human cancer. Proceed Natl Acad Sciences of USA 103(24):9136-41, Jun 2006.
Zhang L, Conejo-Garcia JR, Katsaros D, Gimotty PA, Massobrio M, Regnani G, Makrigiannakis A, Gray H, Schlienger K, Liebman MN, Rubin SC, Coukos G: Intratumoral T cells, recurrence, and survival in epithelial ovarian cancer. New Engl J Med 348(3):203-13, Jan 2003.