Penn Program in Epigenetics
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Research

Epigenetics

Protocols

We are soliciting protocols, from anyone in the epigenetics community, that may be useful to the community at large.  They will be posted here to be seen by a large audience and to facilitate both research and collaboration.  Please contact epigen@mail.med.upenn.edu if you would like to share!

From Shelley Berger's lab:
Isolating Old Yeast by Surface Labeling - Quadruple Sorting
Creating a ChIP Seq Library
ChIP Protocol for IMR 90 cells
qPCR for human cell RNA

Antibody website and information

This website summarizes the results of all examined antibodies and one that allows users to post their own experiences.  This is really useful to everyone in the larger community:

http://compbio.med.harvard.edu/antibodies/

This is a result of an interesting article from Jason Lieb et al. :

An assessment of histone-modification antibody quality
Egelhofer TA, Minoda A, Klugman S, Lee K, Kolasinska-Zwierz P, Alekseyenko AA, Cheung MS, Day DS, Gadel S, Gorchakov AA, Gu T, Kharchenko PV, Kuan S, Latorre I, Linder-Basso D, Luu Y, Ngo Q, Perry M, Rechtsteiner A, Richie NC, Schwartz YB, Shanower GA, Vielle A, Ahringer J, Elgin SC, Kuroda MI, Pirrotta V, Ren B, Strome S, Park PJ, Karpen GH, Hawkins RD, Lieb JD.  Nat Struct Mol Biol. 2011 Jan;18(1):91-3. Epub 2010 Dec. 5.

Roadmap Epigenomics Project

The Roadmap Epigenomics Project consists of multiple initiatives intended to further the discovery, access, and coordination of data generated by the many branches of epigenomics, as well as the application of this information toward health and disease research.  More information can be found at this link:

http://www.roadmapepigenomics.org/