Kushol Gupta, Ph.D.

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Research Assistant Professor of Biochemistry and Biophysics
Department: Biochemistry and Biophysics
Graduate Group Affiliations

Contact information
810 Stellar-Chance Building
422 Curie Blvd.
Department of Biochemistry and Biophysics
Philadelphia, PA 19104
Office: 267-259-0082
Lab: 215-573-7260
Education:
B.A. (Biochemistry and Music)
University of Pennsylvania, 1997.
Ph.D. (Pharmacology)
Perelman School of Medicine, University of Pennsylvania, 2003.
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Description of Research Expertise

The central theme of my current research is the study of large macromolecular assemblies that regulate genetic information. My investigations have included:

1. Viral proteins that mediate lateral DNA transfer, including retroviral integrases and phage-encoded site-specific recombinases.
2. Large macromolecular assemblies that regulate RNA, including the Survival of Motor Neuron (SMN) complex, bacterial group II introns, and ribosomes.
3. The nucleosome, which is the basic repeating unit of chromatin.
4. Intrinsically disordered proteins, including LEDGF/p75, SMN, and PGC1.

I routinely employ a gamut of biochemical and biophysical techniques to approach specific problems in these systems related to structure and function, with a particular expertise in and emphasis on biophysical approaches. This includes small-angle scattering, size-exclusion chromatography in-line with multi-angle light scattering (SEC-MALS), analytical ultracentrifugation and atomistic modeling methods.

Selected Publications

Gupta Kushol, Martin Renee, Sharp Robert, Sarachan Kathryn L, Ninan Nisha S, Van Duyne Gregory D: Oligomeric Properties of Survival Motor Neuron·Gemin2 Complexes. The Journal of biological chemistry Jun 2015.

Falk Samantha J, Guo Lucie Y, Sekulic Nikolina, Smoak Evan M, Mani Tomoyasu, Logsdon Glennis A, Gupta Kushol, Jansen Lars E T, Van Duyne Gregory D, Vinogradov Sergei A, Lampson Michael A, Black Ben E: Chromosomes. CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere. Science (New York, N.Y.) 348(6235): 699-703, May 2015.

Sweeny Elizabeth A, Jackrel Meredith E, Go Michelle S, Sochor Matthew A, Razzo Beatrice M, DeSantis Morgan E, Gupta Kushol, Shorter James: The Hsp104 N-terminal domain enables disaggregase plasticity and potentiation. Molecular cell 57(5): 836-49, Mar 2015.

Gupta Kushol, Selinsky Barry S: Bacterial and algal orthologs of prostaglandin H₂synthase: novel insights into the evolution of an integral membrane protein. Biochimica et biophysica acta 1848(1 Pt A): 83-94, Jan 2015.

Tao Zhihua, Fusco Amanda, Huang De-Bin, Gupta Kushol, Young Kim Daniel, Ware Carl F, Van Duyne Gregory D, Ghosh Gourisankar: p100/IκBδ sequesters and inhibits NF-κB through kappaBsome formation. Proceedings of the National Academy of Sciences of the United States of America 111(45): 15946-51, Nov 2014.

Gupta Kushol, Brady Troy, Dyer Benjamin M, Malani Nirav, Hwang Young, Male Frances, Nolte Robert T, Wang Liping, Velthuisen Emile, Jeffrey Jerry, Van Duyne Gregory D, Bushman Frederic D: Allosteric inhibition of human immunodeficiency virus integrase: late block during viral replication and abnormal multimerization involving specific protein domains. The Journal of biological chemistry 289(30): 20477-88, Jul 2014.

Gupta Kushol, Contreras Lydia M, Smith Dorie, Qu Guosheng, Huang Tao, Spruce Lynn A, Seeholzer Steven H, Belfort Marlene, Van Duyne Gregory D: Quaternary arrangement of an active, native group II intron ribonucleoprotein complex revealed by small-angle X-ray scattering. Nucleic acids research 42(8): 5347-60, Apr 2014.

Desantis Morgan E, Sweeny Elizabeth A, Snead David, Leung Eunice H, Go Michelle S, Gupta Kushol, Wendler Petra, Shorter James: Conserved distal loop residues in the Hsp104 and ClpB middle domain contact nucleotide-binding domain 2 and enable Hsp70-dependent protein disaggregation. The Journal of biological chemistry 289(2): 848-67, Jan 2014.

Martin Renee, Gupta Kushol, Ninan Nisha S, Perry Kay, Van Duyne Gregory D: The survival motor neuron protein forms soluble glycine zipper oligomers. Structure (London, England : 1993) 20(11): 1929-39, Nov 2012.

Gupta Kushol, Curtis Joseph E, Krueger Susan, Hwang Young, Cherepanov Peter, Bushman Frederic D, Van Duyne Gregory D: Solution conformations of prototype foamy virus integrase and its stable synaptic complex with U5 viral DNA. Structure (London, England : 1993) 20(11): 1918-28, Nov 2012.

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Last updated: 10/29/2015
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