David J.E. Kast, Ph.D.

PostDoctoral Fellow
Ph.D. 2010 - University of Minnesota, Biophysics

E-mail: kast at mail . med . upenn . edu
Phone: (215) 573-0983

Selected Publications

Kast DJ & Dominguez R (2015) WHAMM links actin assembly via the Arp2/3 complex to autophagy. Autophagy 11:1702-1704

Kast DJ, Zajac AL, Holzbaur EL, Ostap EM, Dominguez R (2015) WHAMM directs the Arp2/3 complex to the ER for autophagosome biogenesis through and actin comet tail mechanism. Curr Biol 25:1791-1797

Kast DJ, Yang C, Disanza A, Boczkowska M, Madasu Y, Scita G, Svitkina T and Dominguez R (2014) Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors. Nature Struct Mol Biol 21:413-422.

Boczkowska M, Rebowski G, Kast DJ, Dominguez R (2014) Structural analysis of the transitional state of Arp2/3 complex activation by two actin-bound WCAs. Nature Commun 5:3308. DOI:10.1038/ncomms4308

Turegun B, Kast DJ, Dominguez R (2013) Subunit Rtt102 controls the conformation of the Arp7/9 heterodimer and its interactions with nucleotide and the catalytic subunit of SWI/SNF remodelers. J Biol Chem 288:35758-35768

Disanza A, Bisi S, Winterhoff M, Milanesi F, Ushakov DS, Kast DJ, Marighetti P, Romet-Lemonne G, Müller HM, Nickel W, Linkner J, Waterschoot D, Ampè C, Cortellino S, Palamidessi A, Dominguez R, Carlier MF, Faix J, Scita G (2013) Cdc42 switches IRSp53 from inhibition of actin growth to elongation by clustering of VASP. EMBO J 32:2735-2750

Madasu Y, Suarez C, Kast DJ, Kovar DR and Dominguez R (2013) Rickettsia Sca2 has evolved formin-like activity through a different molecular mechanism. PNAS 110:E2677-E2686

Thomas DD, Muretta JM, Colson BA, Mello R, Kast DJ (2011) Spectroscopic probes of muscle proteins. Comprehensive Biophysics, chapter 4.15. Elsevier

Kast DJ & Dominguez R (2011) Arp you ready for actin in the nucleus? EMBO J 30:2097-2098

Muretta JM, Kyrychenko A, Ladokhin AS, Kast DJ, Gillispie GD, Thomas DD (2010) High-performance time-resolved fluorescence by direct waveform recording. Rev Sci Instrum 81:103101

Kast D, Espinoza-Fonseca LM, Yi C, Thomas DD (2010) Phosphorylation-induced structural changes in smooth muscle myosin regulatory light chain. PNAS 107:8207-8212

Thomas DD, Kast D, Korman VL (2009) Site-directed spectroscopic probes of actomyosin structural dynamics. Annu Rev Biophys 38:347-369

Espinoza-Fonseca LM, Kast D, Thomas DD (2008) Thermodynamic and structural basis of phosphorylation-induced disorder-to-order transition in the regulatory light chain of smooth muscle myosin. J Am Chem Soc 130:12208-12209

Espinoza-Fonseca LM, Kast D, Thomas DD (2007) Molecular dynamics simulations reveal a disorder-to-order transition on phosphorylation of smooth muscle myosin. Biophys J 93:2083-2090