Kushol Gupta, Ph.D.

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Research Assistant Professor of Biochemistry and Biophysics
Department: Biochemistry and Biophysics
Graduate Group Affiliations

Contact information
810 Stellar-Chance Building
422 Curie Blvd.
Department of Biochemistry and Biophysics
Philadelphia, PA 19104
Office: 267-259-0082
Lab: 215-573-7260
B.A. (Biochemistry and Music)
University of Pennsylvania, 1997.
Ph.D. (Pharmacology)
Perelman School of Medicine, University of Pennsylvania, 2003.
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Description of Research Expertise

The central theme of my current research is the study of large macromolecular assemblies that regulate genetic information. My investigations have included:

1. Viral proteins that mediate lateral DNA transfer, including retroviral integrases and phage-encoded site-specific recombinases.
2. Large macromolecular assemblies that regulate RNA, including the Survival of Motor Neuron (SMN) complex, bacterial group II introns, and ribosomes.
3. The nucleosome, which is the basic repeating unit of chromatin.
4. Intrinsically disordered proteins, including LEDGF/p75, SMN, and PGC1.

I routinely employ a gamut of biochemical and biophysical techniques to approach specific problems in these systems related to structure and function, with a particular expertise in and emphasis on biophysical approaches. This includes small-angle scattering, size-exclusion chromatography in-line with multi-angle light scattering (SEC-MALS), analytical ultracentrifugation and atomistic modeling methods.

Selected Publications

Gupta Kushol, Sharp Robert, Yuan Jimmy B, Li Huiguang, Van Duyne Gregory D: Coiled-coil interactions mediate serine integrase directionality. Nucleic acids research 45(12): 7339-7353, Jul 2017.

Mandali Sridhar, Gupta Kushol, Dawson Anthony R, Van Duyne Gregory D, Johnson Reid C: Control of Recombination Directionality by the Listeria Phage A118 Protein Gp44 and the Coiled-Coil Motif of Its Serine Integrase. Journal of bacteriology 199(11), Jun 2017.

Gupta K: Genetics, Epigenetics and the Molecular Marks of Stress: A Primer Child abuse: Children with Disabilities. Palusci VJ, Nazer D, Greydanus DE, and Merrick J. (eds.). Nova Science 2017.

Gupta Kushol, Turkki Vesa, Sherrill-Mix Scott, Hwang Young, Eilers Grant, Taylor Louis, McDanal Charlene, Wang Ping, Temelkoff David, Nolte Robert T, Velthuisen Emile, Jeffrey Jerry, Van Duyne Gregory D, Bushman Frederic D: Structural Basis for Inhibitor-Induced Aggregation of HIV Integrase. PLoS biology 14(12): e1002584, Dec 2016.

Eisemann Travis, McCauley Michael, Langelier Marie-France, Gupta Kushol, Roy Swati, Van Duyne Gregory D, Pascal John M: Tankyrase-1 Ankyrin Repeats Form an Adaptable Binding Platform for Targets of ADP-Ribose Modification. Structure (London, England : 1993) 24(10): 1679-1692, Oct 2016.

Arturo Emilia C, Gupta Kushol, Héroux Annie, Stith Linda, Cross Penelope J, Parker Emily J, Loll Patrick J, Jaffe Eileen K: First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer. Proceedings of the National Academy of Sciences of the United States of America 113(9): 2394-9, Mar 2016.

Fuglestad Brian, Gupta Kushol, Wand A Joshua, Sharp Kim A: Characterization of Cetyltrimethylammonium Bromide/Hexanol Reverse Micelles by Experimentally Benchmarked Molecular Dynamics Simulations. Langmuir : the ACS journal of surfaces and colloids 32(7): 1674-84, Feb 2016.

Gupta Kushol, Martin Renee, Sharp Robert, Sarachan Kathryn L, Ninan Nisha S, Van Duyne Gregory D: Oligomeric Properties of Survival Motor Neuron·Gemin2 Complexes. The Journal of biological chemistry 290(33): 20185-99, Jun 2015.

Falk Samantha J, Guo Lucie Y, Sekulic Nikolina, Smoak Evan M, Mani Tomoyasu, Logsdon Glennis A, Gupta Kushol, Jansen Lars E T, Van Duyne Gregory D, Vinogradov Sergei A, Lampson Michael A, Black Ben E: Chromosomes. CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere. Science (New York, N.Y.) 348(6235): 699-703, May 2015.

Sweeny Elizabeth A, Jackrel Meredith E, Go Michelle S, Sochor Matthew A, Razzo Beatrice M, DeSantis Morgan E, Gupta Kushol, Shorter James: The Hsp104 N-terminal domain enables disaggregase plasticity and potentiation. Molecular cell 57(5): 836-49, Mar 2015.

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Last updated: 10/26/2017
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